Construct: ORF ccsbBroad304_13971
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018120.1_s304c1, BRDN0000399525
- Derived from:
- ccsbBroadEn_13971
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSP90B1 (7184)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13971
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7184 | HSP90B1 | heat shock protein 90 beta ... | NM_003299.3 | 39% | 38.4% | (many diffs) |
| 2 | human | 7190 | HSP90B2P | heat shock protein 90 beta ... | NR_073383.1 | 32.1% | (many diffs) | |
| 3 | mouse | 22027 | Hsp90b1 | heat shock protein 90, beta... | NM_011631.1 | 35.4% | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gnccntgtgg gtgctgggcc tctgctgcgt cctgctgacc ttcgggtcgg 121 tcagagctga cgatgaagtt gatgtggatg gtacagtaga agaggatctg ggtaaaagta 181 gagaaggatc aaggacggat gatgaagtag tacagagaga ggaagaagct attcagttgg 241 atggattaaa tgcatcacaa ataagagaac ttagagagaa gtcggaaaag tttgccttcc 301 aagccgaagt taacagaatg atgaaactta tcatcaattc attgtataaa aataaagaga 361 ttttcctgag agaactgatt tcaaatgctt ctgatgcttt agataagata aggctaatat 421 cactgactga tgaaaatgct ctttctggaa atgaggaact aacagtcaaa attaagtgtg 481 ataaggagaa gaacctgctg catgtcacag acaccggtgt aggaatgacc agagaagagt 541 tggttaaaaa ccttggtacc atagccaaat ctgggacaag cgagttttta aacaaaatga 601 ctgaagcaca ggaagatggc cagtcaactt ctgaattgat tggccagttt ggtgtcggtt 661 tctattccgc cttccttgta gcagataagg ttattgtcac ttcaaaacac aacaacgata 721 cccagcacat ctgggagtct gactccaatg aattttctgt aattgctgac ccaagaggaa 781 acactctagg acggggaacg acaattaccc ttgtcttaaa agaagaagca tctgattacc 841 ttgaattgga tacaattaaa aatctcgtca aaaaatattc acagttcata aactttccta 901 tttatgtatg gagcagcaag actgaaactg ttgaggagcc catggaggaa gaagaagcag 961 ccaaagaaga gaaagaagaa tctgatgatg aagctgcagc aagacgaaga tgcccaactt 1021 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1081 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta