Construct: ORF ccsbBroad304_14144
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006826.1_s304c1, BRDN0000461730
- Derived from:
- ccsbBroadEn_14144
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CRIM1 (51232)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14144
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51232 | CRIM1 | cysteine rich transmembrane... | NM_016441.3 | 99.7% | 40.1% | (many diffs) |
| 2 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_011532898.3 | 95.9% | 38.6% | (many diffs) |
| 3 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_005264357.5 | 94.1% | 34.5% | (many diffs) |
| 4 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_017004259.1 | 93.5% | 42.8% | (many diffs) |
| 5 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_017004260.2 | 91.9% | 32.3% | (many diffs) |
| 6 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_011532899.3 | 89.9% | 41% | (many diffs) |
| 7 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_011532901.3 | 89.1% | 41.4% | (many diffs) |
| 8 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_017004258.2 | 88.4% | 31.1% | (many diffs) |
| 9 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_024452945.1 | 84.5% | 24.9% | (many diffs) |
| 10 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_024452946.1 | 84.5% | 24.9% | (many diffs) |
| 11 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_024452947.1 | 84.5% | 24.9% | (many diffs) |
| 12 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_024452948.1 | 84.5% | 24.9% | (many diffs) |
| 13 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_017004261.1 | 68.7% | 8.4% | (many diffs) |
| 14 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XM_017004262.1 | 68.7% | 8.4% | (many diffs) |
| 15 | human | 51232 | CRIM1 | cysteine rich transmembrane... | XR_001738763.2 | 52.8% | (many diffs) | |
| 16 | mouse | 50766 | Crim1 | cysteine rich transmembrane... | NM_015800.3 | 87.5% | 36.4% | (many diffs) |
| 17 | mouse | 50766 | Crim1 | cysteine rich transmembrane... | XM_006524601.3 | 80.4% | 29% | (many diffs) |
| 18 | mouse | 50766 | Crim1 | cysteine rich transmembrane... | XM_006524602.3 | 73.5% | 21.9% | (many diffs) |
| 19 | mouse | 50766 | Crim1 | cysteine rich transmembrane... | XM_006524603.3 | 62.5% | 8.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1407
- ORF length:
- 1338
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtacttggtg gcgggggaca gggggttggc cggctgcggg cacctcctgg 121 tctcgctgct ggggctgctg ctgctgctgg cgcgctccgg cacccgggcg ctggtctgcc 181 tgccctgtga cgagtccaag tgcgaggagc ccaggaactg cccggggagc atcgtgcagg 241 gcgtctgcgg ctgctgctac acgtgcgcca gccagaggaa cgagagctgc ggcggcacct 301 tcgggattta cggaacctgc gaccgggggc tgcgttgtgt catccgcccc ccgctcaatg 361 gcgactccct caccgagtac gaagcgggcg tttgcgaaga tgagaactgg actgatgacc 421 aactgcttgg ttttaaacca tgcaatgaaa accttattgc tggctgcaat ataatcaatg 481 ggaaatgtga atgtaacacc attcgaacct gcagcaatcc ctttgagttt ccaagtcagg 541 atatgtgcct ttcagcttta aagagaattg aagaagagaa gccagattgc tccaaggccc 601 gctgtgaagt ccagttctct ccacgttgtc ctgaagattc tgttctgatc gagggttatg 661 ctcctcctgg ggagtgctgt cccttaccca gccgctgcgt gtgcaacccc gcaggctgtc 721 tgcgcaaagt ctgccagccg ggaaacctga acatactagt gtcaaaagcc tcagggaagc 781 cgggagagtg ctgtgacctc tatgagtgca aaccagtttt cggcgtggac tgcaggactg 841 tggaatgccc tcctgttcag cagaccgcgt gtcccccgga cagctatgaa actcaagtca 901 gactaactgc agatggttgc tgtactttgc caacaagatg cgagtgtctc tctggcttat 961 gtggtttccc cgtgtgtgag gtgggatcca ctccccgcat agtctctcgt ggcgatggga 1021 cacctggaaa gtgctgtgat gtctttgaat gtgttaatga tacaaagcca gcctgcgtat 1081 ttaacaatgt ggaatattat gatggagaca tgtttcgaat ggacaactgt cggttctgtc 1141 gatgccaagg gggcgttgcc atctgcttca ctgcccagtg tggtgagata aactgcgaga 1201 ggtactacgt gcccgaagga gagtgctgcc cagtgtgtga agatccagtg tatcctttta 1261 ataatcccgc tggctgctat gccaatggcc tgatccttgg ccacacngag acnggtggcg 1321 ggnagacgac tgcacattct gccagtgcgt caacggtgaa cgccactgcg ttgcgaccgt 1381 ctgcggacag acctgcacaa accctgtgaa agtgcctggg gagtgttgcc ctgtgtgcga 1441 agaaccaacc atcatcacag ttgatccacc tgcatgtggg gagttatcaa actgcactct 1501 gacagggaag gactgcatta atggtttcaa acgcgatcac aatggttgtc ggacctgtca 1561 gtgcataaac accgaggaac tatgttcaga acgtaaacaa ggctgcacct tgaactgtcc 1621 cttcggtttc cttactgatg cccaaaactg tgagatctgt gagtgccgcc caaggcccaa 1681 gaagtgcaga cccataatct gtgacaagta ttgtccactt ggattgctga agaataagca 1741 cggctgtgac atctgtcgct gtaagaaatg tccagagctc tcatgcagta agatctgccc 1801 cttgggtttc cagcaggaca gtcacggctg tcttatctgc aagtgcagag aggcctctgc 1861 ttcagctggg ccacccatcc tgtcgggcac ttgtctcacc gtggatggtc atcatcataa 1921 aaatgaggag agctggcacg atgggtgccg ggaatgctac tgtctcaatg gacgggaaat 1981 gtgtgccctg atcacctgcc cggtgcctgc ctgtggcaac cccaccattc accctggaca 2041 gtgctgccca tcatgtgcag atgactttgt ggtgcagaag ccagagctca gtactccctc 2101 catttgccac gcccctggag gagaatactt tgtggaagga gaaacgtgga acattgactc 2161 ctgtactcag tgcacctgcc acagcggacg ggtgctgtgt gagacagagg tgtgcccacc 2221 gctgctctgc cagaacccct cacgcaccca ggattcctgc tgcccacagt gtacagatca 2281 accttttcgg ccttccttgt cccgcaataa cagcgtacct aattactgca aaaatgatga 2341 aggggatata ttcctggcag ctgagtcctg gaagcctgac gtttgtacca gctgcatctg 2401 cattgatagc gtaattagct gtttctctga gtcctgccct tctgtatcct gtgaaagacc 2461 tgtcttgaga aaaggccagt gttgtcccta ctgcatagaa gacacaattc caaagaaggt 2521 ggtgtgccac ttcagtggga aggcctatgc cgacgaggag cggtgggacc ttgacagctg 2581 cacccactgc tactgcctgc agggccagac cctctgctcg accgtcagct gcccccctct 2641 gccctgtgtt gagcccatca acgtggaagg aagttgctgc ccaatgtgtc cagaaatgta 2701 tgtcccagaa ccaaccaata tacccattga gaagacaaac catcgaggag aggttgacct 2761 ggaggttccc ctgtggccca cgcctagtga aaatgatatc gtccatctcc ctagagatat 2821 gggtcacctc caggtagatt acagagataa caggctgcac ccaagtgaag attcttcact 2881 ggactccatt gcctcagttg tggttcccat aattatatgc ctctctatta taatagcatt 2941 cctattcatc aatcagaaga aacagtggat accactgctt tgctggtatc gaacaccaac 3001 taagccttct tccttaaata atcagctagt atctgtggac tgcaagaaag gaaccagagt 3061 ccaggtggac agttcccaga gaatgctaag aattgcagaa ccagatgcaa gattcagtgg 3121 cttctacagc atgcaaaaac agaaccatct acaggcagac aatttctacc aaacagtgtt 3181 gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga 3241 ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg 3301 gatta