Construct: ORF ccsbBroad304_14304

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009459.1_s304c1, BRDN0000410137
Derived from:
ccsbBroadEn_14304
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SLC41A2 (84102)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14304
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84102 SLC41A2 solute carrier family 41 me... NM_001352169.1 85.4% 85.1% 1_249del;1712delT
2 human 84102 SLC41A2 solute carrier family 41 me... NM_001352170.2 85.4% 85.1% 1_249del;1712delT
3 human 84102 SLC41A2 solute carrier family 41 me... NM_001352171.2 85.4% 85.1% 1_249del;1712delT
4 human 84102 SLC41A2 solute carrier family 41 me... NM_001352172.2 85.4% 85.1% 1_249del;1712delT
5 human 84102 SLC41A2 solute carrier family 41 me... NM_032148.5 85.4% 85.1% 1_249del;1712delT
6 human 84102 SLC41A2 solute carrier family 41 me... XM_005269179.1 85.4% 85.1% 1_249del;1712delT
7 human 84102 SLC41A2 solute carrier family 41 me... XM_011538808.1 85.4% 85.1% 1_249del;1712delT
8 human 84102 SLC41A2 solute carrier family 41 me... XM_011538810.2 85.4% 85.1% 1_249del;1712delT
9 human 84102 SLC41A2 solute carrier family 41 me... XM_011538811.2 85.4% 85.1% 1_249del;1712delT
10 human 84102 SLC41A2 solute carrier family 41 me... XM_017020013.1 85.4% 85.1% 1_249del;1712delT
11 human 84102 SLC41A2 solute carrier family 41 me... XM_024449219.1 85.4% 85.1% 1_249del;1712delT
12 human 84102 SLC41A2 solute carrier family 41 me... XM_017020014.1 76.5% 75.6% (many diffs)
13 human 84102 SLC41A2 solute carrier family 41 me... XM_017020016.2 73.8% 73.4% 0_1ins384;1079delT
14 human 84102 SLC41A2 solute carrier family 41 me... XM_017020017.1 72.9% 72% (many diffs)
15 human 84102 SLC41A2 solute carrier family 41 me... XM_011538813.2 70.7% 70.4% 0_1ins429;1034delT
16 human 84102 SLC41A2 solute carrier family 41 me... XM_017020015.1 54.4% 54.1% (many diffs)
17 mouse 338365 Slc41a2 solute carrier family 41, m... NM_177388.3 72.4% 78.3% (many diffs)
18 mouse 338365 Slc41a2 solute carrier family 41, m... XM_006513811.3 72.4% 78.3% (many diffs)
19 mouse 338365 Slc41a2 solute carrier family 41, m... XM_011243498.2 72.4% 78.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1536
ORF length:
1470
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gtatcacagt ttctcagagc agtcttttca tgccaataat gggcacgcat
121catcaagctg cagccaaaag tatgatgact atgccaatta taattactgt gatggaaggg
181agacttcaga aaccactgcc atgttacaag atgaagatat atctagtgat ggtgatgaag
241atgctattgt agaagtgacc ccaaaattac caaaggaatc cagtggcatc atggcattgc
301aaatacttgt gccctttttg ctagctggtt ttggaacagt ttcagctggc atggtactgg
361atatagtaca gcactgggag gtgttcagaa aagttacaga agttttcatt ttagtccctg
421cacttcttgg tctcaaaggg aacttggaaa tgacattggc atccagatta tccactgcag
481taaatattgg gaagatggat tcacccattg aaaagtggaa cctaataatt ggcaacttgg
541ctttaaagca ggttcaggca acagtagtgg gttttctagc agctgtggca gcaattatat
601tgggctggat tccagaagga aaatattacc ttgatcattc catacttctg tgctctagca
661gtgtggcaac tgccttcatt gcatctcttc tgcagggaat aataatggtt ggggttatcg
721ttggttcaaa gaagactggt ataaatcctg ataatgttgc tacacccatt gctgctagtt
781ttggcgacct tataactctt gccatattgg cttggataag tcagggctta tactcctgtc
841ttgagaccta ttactacatt tctccattag ttggtgtatt tttcttggct ctaaccccta
901tttggattat aatagctgcc aaacatccag ccacaagaac agttctccac tcaggctggg
961agcctgtcat aacagctatg gttataagta gcattggggg ccttattctg gacacaactg
1021tatcagaccc aaacttggtt gggattgttg tttacacgcc agttattaat ggtattggtg
1081gtaatttggt ggccattcag gctagcagga tttctaccta cctccattta catagcattc
1141caggagaatt gcctgatgaa cccaaaggtt gttactaccc atttagaact ttctttggtc
1201caggagtaaa taataagtct gctcaagttc tactgctttt agtgattcct ggacatttaa
1261ttttcctcta cactattcat ttgatgaaaa gtggtcatac ttctttaact ataatcttca
1321tagtagtgta tttatttggc gctgtgttac aggtatttac cttgctgtgg attgctgact
1381ggatggtcca tcacttctgg aggaaaggaa aggacccgga tagtttctcc atcccctacc
1441taacagcatt gggtgatctg ctcgggacag ctctgttagc cttaagtttt cattttcttt
1501ggcttattgg agatcgagat ggagatgtgg agactaccca actttcttgt acaaagtggt
1561tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc
1621taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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