Construct: ORF ccsbBroad304_14061

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018102.1_s304c1, BRDN0000403809
Derived from:
ccsbBroadEn_14061
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIF3A (11127)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14061
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 11127 KIF3A kinesin family member 3A NM_001300792.2 99.8% 99.4% (many diffs)
2 human 11127 KIF3A kinesin family member 3A NM_007054.7 99.4% 99.1% (many diffs)
3 human 11127 KIF3A kinesin family member 3A XM_017008995.2 97.9% 97% (many diffs)
4 human 11127 KIF3A kinesin family member 3A XM_017008996.2 97.5% 96.7% (many diffs)
5 human 11127 KIF3A kinesin family member 3A NM_001300791.2 96.5% 96% (many diffs)
6 human 11127 KIF3A kinesin family member 3A XM_006714526.4 94.7% 93.6% (many diffs)
7 mouse 16568 Kif3a kinesin family member 3A NM_008443.4 87.6% 97.1% (many diffs)
8 mouse 16568 Kif3a kinesin family member 3A NM_001290805.1 87.2% 96.8% (many diffs)
9 mouse 16568 Kif3a kinesin family member 3A NM_001290806.1 86.7% 96.1% (many diffs)
10 mouse 16568 Kif3a kinesin family member 3A XM_006532326.3 85% 93.9% (many diffs)
11 mouse 16568 Kif3a kinesin family member 3A XM_006532325.3 84.7% 93.8% (many diffs)
12 mouse 16568 Kif3a kinesin family member 3A XM_006532328.3 79.6% 88.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2172
ORF length:
2106
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc gatcaataaa tcagagaagc cagaaagctg cgataatgtg aaggttgttg
121ttaggtgccg gcccctcaat gagagagaga aatcaatgtg ctacaaacag gctgtcagtg
181tggatgagat gaggggaact atcactgtac ataagactga ttcttccaat gaacctccaa
241agacatttac ttttgatact gtttttggac cagagagtaa acaacttgat gtttataact
301taactgcaag acctattatt gattctgtac ttgaaggcta caatgggact atttttgcat
361atggacaaac cggaacaggc aaaactttta ccatggaagg tgttcgagct attcctgaac
421ttagaggaat aattcccaat tcatttgctc acatatttgg tcatattgca aaagcggagg
481gtgatacaag atttttggtt cgagtgtctt atttgggaat atataatgaa gaagttcgtg
541accttttggg caaggatcag acacaaaggt tagaggttat agaaagacct gatgtgggag
601tttatatcaa agatttatca gcttatgtgg taaataatgc tgatgatatg gatagaatta
661tgacgctagg ccacaaaaat cgttctgttg gtgcaactaa tatgaacgaa catagttccc
721gttcccatgc catctttaca attactatag aatgcagtga aaaaggcatt gatggtaaca
781tgcatgtcag gatggggaag ctccatcttg tagatcttgc tggttcagaa agacaggcaa
841aaactggagc tactggacag cgcctaaagg aagctacaaa aatcaatctt tcactttcca
901cccttggtaa tgtaatttct gccttggttg atggaaaaag cactcatgtg ccttatcgta
961actctaaact gactcgtctt cttcaggatt ccttaggagg aaattcaaaa accatgatgt
1021gtgcaaatat tgggccagca gattacaatt atgatgaaac tatcagtaca ttacggtatg
1081ccaatcgtgc taagaatatt aaaaataaag ctagaattaa tgaagatcca aaggatgctt
1141tgctgcgtca gttccagaaa gaaatagaag aactgaaaaa gaagcttgaa gaaggagaag
1201aaatatcagg ctctgatatc agtgggtcag aggaagatga tgatgaagag ggtgaggttg
1261gagaagatgg agagaaaagg aaaaaaagaa gggatcaaac aggaaaaaag aaagtctccc
1321cagacaagat gattgaaatg caagcaaaaa ttgatgagga gagaaaagca cttgaaacaa
1381agctcgacat ggaagaagaa gaaagaaaca aggctagagc tgaattagag aaacgggaaa
1441aagatcttct taaagcccaa caagagcatc agtctttgct ggaaaaatta tctgccctgg
1501aaaagaaggt aattgttggt ggggttgact tgttggccaa agctgaggaa caagagaaac
1561ttcttgaaga atctaacatg gaactggaag aaaggaggaa aagagcagag caacttcgca
1621gagaacttga ggaaaaagag caagaacgct tggatattga agaaaaatat accagtttgc
1681aagaggaagc acagggaaag accaagaagt taaagaaagt ttggactatg ctgatggctg
1741caaagtcaga gatggctgat ctccaacaag aacatcagag ggaaattgaa ggcctactgg
1801agaacattcg gcaacttagc cgggagcttc gacttcagat gcttattatt gataacttta
1861tacctcggga ttatcaggaa atgattgaaa actatgtcca ttggaatgaa gacataggag
1921aatggcagct aaaatgtgtt gcttatacag gaaataacat gaggaagcaa accccagtac
1981ctgataaaaa ggagaaagat ccctttgagg tggacctttc tcacgtgtat cttgcctata
2041ctgaggagag tctgcgtcag tctttgatga aactagaaag accacgaact tcaaagggga
2101aagcaaggcc aaagacaggg agaagaaagc gttctgcaaa gcctgaaact gtaattgact
2161ctttactgna gtacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
2221tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
2281caatcaacct ctggatta

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