Construct: ORF ccsbBroad304_13961
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013830.1_s304c1, BRDN0000410208
- Derived from:
- ccsbBroadEn_13961
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STXBP2 (6813)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13961
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6813 | STXBP2 | syntaxin binding protein 2 | NM_001127396.3 | 99.9% | 99.8% | 1766delT |
| 2 | human | 6813 | STXBP2 | syntaxin binding protein 2 | NM_006949.4 | 99.4% | 99.3% | 245_253delAGTCGGTTC;1775delT |
| 3 | human | 6813 | STXBP2 | syntaxin binding protein 2 | NM_001272034.2 | 97.6% | 97.5% | 245_286del;1808delT |
| 4 | human | 6813 | STXBP2 | syntaxin binding protein 2 | NR_073560.2 | 86.9% | (many diffs) | |
| 5 | human | 6813 | STXBP2 | syntaxin binding protein 2 | XR_001753741.2 | 82.7% | (many diffs) | |
| 6 | human | 6813 | STXBP2 | syntaxin binding protein 2 | XM_011528210.2 | 77.4% | 75.8% | (many diffs) |
| 7 | human | 6813 | STXBP2 | syntaxin binding protein 2 | XM_011528212.3 | 72.4% | 69.4% | (many diffs) |
| 8 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | NM_011503.4 | 87.2% | 94% | (many diffs) |
| 9 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XM_006508742.1 | 83.9% | 90.5% | (many diffs) |
| 10 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XM_017312632.1 | 82.3% | 88.7% | (many diffs) |
| 11 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XM_006508743.3 | 80.5% | 86.5% | (many diffs) |
| 12 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XR_001778417.1 | 59.3% | (many diffs) | |
| 13 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XR_001778418.1 | 59.2% | (many diffs) | |
| 14 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XR_001778419.1 | 58.2% | (many diffs) | |
| 15 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XM_006508746.1 | 53.4% | 57% | (many diffs) |
| 16 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XM_011242046.1 | 53.4% | 57% | (many diffs) |
| 17 | mouse | 20911 | Stxbp2 | syntaxin binding protein 2 | XM_017312633.1 | 44.6% | 45.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1836
- ORF length:
- 1770
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tgggcatggc gccctcgggg ctgaaggcgg tggtggggga aaaaattctg agcggagtta 121 ttcggagtgt caagaaggat ggggagtgga aggtgcttat catggatcac ccaagcatgc 181 gcatcttgtc ttcctgctgc aaaatgtcag atatcctggc tgagggcatc accattgttg 241 aagacatcaa caaacggcgg gaacccattc ccagtctgga ggccatttat ttgctgagcc 301 ccacggagaa ggccctgatc aaagacttcc aggggacccc gactttcacc tacaaagcgg 361 cccatatctt cttcaccgac acctgccccg agcccctgtt cagtgagcta ggccgctctc 421 gtctggcaaa ggtggtgaag acgttgaagg agattcacct tgccttcctc ccctacgagg 481 cccaggtgtt ctccctcgat gctccccaca gcacctacaa cctctactgc cccttccggg 541 cagaggagcg cacgcggcag ctcgaggtgc tggcccagca gattgccacg ctgtgcgcca 601 ccctgcagga gtacccggcc atccgctacc gcaagggccc agaggacaca gcccagttgg 661 cccacgccgt cctggccaag ctgaacgcct tcaaggcaga cactcccagt ctgggcgagg 721 gcccagagaa aacccgctcc cagctgctga taatggaccg ggcagctgac cccgtgtccc 781 cactactgca tgagctcacg ttccaggcca tggcgtatga tctgctggac atagagcagg 841 acacatacag gtatgagacc accgggctga gcgaggcgcg ggagaaggcc gtcttgctgg 901 acgaggacga tgacttgtgg gtggagcttc gccacatgca tatcgcagat gtgtccaaga 961 aggtcacgga gctcctgagg accttctgtg agagcaagag gctgaccacg gacaaggcga 1021 acatcaaaga cctatcccag atcctgaaaa agatgccgca gtaccagaag gagctgaata 1081 agtattctac gcacctgcat ctagcagatg attgtatgaa gcacttcaag ggctcggtgg 1141 agaagctgtg tagtgtggag caggacctgg ccatgggctc cgacgcagag ggggagaaga 1201 tcaaggactc catgaagctg atcgttccgg tgctgctgga cgcggcggtg cccgcctacg 1261 acaagatccg ggtcctgctg ctctacatcc tccttcggaa tggtgtgagt gaggagaacc 1321 tggccaagct gatccagcat gccaatgtac aggcgcacag cagcctcatc cgtaacctgg 1381 agcagctggg aggcactgtc accaaccccg ggggctcggg gacctccagc cggctggagc 1441 cgagagaacg catggagccc acctatcagc tgtcccgctg gaccccggtc atcaaggatg 1501 taatggagga cgccgtggag gaccggctgg acaggaacct gtggcccttc gtatccgacc 1561 ccgcccccac ggccagctcc caggccgctg tcagtgcccg cttcggtcac tggcacaaga 1621 acaaggctgg catagaagcc cgggcgggcc cccggctcat cgtgtatgtc atgggcggtg 1681 tggccatgtc agagatgagg gccgcctacg aggtgaccag ggccaccgag ggcaagtggg 1741 aggtgctcat tggctcctca cacatcctca ccccgacccg cttcctggat gacctgaagg 1801 cactggacaa gaagctggag gacattgccc gccctgccca actttcttgt acaaagtggt 1861 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc 1921 taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a