Construct: ORF ccsbBroad304_14139
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000407.1_s304c1, BRDN0000403154
- Derived from:
- ccsbBroadEn_14139
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AADAT (51166)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14139
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_001286682.1 | 99.8% | 99% | 320C>A;1279delG |
| 2 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_001286683.1 | 98.9% | 97.9% | 67_68insGTGAGAAACGGG;308C>A;1267delG |
| 3 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_016228.4 | 98.9% | 97.9% | 67_68insGTGAGAAACGGG;308C>A;1267delG |
| 4 | human | 51166 | AADAT | aminoadipate aminotransferase | NM_182662.1 | 98.9% | 97.9% | 67_68insGTGAGAAACGGG;308C>A;1267delG |
| 5 | human | 51166 | AADAT | aminoadipate aminotransferase | XM_024454077.1 | 98.9% | 97.9% | 67_68insGTGAGAAACGGG;308C>A;1267delG |
| 6 | human | 51166 | AADAT | aminoadipate aminotransferase | XM_006714231.2 | 90.6% | 89.7% | (many diffs) |
| 7 | human | 51166 | AADAT | aminoadipate aminotransferase | XM_011532020.2 | 71.3% | 70.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1353
- ORF length:
- 1287
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ttacgcacgg ttcatcacgg cagcgagcgc agccagaaac ccttctccca 121 tccggaccat gagtgagaaa cgggctgaca tattgagcag aggaccaaaa tcgatgatct 181 ccttggctgg tggcttacca aatccaaaca tgtttccttt taagactgcc gtaatcactg 241 tagaaaatgg aaagaccatc caatttggag aagagatgat gaagagagca cttcagtatt 301 ctccgagtgc tggaattcca gagcttttgt cctggctaaa acagttacaa ataaaattgc 361 ataatcctcc taccatccat taccaaccca gtcaaggaca aatggatcta tgtgtcacat 421 ctggcagcca acaaggtctt tgtaaggtgt ttgaaatgat cattaatcct ggagataatg 481 tcctcctaga tgaacctgct tattcaggaa ctcttcaaag tctgcaccca ctgggctgca 541 acattattaa tgttgccagt gatgaaagtg ggattgttcc agattcccta agagacatac 601 tttccagatg gaaaccagaa gatgcaaaga atccccagaa aaacaccccc aaatttcttt 661 atactgttcc aaatggcaac aaccctactg gaaactcatt aaccagtgaa cgcaaaaagg 721 aaatctatga gcttgcaaga aaatatgatt tcctcataat agaagatgat ccttactatt 781 ttctccagtt taacaagttc agggtaccaa catttctttc catggatgtt gatggacgtg 841 tcatcagagc tgactctttt tcaaaaatca tttcctctgg gttgagaata ggatttttaa 901 ctggtccaaa acccttaata gagagagtta ttttacacat acaagtttca acattgcacc 961 ccagcacttt taaccagctc atgatatcac agcttctaca cgaatgggga gaagaaggtt 1021 tcatggctca tgtagacagg gttattgatt tctatagtaa ccagaaggat gcaatactgg 1081 cagctgcaga caagtggtta actggtttgg cagaatggca tgttcctgct gctggaatgt 1141 ttttatggat taaagttaaa ggcattaatg atgtaaaaga actgattgaa gaaaaggccg 1201 ttaagatggg ggtattaatg ctccctggaa atgctttcta cgtcgatagc tcagctccta 1261 gcccttactt gagagcatcc ttctcttcag cttctccaga acagatggat gtggccttcc 1321 aggtattagc acaacttata aaaaatcttt atgcccaact ttcttgtaca aagtggttgg 1381 taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa 1441 ccggtggcgc gttaagtcga caatcaacct ctggatta