Construct: ORF ccsbBroad304_14428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018195.1_s304c1, BRDN0000401101
- Derived from:
- ccsbBroadEn_14428
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TET3 (200424)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14428
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_011532690.1 | 98.1% | 46% | (many diffs) |
| 2 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_024452747.1 | 98.1% | 46% | (many diffs) |
| 3 | human | 200424 | TET3 | tet methylcytosine dioxygen... | NM_001366022.1 | 54.9% | 25.7% | (many diffs) |
| 4 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_011532687.2 | 54.4% | 25.5% | (many diffs) |
| 5 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_011532686.2 | 52.6% | 24.7% | (many diffs) |
| 6 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_011532685.2 | 52.2% | 24.4% | (many diffs) |
| 7 | human | 200424 | TET3 | tet methylcytosine dioxygen... | NM_001287491.2 | 52.1% | 24.4% | (many diffs) |
| 8 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_011532682.2 | 51.6% | 24.2% | (many diffs) |
| 9 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_011532683.2 | 51.6% | 24.2% | (many diffs) |
| 10 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_011532684.2 | 51.6% | 24.2% | (many diffs) |
| 11 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_017003566.1 | 51.6% | 24.2% | (many diffs) |
| 12 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_024452746.1 | 12.8% | 17.7% | 1_2574del;2593G>A;3267_3268ins2118 |
| 13 | human | 200424 | TET3 | tet methylcytosine dioxygen... | XM_024452745.1 | 12.7% | 17.5% | (many diffs) |
| 14 | mouse | 194388 | Tet3 | tet methylcytosine dioxygen... | NM_183138.2 | 49% | 25.1% | (many diffs) |
| 15 | mouse | 194388 | Tet3 | tet methylcytosine dioxygen... | XM_006505777.3 | 45.8% | 23.4% | (many diffs) |
| 16 | mouse | 194388 | Tet3 | tet methylcytosine dioxygen... | NM_001347313.1 | 45.3% | 23.2% | (many diffs) |
| 17 | mouse | 194388 | Tet3 | tet methylcytosine dioxygen... | XM_006505775.3 | 45.3% | 23.2% | (many diffs) |
| 18 | mouse | 194388 | Tet3 | tet methylcytosine dioxygen... | XM_006505776.3 | 45.3% | 23.2% | (many diffs) |
| 19 | mouse | 194388 | Tet3 | tet methylcytosine dioxygen... | XM_017321465.1 | 45.3% | 23.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1386
- ORF length:
- 1320
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ggagcggtat ggaaagaagg ggaaagccat ccggatcgag aaggtcatct 121 acacggggaa ggagggaaag agctcccgcg gttgccccat tgcaaagtgg gtgatccgca 181 ggcacacgct ggaggagaag ctactctgcc tggtgcggca ccgggcaggc caccactgcc 241 agaacgctgt gatcgtcatc ctcatcctgg cctgggaggg cattccccgt agcctcggag 301 acaccctcta ccaggagctc accgacaccc tccggaagta tgggaacccc accagccgga 361 gatgcggcct caacgatgac cggacctgcg cttgccaagg caaagacccc aacacctgtg 421 gtgcctcctt ctcctttggt tgttcctgga gcatgtactt caacggctgc aagtatgctc 481 ggagcaagac acctcgcaag ttccgcctcg caggggacaa tcccaaagag gaagaagtgc 541 tccggaagag tttccaggac ctggccaccg aagtcgctcc cctgtacaag cgactggccc 601 ctcaggccta tcagaaccag gtgaccaacg aggaaatagc gattgactgc cgtctggggc 661 tgaaggaagg acggcccttc gcgggggtca cggcctgcat ggacttctgt gcccacgccc 721 acaaggacca gcataacctc tacaatgggt gcaccgtggt ctgcaccctg accaaggaag 781 acaatcgctg cgtgggcaag attcccgagg atgagcagct gcatgttctc cccctgtaca 841 agatggccaa cacggatgag tttggtagcg aggagaacca gaatgcaaag gtgggcagcg 901 gagccatcca ggtgctcacc gccttccccc gcgaggtccg acgcctgccc gagcctgcca 961 agtcctgccg ccagcggcag ctggaagcca gaaaggcagc agccgagaag aagaagattc 1021 agaaggagaa gctgagcact ccggagaaga tcaagcagga ggccctggag ctggcgggca 1081 ttacgtcgga cccaggcctg tctctgaagg gtggattgtc ccagcaaggc ctgaagccct 1141 ccctcaaggt ggagccgcag aaccacttca gctccttcaa gtacagcggc aacgcggtgg 1201 tggagagcta ctcggtgctg ggcaactgcc ggccctccga cccttacagc atgaacagcg 1261 tgtactccta ccactcctac tatgcacagc ccagcctgac ctccgtcaat ggcttccact 1321 ccaagtacgc tctcccgtct tttagctact atggctttcc atccagcaac cccgtcttcc 1381 cctcttagtt cctgggtcct ggtgcctggg ggcatagtgg cagcagtggc agttttgaga 1441 agaagccaga cctccacgct ctgcacaaca gcctgagccc ggcctacggt ggtgctgagt 1501 ttgccgagct gcccagccag gctgttccca cagacgccca ccaccccact cctcaccacc 1561 agcagcctgc gtacccaggc cccaaggagt atctgcttcc caaggccccc ctactccact 1621 cagtgtccag ggaccccccc ccctttgccc agagctccaa ctgctacaac agatccatca 1681 agcaagagcc agtagacccg ctgacccagg ctgagcctgt gcccagagac gctggcaaga 1741 tgggcaagac acctctgtcc gaggtgtctc agaatggagg acccagtcac ctttggggac 1801 agtactcagg aggcccaagc atgtccccca agaggactaa cggtgtgggt ggcagctggg 1861 gtgtgttctc gtctggggag agtcctgcca tcgtccctga caagctcagt tcctttgggg 1921 ccagctgcct ggccccttcc cacttcacag atggccagtg ggggctgttc cccggtgagg 1981 ggcagcaggc agcttcccac tctggaggac ggctgcgagg caaaccgtgg agcccctgca 2041 agtttgggaa cagcacctcg gccttggctg ggcccagcct gactgagaag ccgtgggcgc 2101 tgggggcagg ggatttcaac tcggccctga aaggtagtcc tgggttccaa gacaagctgt 2161 ggaaccccat gaaaggagag gagggcagga ttccagccgc aggggccagc cagctggaca 2221 gggcctggca gtcctttggt ctgcccctgg gatccagcga gaagctgttt ggggctctga 2281 agtcagagga gaagctgtgg gaccccttca gcctggagga ggggccggct gaggagcccc 2341 ccagcaaggg agcggtgaag gaggagaagg gcggtggtgg tgcggaggag gaagaggagg 2401 agctgtggtc ggacagtgaa cacaacttcc tggacgagaa catcggcggc gtggccgtgg 2461 ccccagccca cggctccatc ctcatcgagt gtgcccggcg ggagctgcac gccaccacgc 2521 cgcttaagaa gcccaaccgc tgccacccca cccgcatctc gctggtcttc taccagcaca 2581 agaacctcaa ccagcccaac cacgggctgg ccctctggga agccaagatg aagcagctgg 2641 cggagagggc acgggcacgg caggaggagg ctgcccggct gggcctgggc cagcaggagg 2701 ccaagctcta cgggaagaag cgcaagtggg ggggcactgt ggttgctgag ccccagcaga 2761 aagagaagaa gggggtcgtc cccacccggc aggcactggc tgtgcccaca gactcggcgg 2821 tcaccgtgtc ctcctatgcc tacacgaagg tcactggccc ctacagccgc tggatctacc 2881 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2941 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 3001 tta