Construct: ORF ccsbBroad304_14287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005425.1_s304c1, BRDN0000461542
- Derived from:
- ccsbBroadEn_14287
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BHLHB9 (80823)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14287
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_001142524.2 | 99.8% | 98.9% | 705C>A;1621delA |
| 2 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_001142525.2 | 99.8% | 98.9% | 705C>A;1621delA |
| 3 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_001142526.2 | 99.8% | 98.9% | 705C>A;1621delA |
| 4 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_001142527.2 | 99.8% | 98.9% | 705C>A;1621delA |
| 5 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_001142528.2 | 99.8% | 98.9% | 705C>A;1621delA |
| 6 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_001142529.1 | 99.8% | 98.9% | 705C>A;1621delA |
| 7 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_001142530.1 | 99.8% | 98.9% | 705C>A;1621delA |
| 8 | human | 100528062 | ARMCX5-GPRASP2 | ARMCX5-GPRASP2 readthrough | NM_001350268.2 | 99.8% | 98.9% | 705C>A;1621delA |
| 9 | human | 100528062 | ARMCX5-GPRASP2 | ARMCX5-GPRASP2 readthrough | NM_001350269.2 | 99.8% | 98.9% | 705C>A;1621delA |
| 10 | human | 100528062 | ARMCX5-GPRASP2 | ARMCX5-GPRASP2 readthrough | NM_001350270.1 | 99.8% | 98.9% | 705C>A;1621delA |
| 11 | human | 80823 | BHLHB9 | basic helix-loop-helix fami... | NM_030639.3 | 99.8% | 98.9% | 705C>A;1621delA |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1707
- ORF length:
- 1641
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tgggactaag aataagacaa gagcccaggc caaaactgaa aaaaaggctg 121 ctatacaagc taaagctgga gcagagaggg aggctactgg tgttgttagg cctgtagcca 181 agaccagggc caaagcaaaa gccaagacag ggtctaagac agatgcagta gcagagatga 241 aggcagtgtc taagaacaag gttgttgctg agacgaagga aggagctctg tcagagccta 301 agactctggg caaagccatg ggagatttca ctcccaaggc tgggaatgag tccaccagct 361 ccacatgtaa aaatgaggct ggtactgatg cctggttctg ggctggggaa gaggccacta 421 tcaattcctg gttctggaat ggagaagagg ctggtaatag tttcagcact aagaatgata 481 aacctgaaat tggtgcccag gtctgtgctg aggagttgga acctgcggct ggggccgatt 541 gcaaacctag gtcaggggct gaggaggagg aggaagagaa tgttattggg aactggtttt 601 gggaaggaga tgatactagt tttgacccta atcctaaacc tgtgagcagg atagttaagc 661 ctcagcctgt gtatgaaatt aatgaaaaaa ataggcccaa ggactggtct gaggtaacta 721 tctggcccaa tgcccctgct gtaactccag ctgtgttagg atttagatca caggcaccat 781 ctgaggcaag ccctccttca tatattgttc tggcctccgc tgaagaaaat gcctgttctt 841 tgcctgtggc aacagcttgc cgcccttcta ggaacactcg ctcatgctca cagcctatcc 901 ctgagtgtcg ttttgattct gacccctgca tccagaccat agatgagatt agacgtcaaa 961 tcaggatcag ggaggtaaat gggattaagc catttgcttg tccttgcaaa atggaatgct 1021 atatggattc tgaggaattt gaaaaacttg ttagcttact taagtcaact actgatcctc 1081 ttattcataa aatagcacgg attgcaatgg gtgtccataa tgttcaccca tttgcccaag 1141 agtttattaa cgaagtaggt gtagtgacac ttattgaaag cttgctcagt tttccttccc 1201 ctgaaatgag aaaaaagact gtaattactc tgaatcctcc ttctggggat gaaagacaac 1261 gcaaaattga attacatgtt aagcatatgt gtaaagaaac catgtcattt cctttgaact 1321 caccgggaca gcaatctgga ttaaagatac taggacaact gactactgat tttgtccatc 1381 actacattgt tgccaattac ttttcagagc ttttccattt gctgtcctca ggaaattgca 1441 aaaccagaaa tcttgttttg aaactacttt taaatatgtc tgaaaatcca actgcagcca 1501 gagacatgat caatatgaag gcattggcag cattaaaact catctttaac cagaaagagg 1561 caaaagccaa tcttgttagt ggtgtggcca tatttattaa cataaaggag catatcagaa 1621 aaggctcaat tgtagttgtt gatcacttga gttataatac actcatggcc attttcaggg 1681 aagttaagag attattgaaa caatgtaccc aactttcttg tacaaagtgg ttggtaagcc 1741 tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg 1801 gcgcgttaag tcgacaatca acctctggat ta