Construct: ORF ccsbBroad304_14081

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011868.1_s304c1, BRDN0000401920
Derived from:
ccsbBroadEn_14081
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PPP1R13B (23368)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14081
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23368 PPP1R13B protein phosphatase 1 regul... NM_015316.3 99.8% 47.4% (many diffs)
2 human 23368 PPP1R13B protein phosphatase 1 regul... XM_017021116.1 96.7% 45.9% (many diffs)
3 human 23368 PPP1R13B protein phosphatase 1 regul... XM_017021117.1 96.5% 45.6% (many diffs)
4 human 23368 PPP1R13B protein phosphatase 1 regul... XM_005267487.5 93.9% 44.4% (many diffs)
5 human 23368 PPP1R13B protein phosphatase 1 regul... XM_011536593.3 91.1% 43% (many diffs)
6 human 23368 PPP1R13B protein phosphatase 1 regul... XM_017021119.1 73.8% 21.5% (many diffs)
7 human 23368 PPP1R13B protein phosphatase 1 regul... XR_001750206.2 64.6% (many diffs)
8 human 23368 PPP1R13B protein phosphatase 1 regul... XR_001750204.2 60.6% (many diffs)
9 human 23368 PPP1R13B protein phosphatase 1 regul... XR_001750205.2 59.4% (many diffs)
10 human 23368 PPP1R13B protein phosphatase 1 regul... XR_245676.4 54.1% (many diffs)
11 human 23368 PPP1R13B protein phosphatase 1 regul... XR_943413.3 53.1% (many diffs)
12 mouse 21981 Ppp1r13b protein phosphatase 1, regu... NM_011625.1 85.9% 43% (many diffs)
13 mouse 21981 Ppp1r13b protein phosphatase 1, regu... XM_006515796.3 83.2% 41.6% (many diffs)
14 mouse 21981 Ppp1r13b protein phosphatase 1, regu... XM_006515797.3 82.9% 41.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1713
ORF length:
1644
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gatgccgatg atattaactg ttttcttgag caacaatgaa cagattttaa
121cagaagttcc tataacaccg gaaacaacct gtcgagatgt tgtagaattt tgcaaggaac
181ctggagaagg cagctgccat ttagctgaag tgtggagggg aaatgaacgt cccataccct
241ttgatcatat gatgtacgaa catcttcaga aatggggtcc acggagggaa gaagtgaaat
301ttttccttcg acacgaggac tccccaactg agaacagtga acaaggtggc cgtcagaccc
361aagagcaacg aactcagaga aatgtaataa atgtacctgg agaaaaacgt actgaaaatg
421gggttgggaa tccacgtgtt gaacttaccc tctcagagct ccaagatatg gcagctaggc
481aacagcagca gattgaaaat cagcagcaga tgttggttgc caaggaacag cgtttacatt
541ttctaaagca acaggagcgc cgtcagcagc agtctatttc tgaaaatgaa aagcttcaga
601aattgaaaga acgagttgaa gcccaggaga acaagctgaa gaaaattcgt gcaatgagag
661gacaagtcga ctacagcaaa atcatgaacg gcaatctgtc tgctgaaata gaaaggttca
721gtgccatgtt ccaggaaaag aagcaggaag tacagactgc aattttaagg gttgatcagc
781ttagtcagca attggaagat ttaaagaaag gaaaactgaa tgggttccag tcttacaatg
841gcaaattgac gggaccagcg gcggtggagt taaaaagact gtaccaagaa ctacagattc
901gtaaccaact taaccaggaa caaaattcaa aacttcagca gcagaaggaa ctcttaaata
961agcgcaacat ggaggtggcc atgatggaca agcgaatcag tgaactgcgt gaacgtctct
1021atgggaaaaa aattcagctg aaccgtgtga atggcacgtc atcaccacag tcccctctga
1081gcacatcggg cagggtcgct gctgtggggc cttatatcca ggttcccagt gccggaagct
1141ttcctgtgct gggggaccct ataaagcccc agtctctcag tattgcctca aatgctgctc
1201atggaagatc caaatccgct aatgatggaa actggccaac attaaaacag aattctagct
1261cttccgtgaa accagtgcag gtggccggtg cagactggaa ggatccgagc gtggaggggt
1321ctgtcaagca gggcactgtc tccagccagc ctgtgccctt ctcagcactg ggacccacgg
1381agaagccggg catcgagatt ggtaaagtgc cacctcccat cccgggtgta ggcaagcagc
1441tgcctccaag ctatgggaca tacccaagtc ctacacctct gggtcctggg tcgacaagct
1501ccctggaaag gaggaaggaa ggcagcttgc ccaggcccag tgcaggcctg ccaagtcgac
1561agaggcccac cctgctgccc gccacaggca gcacccccag ccaggctcct cacaacagat
1621tcagcagagg atttccgtac cgccaagtcc cacgtacccg ccagcgggac cacctgcatt
1681tccagctggg gacagcaagc ctgaactccc actgacagtg gccattaggc ctttcctggc
1741tgataaaggg tcaaggccac agtctcccag gaaaggaccc cagacagtga attcaagttc
1801catatactcc atgtacctcc agcaagccac accacctaag aattaccagc cggcagcaca
1861cagcgcctta aataagtcag ttaaagcagt gtatggtaag cccgttttac cttcgggttc
1921aacctctcca tcgccgctgc cgtttcttca cgggtcactg tccacgggca caccacagcc
1981tcagccacct tcagaaagta ctgagaaaga gcctgagcag gatggccccg ccgcccccgc
2041agatggcagc accgtggaga gcctgccacg gccactcagc cccaccaagc tcacgcccat
2101cgtgcattcg ccactgcgct accagagtga tgcagacctg gaggccctcc gcaggaagct
2161ggccaacgcg ccccggcccc tgaaaaagcg cagctccatc acagagcccg agggccccgg
2221cgggcccaac atccagaagc tgctgtacca gcgcttcaac accctggccg gtggcatgga
2281gggcacccct ttctaccagc ctagcccctc ccaggacttc atgggcacct tggccgatgt
2341ggacaatgga aacaccaatg ccaatggaaa cctggaagag ctcccccctg cccagcccac
2401agccccactc cccgctgagc ctgccccgtc atcagatgcc aatgataatg agttaccttc
2461ccccgaacca gaggagctca tctgtcccca aaccacccac caaactgccg agccggcaga
2521ggacaataac aacaacgtgg ccacggtccc caccacggag cagatcccga gtcctgtggc
2581tgaggcccca tctccagggg aagagcaggt ccctccagca cctcttcccc ctgccagcca
2641ccctcctgcc acctccacga acaagcggac caacttgaag aagcccaact cggagcggac
2701ggggcacggg ctgagagtcc ggtttaaccc cctggcactg ctcctagacg cgtctctgga
2761aggagagttc gatctggtgc agaggatcat ctatgaggtg gaagatccca gcaagcccaa
2821cgacgaaggg atcaccccac tgcacaacgc cgtctgcgcc ggccaccatc acatcgtgaa
2881gttcctgctg gattttggtg tcaacgtgaa tgctgctgat agtgatggat ggacgccgct
2941gcactgcgct gcctcttgta acagcgttca cctctgcaaa cagctggtgg agagtggtgc
3001ctccattttt gcctcaacca taagcgacat tgaaactgct gcagacaagt gtgaggagat
3061ggaggaaggc tacatccagt gctcccagtt tctatatggg gtgcaggaaa agctgggtgt
3121gatgaacaaa ggtgtggcgt atgctctgtg ggactacgag gcccagaaca gtgacgagct
3181gtccttccac gaaggggacg ccctcaccat cctgaggcgc aaggacgaaa gcgagactga
3241gtggtggtgg gctcgccttg gagaccggga gggctatgtg cccaaaaacc tgctggggct
3301gtatccacgg gtcaaacccc gacagcgaac actcgccttg ccaactttct tgtacaaagt
3361ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag
3421cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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