Transcript: Human NM_001350270.1

Homo sapiens ARMCX5-GPRASP2 readthrough (ARMCX5-GPRASP2), transcript variant 8, mRNA.

Source:
NCBI, updated 2018-12-22
Taxon:
Homo sapiens (human)
Gene:
ARMCX5-GPRASP2 (100528062)
Length:
5578
CDS:
1034..2677

Additional Resources:

NCBI RefSeq record:
NM_001350270.1
NBCI Gene record:
ARMCX5-GPRASP2 (100528062)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001350270.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430355 GGAGTTCCTATAGGCTATTTA pLKO_005 3128 3UTR 100% 15.000 7.500 Y BHLHB9 n/a
2 TRCN0000418281 TTAGTGGTGTGGCCATATTTA pLKO_005 2544 CDS 100% 15.000 7.500 Y BHLHB9 n/a
3 TRCN0000168798 GCCAGGTGTTACAATTCTTAA pLKO.1 3670 3UTR 100% 13.200 6.600 Y BHLHB9 n/a
4 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 5005 3UTR 100% 4.950 2.475 Y n/a
5 TRCN0000168215 CTTTAACCAGAAAGAGGCAAA pLKO.1 2512 CDS 100% 4.050 2.025 Y BHLHB9 n/a
6 TRCN0000172810 GAGGTAACTATCTGGCCCAAT pLKO.1 1679 CDS 100% 4.050 2.025 Y BHLHB9 n/a
7 TRCN0000168686 GCAGTGTCTAAGAACAAGGTT pLKO.1 1211 CDS 100% 3.000 1.500 Y BHLHB9 n/a
8 TRCN0000168685 GCCCAAGAGTTTATTAACGAA pLKO.1 2102 CDS 100% 3.000 1.500 Y BHLHB9 n/a
9 TRCN0000168148 CCAATGAGAATTAGAGAGGTT pLKO.1 3473 3UTR 100% 2.640 1.320 Y BHLHB9 n/a
10 TRCN0000172742 GAGACAGAGTCTTGCTCTGTT pLKO.1 4826 3UTR 100% 0.495 0.248 Y C11orf44 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001350270.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14287 pDONR223 100% 99.8% 98.9% None 705C>A;1621delA n/a
2 ccsbBroad304_14287 pLX_304 0% 99.8% 98.9% V5 (not translated due to frame shift) 705C>A;1621delA n/a
3 TRCN0000477311 TTCATTTTTGCGGCCAACGCACTA pLX_317 4.1% 99.8% 98.9% V5 (not translated due to frame shift) 705C>A;1621delA n/a
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