Construct: ORF ccsbBroad304_14486

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010881.1_s304c1, BRDN0000407548
Derived from:
ccsbBroadEn_14486
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ERCC6L2 (375748)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14486
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XM_011518650.3 54.6% 54.5% 1_1143del;1150T>C;1425G>T
2 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XM_017014707.2 50.4% 50.4% 1_1350del;1357T>C;1632G>T
3 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XM_011518648.3 45.6% 45.6% 1_1638del;1645T>C;1920G>T
4 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XM_011518644.3 36.3% 36.3% 1_2409del;2416T>C;2691G>T
5 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XM_011518641.3 30.4% 30.3% 1_3150del;3157T>C;3432G>T
6 human 375748 ERCC6L2 ERCC excision repair 6 like 2 NM_020207.5 29.4% 29.4% 1_3303del;3310T>C;3585G>T
7 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XR_001746290.2 27.3% (many diffs)
8 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XR_001746291.2 20.9% (many diffs)
9 human 375748 ERCC6L2 ERCC excision repair 6 like 2 XR_929789.2 17.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1446
ORF length:
1380
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa acgttcaaat gagaaagttg ttaatcaaga gcagtcgtat gaatcaatgg
121ataaattttt agatggcgtt caggaagtgg cttatattca ctcaaaccag aatgtaattg
181gatcgagcaa agctgaaaat cacatgagcc gatgggcagc acatgacgta tttgagttga
241agcagttttc tcagctgcct gctaacatag ctgtttgcag ttctaagaca tataaagaaa
301aagtggatgc agatacattg ccacacacaa agaaaggcca gcaacctagt gaaggcagca
361tttcacttcc tctttacatt tcaaatcctg taaaccagaa gaagaaaaaa gtctaccata
421caaaccagac caccttcata attggagaaa caccaaaagg aatccgcaga aaacaatttg
481aagaaatggc ctcttatttt aactcgtctt ctgtaaacga atttgctaaa catataacca
541atgccacatc agaagaacga cagaaaatgc taagagactt ttatgcttct caatatccag
601aggtaaaaga attttttgtg gattctgtgt cacaattcaa caattcttcc tttgagaaag
661gagagcagcg cacccggaag aaatctgata aaagagaatc tcttataaaa ccaaggctgt
721cagattctga aaccttgtca tttaaagatt ctaccaacaa aatttctcaa gtttgcagcc
781taaaaacata taaaagaaaa tcagttaagt ttcagaatca tatttcctat agagaagagg
841tgttttttaa tgatgcagaa actaagaaat cacctgttag ttctactcaa gagattgaca
901gtgggaaaaa cagccaggca tccgaagata ctgtgacatc ccgttctctg aacagtgagt
961ctgaaacacg tgagagaagg ttagaaaata ccatgaaaga ccaacaggac ctcacaagaa
1021cgggcatttc aagaaaagaa ccccttctca aattggaaaa caaaaagata gaaaatccag
1081tgctggaaaa tacttctgtg ataagcttac ttggtgatac ctctattctt gatgaccttt
1141ttaaaagtca tgggaacagt cccacacaac tgccaaagaa agttctttca gggcccatgg
1201aaaaagcaaa acagagacca aaagatttct gggacatctt gaatgagcag aatgatgaga
1261gtcttagtaa actcacagac ttggcagtaa tagagactct gtgtgaaaaa gcacctctag
1321cagcaccctt taaaaggaga gaagagccag caacttctct ttggaaatca aatgagaaat
1381ttttatggaa gaaatttagc ccaagtgata cagatgaaaa cgcaaccaat acacagagta
1441ccacataccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
1501gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
1561acctctggat ta

Download FASTA (ORF) (Full)