Construct: ORF ccsbBroad304_14200
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008503.1_s304c1, BRDN0000462404
- Derived from:
- ccsbBroadEn_14200
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAML3 (55534)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14200
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55534 | MAML3 | mastermind like transcripti... | NM_018717.5 | 99.4% | 91.6% | (many diffs) |
| 2 | mouse | 433586 | Maml3 | mastermind like 3 (Drosophila) | NM_001004176.2 | 84.9% | 81.3% | (many diffs) |
| 3 | mouse | 433586 | Maml3 | mastermind like 3 (Drosophila) | XM_006501645.3 | 84.8% | 81.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3306
- ORF length:
- 3237
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggggatttc gcagcccccg ctgctgccgc gaatggcagt agtatttgca 121 tcaacagtag cctgaacagc agcctcggcg gggccgggat cggtgtgaat aatactccca 181 atagtactcc cgctgctccg agtagcaatc acccggcagc cggtggatgc ggcggctccg 241 ggggccccgg cggcggttcg gcggccgttc ccaagcacag caccgtggtg gagcggctcc 301 gccagcgcat cgagggctgc cgtcggcacc acgtcaactg cgagaacagg taccagcagg 361 ctcaggtgga gcagctggag ctggagcgcc gggacaccgt gagcctctac cagcggaccc 421 tggagcagag ggccaagaaa tcgggcgccg gcaccggcaa acagcagcac ccgagcaaac 481 cccagcaaga tgcggaggct gcctcggcgg agcagaggaa ccacacgctg atcatgctac 541 aagagactgt gaaaaggaag ttggaaggag ctcgatcacc acttaatgga gaccagcaga 601 atggtgcttg tgatgggaat ttttctccga ctagcaaacg aattcgaaag gacatttctg 661 cggggatgga agccatcaac aatttgccca gtaacatgcc actgccttca gcttctcctc 721 ttcaccaact tgacctgaaa ccttctttgc ccttgcagaa cagtggaact cacactcctg 781 ggcttctaga agatctaagt aagaatggta ggctccctga gattaaactt cctgtcaacg 841 gttgcagtga cctggaggat agcttcacca tcttgcagag caaagacctc aaacaagaac 901 ctctcgatga ccctacttgc atagacacat cagaaacatc tctttcaaat cagaacaagc 961 tgttctcaga cattaatctg aatgatcagg agtggcaaga attaatagat gaattggcca 1021 acacggttcc tgaggatgac atacaggacc tgttcaacga agactttgaa gagaagaagg 1081 agccagaatt ctcgcagcca gcaactgaga cccctctctc ccaggagagt gcgagcgtga 1141 agagcgaccc ctctcactct cccttcgcac atgtctccat gggatctccc caggcgaggc 1201 cttcttcttc tggtcctccc ttttctactg tctccacggc cactagttta ccttctgttg 1261 ccagcactcc cgcagctcca aaccctgcaa gctcaccagc aaactgtgct gtccagtccc 1321 ctcaaactcc aaaccaagcc cacactccag gccaagctcc acctcggcct ggaaatggtt 1381 atctcctgaa tccggcagca gtgacagtgg ccggttcagc gtcagggcct gtggctgtgc 1441 ccagctctga catgtctcca gcagaacagc tcaaacagat ggctgcacag cagcaacaaa 1501 gggccaaact catgcagcag aagcagcaac agcaacagca gcagcagcag cagcagcagc 1561 aacagcagca gcagcagcag caacagcact caaatcagac ttcaaattgg tctcccttag 1621 gacctccctc tagtccatat ggagcagctt ttactgcaga aaaaccaaat agcccaatga 1681 tgtaccccca agcctttaac aaccaaaacc ctatagtgcc tccaatggca aacaacctgc 1741 agaagacaac aatgaataac tacctccctc agaatcacat gaatatgatc aatcagcagc 1801 caaataactt gggtacaaac tccttaaaca aacagcacaa tattctgact tatggcaaca 1861 ctaaacccct gacccacttc aatgcagacc tgagtcagag gatgacacca ccagtggcca 1921 accccaacaa aaaccccttg atgccgtata tccagcagca gcaacagcag cagcaacagc 1981 aacagcagca gcagcagcag cagcagccac cacctccaca gctccaggcc cccagggcac 2041 acctgagcga agaccagaaa cgcctgcttc tcatgaagca gaaaggagtg atgaatcagc 2101 ccatggctta cgctgcactt ccatcccacg gtcaggagca gcatccagtt ggacttcccc 2161 gaaccacagg ccccatgcag tcctccgtgc ccccaggctc aggtggcatg gtctcaggag 2221 ccagtcccgc aggccccggc ttcctgggca gccagcccca agcagccatc atgaagcaga 2281 tgctcattga tcagcgggcc cagttgatag agcagcagaa gcaacagttc ctgcgggagc 2341 aaaggcagca gcagcagcag cagcagcaga ttttggcgga acagcagttg cagcaatcac 2401 atctaccccg gcagcacctc cagccacagc ggaatccata cccagtgcag caggtcaatc 2461 agtttcaagg ttctccccag gatatagcag ccgtaagaag ccaagcagcc ctccagagca 2521 tgcgaacgtc acggctgatg gcacagaacg caggcatgat gggaatagga ccctcccaga 2581 accctgggac gatggccacc gcagctgcgc agtcggagat gggactggcc ccttatagca 2641 ccacgcctac cagccaacca ggaatgtaca atatgagcac aggcatgacc caaatgttgc 2701 agcatccaaa ccaaagtggc atgagcatca cacataacca agcccaggga ccgaggcaac 2761 ctgcctctgg gcagggggtt ggaatggtga gtggctttgg tcagagcatg ctggtgaact 2821 cagccattac ccagcaacat ccacagatga aagggccagt aggccaggcc ttgcctaggc 2881 cccaagcccc tccaaggctg cagagcctta tgggaacagt ccagcaagga gcacaaagct 2941 ggcaacagag gagcttgcag ggcatgcctg ggaggactag tggagaattg ggaccattca 3001 acaatggcgc cagctaccct cttcaagctg ggcagccgag actgaccaag cagcacttcc 3061 cacagggact gagccagtca gtcgtggatg ctaacacggg cacagtgagg accctcaacc 3121 cagctgccat gggtcggcag atgatgccat cgctcccggg gcagcaaggc accagccagg 3181 cgatggccaa tggtcatgtc tggcctgagc cagggagtcc caggcatgcc agcgttcagc 3241 cagcccccag cacagcagca gatacccagt ggcagctttg ctccaagcag ccagagccaa 3301 gcctatgagc ggaatgcccc tcaggacgtg tcatacaatt acagtggcga cggagctggg 3361 ggttccttcc ctggcctccc ggacggtgca gaccttgtgg actccatcat caaaggcggg 3421 ccaggggacg agtggatgca ggagcttgat gaattgtttg gtaacccctt gccaactttc 3481 ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag 3541 taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta