Construct: ORF ccsbBroad304_14403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016016.1_s304c1, BRDN0000461309
- Derived from:
- ccsbBroadEn_14403
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC35F3 (148641)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14403
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148641 | SLC35F3 | solute carrier family 35 me... | NM_001300845.1 | 99.8% | 99.5% | 692C>G;1261C>N |
| 2 | human | 148641 | SLC35F3 | solute carrier family 35 me... | NM_173508.4 | 83.5% | 81% | (many diffs) |
| 3 | mouse | 210027 | Slc35f3 | solute carrier family 35, m... | NM_175434.3 | 87.3% | 91.4% | (many diffs) |
| 4 | mouse | 210027 | Slc35f3 | solute carrier family 35, m... | XM_011248334.1 | 76.2% | 77% | (many diffs) |
| 5 | mouse | 210027 | Slc35f3 | solute carrier family 35, m... | XM_011248333.2 | 73.3% | 74% | (many diffs) |
| 6 | mouse | 210027 | Slc35f3 | solute carrier family 35, m... | XM_006530805.2 | 65.7% | 67.4% | (many diffs) |
| 7 | mouse | 210027 | Slc35f3 | solute carrier family 35, m... | XM_006530806.3 | 65.7% | 67.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaagcactcg gcccgggtgg ccccgctcag cgcctgcaac agtccggtcc 121 tgacccttac caaagtggaa ggggaggagc gcccccggga ctccccgggc ccggcggagg 181 cccaggcacc ggccggggtg gaggccggcg ggagagcgag tcgccgctgc tggacgtgct 241 cccgggcgca actcaagaag atcttctggg gcgtggcggt cgtgctgtgc gtgtgctcct 301 cgtgggcggg ctccacgcag ctcgccaagc tgaccttcag gaagttcgac gcgcccttca 361 ccctcacgtg gtttgccacc aactggaact ttttattctt cccgttgtac tacgtggggc 421 acgtctgcaa gtccacagag aagcagtctg tgaagcagcg atacagggaa tgctgtcgat 481 tttttggaga caatggcttg actttgaagg tgttttttac caaggcagca ccctttggtg 541 ttctttggac actcacaaac tacctgtact tacatgcaat aaagaaaata aacactacgg 601 atgtctccgt gttgttctgc tgcaacaaag cttttgtgtt cttgctctca tggatcgttc 661 tcagggacag attcatggga gtgaggattg tggccgccat cctcgccatc gctggcattg 721 tgatgatgac ctacgctgat ggcttccaca gccactgcgt catcggcatc gcactggtgg 781 tggcctcagc atcgatgtct gccctctaca aggttttgtt caagctcctc ctgggcagtg 841 ctaagtttgg agaagccgcc ttatttttgt ccatcttggg tgtgtttaac atcctcttca 901 tcacctgcat tcctattatc ctctacttta ccaaagtgga atactggagc tcttttgatg 961 acattccatg gggaaacctt tgtggatttt cagttctttt attgacattc aatattgtat 1021 taaattttgg aattgccgtt acatatccca ctctgatgtc tcttggaatc gtcctcagca 1081 tacctgtgaa tgcagtgatt gatcactaca ccagtcagat cgtcttcaat ggggtccggg 1141 tcatcgccat catcatcatc ggcctgggtt ttctcctcct gctcctgcca gaggagtggg 1201 atgtctggtt gatcaagctg ctcacccgac tcaaagtgag gaagaaggag gagcctgcag 1261 agggcgctgc cgacctgagc tcaggacctc agagcaagaa cagaagagcc cgcccttcct 1321 tcgccngcta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1381 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1441 tcaacctctg gatta