Construct: ORF ccsbBroad304_14228
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008852.1_s304c1, BRDN0000462421
- Derived from:
- ccsbBroadEn_14228
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ADGRG6 (57211)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14228
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_001032395.2 | 99.8% | 33.9% | (many diffs) |
| 2 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_006715518.2 | 99.7% | 33.9% | (many diffs) |
| 3 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_198569.3 | 97.6% | 33.1% | (many diffs) |
| 4 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_006715516.2 | 97.5% | 33.1% | (many diffs) |
| 5 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_006715517.2 | 97.5% | 33% | (many diffs) |
| 6 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_011535964.1 | 97.4% | 33% | (many diffs) |
| 7 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_001032394.2 | 95% | 34.7% | (many diffs) |
| 8 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | NM_020455.5 | 92.9% | 33.9% | (many diffs) |
| 9 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_005267061.3 | 92.8% | 33.9% | (many diffs) |
| 10 | human | 57211 | ADGRG6 | adhesion G protein-coupled ... | XM_017011085.1 | 86.6% | 37.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 67
- ORF end:
- 1336
- ORF length:
- 1269
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggccatga tgtttcgctc agatcgaatg tggagctgcc attggaaatg gaagcccagt 121 cctctcctgt tcttatttgc tttatatatc atgtgtgttc ctcactcagt gtggggatgt 181 gccaactgcc gagtggtttt gtccaaccct tctgggacct ttacttctcc atgctaccct 241 aacgactacc caaacagcca ggcttgcatg tggacgctcc gagcccccac cggttatatc 301 attcagataa catttaacga ctttgacatt gaagaagctc ccaattgcat ttatgactca 361 ttatcccttg ataatggaga gagccagact aaattttgtg gagcaactgc caaaggccta 421 tcatttaact caagtgcgaa tgagatgcat gtgtcctttt caagtgactt tagcatccag 481 aagaaaggtt tcaatgccag ctacatcaga gttgccgtgt ccttaaggaa tcaaaaggtc 541 attttacccc agacatcaga tgcttaccag gtatctgttg caaaaagcat ctctattcca 601 gagctcagtg ctttcacact ctgctttgaa gcaaccaaag ttggccatga agacagtgat 661 tggacagctt tctcctactc aaatgcatcc ttcacacaat tgctcagttt tggaaaggcc 721 aagagtggct actttctatc catttctgat tcacaatgtt tgttgaataa tgcattacct 781 gtcaaagaaa aagaagacat ttttgcagaa agctttgaac agctctgcct tgtttggaat 841 aattctttgg gctctattgg tgtaaatttc aaaagaaact atgaaacagt tccatgtgat 901 tctaccatta gtaaagttat tcctgggaat gggaaattgt tgttgggctc caatcaaaat 961 gaaattgtct ctctaaaagg ggacatttat aactttcgac tttggaattt taccatgaat 1021 gccaaaatcc tctccaacct cagctgtaat gtgaaaggga atgtagtcga ctggcaaaat 1081 gacttctgga atatcccaaa cctagctctg aaagctgaaa gcaacctaag ctgtggttcc 1141 tacctgatcc cgctcccagc agcagaactg gccagctgtg cagacctggg gaccctctgt 1201 caagatggaa ttatctatag aatatccgta gtgattcaga acatccttcg tcaccctgag 1261 gtaaaagtac agagcaaggt ggcagaatgg ctcaattcaa ccttccaaaa attggaacta 1321 cacggtttat gtcgttaata tcagttttca cctgagtgct ggagaggaca agattaaagt 1381 caagagaagc cttgaggatg agccaaggtt ggtgctttgg gcccttctag tttacaatgc 1441 taccaacaat actaatttag aaggaaaaat cattcagcag aagctcctaa aaaataatga 1501 gtccttggat gaaggcttga ggctacatac agtgaatgtg agacaactgg gtcattgtct 1561 tgccatggag gaacccaaag gctactactg gccatctatc caaccttctg aatacgttct 1621 tccttgtcca gacaagcctg gcttttctgc ttctcggata tgtttttaca atgctaccaa 1681 cccattggta acctactggg gacctgttga tatctccaac tgtttaaaag aagcaaatga 1741 agttgctaac cagattttaa atttaactgc tgatgggcag aacttaacct cagccaatat 1801 taccaacatt gtggaacagg tcaaaagaat tgtgaataaa gaagaaaaca ttgatataac 1861 acttggctca actctaatga atatattttc taatatctta agcagttcag acagtgactt 1921 gcttgagtca tcttctgaag ctttaaaaac aattgatgaa ttggccttca agatagacct 1981 aaatagcaca tcacatgtga atattacaac tcggaacttg gctctcagcg tatcatccct 2041 gttaccaggg acaaatgcaa tttcaaattt tagcattggt cttccaagca ataatgaatc 2101 gtatttccag atggattttg agagtggaca agtggatcca ctggcatctg taattttgcc 2161 tccaaactta cttgagaatt taagtccaga agattctgta ttagttagaa gagcacagtt 2221 tactttcttc aacaaaactg gacttttcca ggatgtagga ccccaaagaa aaactttagt 2281 gagttatgtg atggcgtgca gtattggaaa cattactatc cagaatctga aggatcctgt 2341 tcaaataaaa atcaaacata caagaactca ggaagtgcat catcccatct gtgccttctg 2401 ggatctgaac aaaaacaaaa gttttggagg atggaacacg tcaggatgtg ttgcacacag 2461 agattcagat gcaagtgaga cagtctgcct gtgtaaccac ttcacacact ttggagttct 2521 gatggacctt ccaagaagtg cctcacagtt agatgcaaga aacactaaag tcctcacttt 2581 catcagctat attgggtgtg gaatatctgc tattttttca gcagcaactc tcctgacata 2641 tgttgctttt gagaaattgc gaagggatta tccctccaaa atcttgatga acctgagcac 2701 agccccgctg ttcctgaatc tcctcttcct cctagatggc tggatcacct ccttcaatgt 2761 ggatggactt tgcattgctg ttgcagtcct gttgcatttc ttccttctgg caacctttac 2821 ctggatgggg ctagaagcaa ttcacatgta cattgctcta gttaaagtat ttaacactta 2881 cattcgccga tacattctaa aattctgcat cattggctgg ggtttgcctg ccttagtggt 2941 gtcagttgtt ctagcgagca gaaacaacaa tgaagtctat ggaaaagaaa gttatgggaa 3001 agaaaaaggt gatgaattct gttggattca agatccagtc atattttatg tgacctgtgc 3061 tgggtatttt ggagtcatgt tttttctgaa cattgccatg ttcattgtgg taatggtgca 3121 gatctgtggg aggaatggca agagaagcaa ccggaccctg agagaagaag tgttaaggaa 3181 cctgcgcagt gtggttagct tgacctttct gttgggcatg acatggggtt ttgcattctt 3241 tgcctgggga cccttaaata tccccttcat gtacctcttc tccatcttca attcattaca 3301 aggcttattt atattcatct tccactgtgc tatgaaggag aatgttcaga aacagtggcg 3361 gcggcatctc tgctgtggta gatttcggtt agcagataac tcagattgga gtaagacagc 3421 taccaatatc atcaagaaaa gttctgataa tctaggaaaa tctttgtctt caagctccat 3481 tggttccaac tcaacctatc ttacatccaa atctaaatcc agctctacca cctatttcaa 3541 aaggaatagc cacacagaca gtgcttccat ggacaagtcc ttgtcaaaac tggcccatgc 3601 tgatggagat caaacatcaa tcatccctgt ccatcaggtc attgataagg tcaagggtta 3661 ttgcaatgct cattcagaca acttctataa aaatattatc atgtcagaca ccttcagcca 3721 acagcacaaa gtttttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 3781 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 3841 cgacaatcaa cctctggatt a