Construct: ORF ccsbBroad304_14081
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011868.1_s304c1, BRDN0000401920
- Derived from:
- ccsbBroadEn_14081
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPP1R13B (23368)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14081
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | NM_015316.3 | 99.8% | 47.4% | (many diffs) |
| 2 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_017021116.1 | 96.7% | 45.9% | (many diffs) |
| 3 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_017021117.1 | 96.5% | 45.6% | (many diffs) |
| 4 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_005267487.5 | 93.9% | 44.4% | (many diffs) |
| 5 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_011536593.3 | 91.1% | 43% | (many diffs) |
| 6 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XM_017021119.1 | 73.8% | 21.5% | (many diffs) |
| 7 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_001750206.2 | 64.6% | (many diffs) | |
| 8 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_001750204.2 | 60.6% | (many diffs) | |
| 9 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_001750205.2 | 59.4% | (many diffs) | |
| 10 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_245676.4 | 54.1% | (many diffs) | |
| 11 | human | 23368 | PPP1R13B | protein phosphatase 1 regul... | XR_943413.3 | 53.1% | (many diffs) | |
| 12 | mouse | 21981 | Ppp1r13b | protein phosphatase 1, regu... | NM_011625.1 | 85.9% | 43% | (many diffs) |
| 13 | mouse | 21981 | Ppp1r13b | protein phosphatase 1, regu... | XM_006515796.3 | 83.2% | 41.6% | (many diffs) |
| 14 | mouse | 21981 | Ppp1r13b | protein phosphatase 1, regu... | XM_006515797.3 | 82.9% | 41.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1713
- ORF length:
- 1644
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgccgatg atattaactg ttttcttgag caacaatgaa cagattttaa 121 cagaagttcc tataacaccg gaaacaacct gtcgagatgt tgtagaattt tgcaaggaac 181 ctggagaagg cagctgccat ttagctgaag tgtggagggg aaatgaacgt cccataccct 241 ttgatcatat gatgtacgaa catcttcaga aatggggtcc acggagggaa gaagtgaaat 301 ttttccttcg acacgaggac tccccaactg agaacagtga acaaggtggc cgtcagaccc 361 aagagcaacg aactcagaga aatgtaataa atgtacctgg agaaaaacgt actgaaaatg 421 gggttgggaa tccacgtgtt gaacttaccc tctcagagct ccaagatatg gcagctaggc 481 aacagcagca gattgaaaat cagcagcaga tgttggttgc caaggaacag cgtttacatt 541 ttctaaagca acaggagcgc cgtcagcagc agtctatttc tgaaaatgaa aagcttcaga 601 aattgaaaga acgagttgaa gcccaggaga acaagctgaa gaaaattcgt gcaatgagag 661 gacaagtcga ctacagcaaa atcatgaacg gcaatctgtc tgctgaaata gaaaggttca 721 gtgccatgtt ccaggaaaag aagcaggaag tacagactgc aattttaagg gttgatcagc 781 ttagtcagca attggaagat ttaaagaaag gaaaactgaa tgggttccag tcttacaatg 841 gcaaattgac gggaccagcg gcggtggagt taaaaagact gtaccaagaa ctacagattc 901 gtaaccaact taaccaggaa caaaattcaa aacttcagca gcagaaggaa ctcttaaata 961 agcgcaacat ggaggtggcc atgatggaca agcgaatcag tgaactgcgt gaacgtctct 1021 atgggaaaaa aattcagctg aaccgtgtga atggcacgtc atcaccacag tcccctctga 1081 gcacatcggg cagggtcgct gctgtggggc cttatatcca ggttcccagt gccggaagct 1141 ttcctgtgct gggggaccct ataaagcccc agtctctcag tattgcctca aatgctgctc 1201 atggaagatc caaatccgct aatgatggaa actggccaac attaaaacag aattctagct 1261 cttccgtgaa accagtgcag gtggccggtg cagactggaa ggatccgagc gtggaggggt 1321 ctgtcaagca gggcactgtc tccagccagc ctgtgccctt ctcagcactg ggacccacgg 1381 agaagccggg catcgagatt ggtaaagtgc cacctcccat cccgggtgta ggcaagcagc 1441 tgcctccaag ctatgggaca tacccaagtc ctacacctct gggtcctggg tcgacaagct 1501 ccctggaaag gaggaaggaa ggcagcttgc ccaggcccag tgcaggcctg ccaagtcgac 1561 agaggcccac cctgctgccc gccacaggca gcacccccag ccaggctcct cacaacagat 1621 tcagcagagg atttccgtac cgccaagtcc cacgtacccg ccagcgggac cacctgcatt 1681 tccagctggg gacagcaagc ctgaactccc actgacagtg gccattaggc ctttcctggc 1741 tgataaaggg tcaaggccac agtctcccag gaaaggaccc cagacagtga attcaagttc 1801 catatactcc atgtacctcc agcaagccac accacctaag aattaccagc cggcagcaca 1861 cagcgcctta aataagtcag ttaaagcagt gtatggtaag cccgttttac cttcgggttc 1921 aacctctcca tcgccgctgc cgtttcttca cgggtcactg tccacgggca caccacagcc 1981 tcagccacct tcagaaagta ctgagaaaga gcctgagcag gatggccccg ccgcccccgc 2041 agatggcagc accgtggaga gcctgccacg gccactcagc cccaccaagc tcacgcccat 2101 cgtgcattcg ccactgcgct accagagtga tgcagacctg gaggccctcc gcaggaagct 2161 ggccaacgcg ccccggcccc tgaaaaagcg cagctccatc acagagcccg agggccccgg 2221 cgggcccaac atccagaagc tgctgtacca gcgcttcaac accctggccg gtggcatgga 2281 gggcacccct ttctaccagc ctagcccctc ccaggacttc atgggcacct tggccgatgt 2341 ggacaatgga aacaccaatg ccaatggaaa cctggaagag ctcccccctg cccagcccac 2401 agccccactc cccgctgagc ctgccccgtc atcagatgcc aatgataatg agttaccttc 2461 ccccgaacca gaggagctca tctgtcccca aaccacccac caaactgccg agccggcaga 2521 ggacaataac aacaacgtgg ccacggtccc caccacggag cagatcccga gtcctgtggc 2581 tgaggcccca tctccagggg aagagcaggt ccctccagca cctcttcccc ctgccagcca 2641 ccctcctgcc acctccacga acaagcggac caacttgaag aagcccaact cggagcggac 2701 ggggcacggg ctgagagtcc ggtttaaccc cctggcactg ctcctagacg cgtctctgga 2761 aggagagttc gatctggtgc agaggatcat ctatgaggtg gaagatccca gcaagcccaa 2821 cgacgaaggg atcaccccac tgcacaacgc cgtctgcgcc ggccaccatc acatcgtgaa 2881 gttcctgctg gattttggtg tcaacgtgaa tgctgctgat agtgatggat ggacgccgct 2941 gcactgcgct gcctcttgta acagcgttca cctctgcaaa cagctggtgg agagtggtgc 3001 ctccattttt gcctcaacca taagcgacat tgaaactgct gcagacaagt gtgaggagat 3061 ggaggaaggc tacatccagt gctcccagtt tctatatggg gtgcaggaaa agctgggtgt 3121 gatgaacaaa ggtgtggcgt atgctctgtg ggactacgag gcccagaaca gtgacgagct 3181 gtccttccac gaaggggacg ccctcaccat cctgaggcgc aaggacgaaa gcgagactga 3241 gtggtggtgg gctcgccttg gagaccggga gggctatgtg cccaaaaacc tgctggggct 3301 gtatccacgg gtcaaacccc gacagcgaac actcgccttg ccaactttct tgtacaaagt 3361 ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag 3421 cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta