Construct: ORF ccsbBroad304_14290

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002763.1_s304c1, BRDN0000410124
Derived from:
ccsbBroadEn_14290
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC38A1 (81539)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14290
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81539 SLC38A1 solute carrier family 38 me... NM_001077484.2 99.7% 99.5% 10T>G;1453_1454delGAinsCG
2 human 81539 SLC38A1 solute carrier family 38 me... NM_001278387.2 99.7% 99.5% 10T>G;1453_1454delGAinsCG
3 human 81539 SLC38A1 solute carrier family 38 me... NM_001278388.2 99.7% 99.5% 10T>G;1453_1454delGAinsCG
4 human 81539 SLC38A1 solute carrier family 38 me... NM_001278389.2 99.7% 99.5% 10T>G;1453_1454delGAinsCG
5 human 81539 SLC38A1 solute carrier family 38 me... NM_030674.4 99.7% 99.5% 10T>G;1453_1454delGAinsCG
6 human 81539 SLC38A1 solute carrier family 38 me... XM_011538786.2 94.4% 91.9% (many diffs)
7 human 81539 SLC38A1 solute carrier family 38 me... XM_011538787.2 93.4% 93% (many diffs)
8 human 81539 SLC38A1 solute carrier family 38 me... XM_017019990.1 93.4% 93% (many diffs)
9 human 81539 SLC38A1 solute carrier family 38 me... XM_011538784.2 93% 91.6% (many diffs)
10 human 81539 SLC38A1 solute carrier family 38 me... NM_001278390.1 92.7% 92% (many diffs)
11 mouse 105727 Slc38a1 solute carrier family 38, m... NM_001166456.1 84.2% 92.6% (many diffs)
12 mouse 105727 Slc38a1 solute carrier family 38, m... NM_001166458.1 84.2% 92.6% (many diffs)
13 mouse 105727 Slc38a1 solute carrier family 38, m... NM_134086.4 84.2% 92.6% (many diffs)
14 mouse 105727 Slc38a1 solute carrier family 38, m... XM_006520235.3 84.2% 92.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1527
ORF length:
1461
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat gcatgtcaaa agtggactcg aattaactga gttgcaaaac atgacagtgc
121ccgaggatga taacattagc aatgactcca atgatttcac cgaagtagaa aatggtcaga
181taaatagcaa gtttatttct gatcgtgaaa gtagaagaag tctcacaaac agccatttgg
241aaaaaaagaa gtgtgatgag tatattccag gtacaacctc cttaggcatg tctgttttta
301acctaagcaa cgccattatg ggcagtggga ttttgggact cgcctttgcc ctggcaaaca
361ctggaatcct actttttctg gtacttttga cttcagtgac attgctgtct atatattcaa
421taaacctcct attgatctgt tcaaaagaaa caggctgcat ggtgtatgaa aagctggggg
481aacaagtctt tggcaccaca gggaagttcg taatctttgg agccacctct ctacagaaca
541ctggagcaat gctgagctac ctcttcatcg taaaaaatga actaccctct gccataaagt
601ttctaatggg aaaggaagag acattttcag cctggtacgt ggatggccgc gttctggtgg
661tgatagttac ctttggcata attctccctc tgtgtctctt gaagaactta gggtatcttg
721gctatactag tggattttcc ttgagctgta tggttttttt cctaattgtg gttatttaca
781agaaatttca aattccctgc attgttccag agctaaattc aacaataagt gctaattcaa
841caaatgctga cacgtgtacg ccaaaatatg ttaccttcaa ttcaaagacc gtgtatgctt
901tacccaccat tgcatttgca tttgtttgcc acccgtcagt cctgccaatt tacagtgagc
961ttaaagaccg atcacagaaa aaaatgcaga tggtttcaaa catctccttt ttcgccatgt
1021ttgttatgta cttcttgact gccatttttg gctacttgac attctatgac aacgtgcagt
1081ccgacctcct tcacaaatat cagagtaaag atgacattct catcctgaca gtgcggctgg
1141ctgtcattgt tgctgtgatc ctcacagtgc cggtgttatt tttcacggtt cgttcatctt
1201tatttgaact ggctaagaaa acaaagttta atttatgtcg tcataccgtg gttacctgca
1261tactcttggt tgttatcaac ttgttggtga tcttcatacc ctccatgaag gatatttttg
1321gagtcgtagg agttacatct gctaacatgc ttattttcat tcttccttca tctctttatt
1381taaaaatcac agaccaggat ggagataaag gaactcaaag aatttgggct gcccttttct
1441tgggcctggg ggtgttgttc tccttggtca gcattccctt ggtcatctat gactgggcct
1501gctcatcgag tagtgaccga ggccactgcc caactttctt gtacaaagtg gttggtaagc
1561ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
1621ggcgcgttaa gtcgacaatc aacctctgga tta

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