Transcript: Human XM_011511351.2

PREDICTED: Homo sapiens phospholipase C like 1 (inactive) (PLCL1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
PLCL1 (5334)
Length:
6654
CDS:
693..3743

Additional Resources:

NCBI RefSeq record:
XM_011511351.2
NBCI Gene record:
PLCL1 (5334)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011511351.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001503 CGACACGTTCACCCATCTTAT pLKO.1 3757 3UTR 100% 13.200 18.480 N PLCL1 n/a
2 TRCN0000235463 TCGAAGGATGTCGGTAGATTA pLKO_005 2150 CDS 100% 13.200 18.480 N PLCL1 n/a
3 TRCN0000235465 GAAGTGTCATAGAGGTAATAA pLKO_005 1861 CDS 100% 15.000 12.000 N PLCL1 n/a
4 TRCN0000001506 CGAAGTGTCATAGAGGTAATA pLKO.1 1860 CDS 100% 13.200 10.560 N PLCL1 n/a
5 TRCN0000001504 CCAGCCATTATCTCACTACTA pLKO.1 1658 CDS 100% 4.950 3.960 N PLCL1 n/a
6 TRCN0000235461 GATCTTAGCATAGCTTATTAT pLKO_005 4205 3UTR 100% 15.000 10.500 N PLCL1 n/a
7 TRCN0000235462 ATTACGAGAAGCCATAGATAT pLKO_005 3134 CDS 100% 13.200 9.240 N PLCL1 n/a
8 TRCN0000235464 CCGGCCAAATTCTCGCATTTA pLKO_005 827 CDS 100% 13.200 9.240 N PLCL1 n/a
9 TRCN0000001505 GCTCAGGAATATCGGTCTTAA pLKO.1 3077 CDS 100% 13.200 9.240 N PLCL1 n/a
10 TRCN0000010627 GCTGGCTGTGAGTTGAAGAAA pLKO.1 804 CDS 100% 5.625 3.938 N PLCL1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011511351.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13920 pDONR223 100% 98% .7% None (many diffs) n/a
2 ccsbBroad304_13920 pLX_304 0% 98% .7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000477515 CGGGTATATCCCGATTCTAAAAAC pLX_317 14.2% 98% .7% V5 (not translated due to prior stop codon) (many diffs) n/a
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