Transcript: Mouse XM_006515797.3

PREDICTED: Mus musculus protein phosphatase 1, regulatory (inhibitor) subunit 13B (Ppp1r13b), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Ppp1r13b (21981)
Length:
4489
CDS:
246..3605

Additional Resources:

NCBI RefSeq record:
XM_006515797.3
NBCI Gene record:
Ppp1r13b (21981)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006515797.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000088300 CCTGTCGAGATGTCGTAGAAT pLKO.1 316 CDS 100% 5.625 7.875 N Ppp1r13b n/a
2 TRCN0000088299 GCACCTTAAATAAGTCAGTTA pLKO.1 2131 CDS 100% 4.950 3.960 N Ppp1r13b n/a
3 TRCN0000088302 CCACCACCATATCGTGAAGTT pLKO.1 3128 CDS 100% 4.950 3.465 N Ppp1r13b n/a
4 TRCN0000088298 CCCTTCTTGGAAAGCCTCAAA pLKO.1 3977 3UTR 100% 4.950 3.465 N Ppp1r13b n/a
5 TRCN0000088301 CCAGTCATACAATGGCAGGTT pLKO.1 995 CDS 100% 2.640 1.848 N Ppp1r13b n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006515797.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02755 pDONR223 100% 83% 88.6% None (many diffs) n/a
2 ccsbBroad304_02755 pLX_304 0% 83% 88.6% V5 (many diffs) n/a
3 TRCN0000477224 ACTGCCACTGATAAACTACCGCGT pLX_317 14% 83% 88.6% V5 (many diffs) n/a
4 ccsbBroadEn_14081 pDONR223 100% 82.9% 41.3% None (many diffs) n/a
5 ccsbBroad304_14081 pLX_304 0% 82.9% 41.3% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000467453 GTTTATTAAAGCCAACTTGATCGC pLX_317 10.1% 82.9% 41.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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