Construct: ORF ccsbBroad304_14494

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017115.1_s304c1, BRDN0000462345
Derived from:
ccsbBroadEn_14494
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GTF2IRD2B (389524)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14494
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 389524 GTF2IRD2B GTF2I repeat domain contain... NM_001003795.3 99.9% 99.5% 2562C>T;2837_2838insC
2 human 84163 GTF2IRD2 GTF2I repeat domain contain... NM_173537.4 99.5% 98.8% (many diffs)
3 human 389524 GTF2IRD2B GTF2I repeat domain contain... NM_001368302.1 85.3% 85% 1_486del;3048C>T;3323_3324insC
4 human 84163 GTF2IRD2 GTF2I repeat domain contain... NM_001368300.1 85% 84.4% (many diffs)
5 human 84163 GTF2IRD2 GTF2I repeat domain contain... NM_001281447.1 10.4% 8.8% (many diffs)
6 human 389524 GTF2IRD2B GTF2I repeat domain contain... NM_001368301.1 10.4% 8.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2919
ORF length:
2850
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcccaggta gcagtgtcca ccctgcctgt tgaagaagag tcctcctcag
121agaccaggat ggtggtgaca ttcctcgtgt ctgccctcga atccatgtgt aaagaactgg
181ccaagtccaa ggcagaagtg gcctgcatcg cagtgtacga aacagacgtg tttgtcgtcg
241gaaccgagag aggatgcgct tttgttaatg ccaggacgga ttttcagaaa gattttgcaa
301aatactgcgt tgcagaggga ctgtgtgagg tgaaacctcc ctgccctgtg aacgggatgc
361aggtccactc gggcgaaacg gaaatactca ggaaggcagt ggaggactat ttctgctttt
421gttatggtaa agccttaggg acaacagtga tggtgcctgt tccctatgag aagatgctgc
481gagaccagtc ggctgtggta gtgcaggggc ttccggaagg cgttgccttt caacaccctg
541agaattacga ccttgcaacc ctgaaatgga ttttggagaa caaagcaggg atttcattca
601tcataaatag acccttccta ggaccagaga gtcagctggg tggccctggg atggtaacag
661atgcggagag atccatagta tcaccaagtg aaagctgcgg ccccatcaat gtgaaaactg
721aacccatgga agattctggc atttcactga aagcagaagc tgtctcagtc aagaaagaat
781cagaagatcc taattactat caatataata tgcaaggaag ccacccttct tccacaagca
841atgaagtaat agaaatggaa ttaccaatgg aagattccac tccgctggtc ccttcagaag
901aaccaaatga ggaccctgaa gccgaggtga aaatcgaagg aaacacaaat tcatccagtg
961ttacaaattc tgcagcaggt gttgaagatc ttaacatcgt tcaagtgact gttccagata
1021atgagaagga aagattatca agcattgaaa agattaaaca gctaagagaa caagttaatg
1081acctctttag ccgaaaattt ggtgaagcaa ttggcgtgga tttccctgtg aaagttccct
1141acaggaagat cacattcaac cctggctgtg tggtgattga tggcatgccc ccgggggtgg
1201tattcaaggc ccccggctat ctggaaatca gttccatgag gaggatcttg gaggcagctg
1261agtttatcaa attcacagtc atcaggccgc ttccagggct tgagctcagt aatgtgggaa
1321aacgcaagat agaccaggag ggccgtgtgt ttcaagaaaa gtgggagaga gcgtatttct
1381tcgtggaagt acagaatatt ccaacatgtc tcatatgcaa acaaagcatg tctgtgtcca
1441aagaatataa cctaagacgc cactatcaaa ccaatcacag caagcattat gaccagtata
1501cggaaagaat gcgtgacgag aagcttcacg agctgaaaaa agggctcagg aagtatctct
1561taggctcgtc agacaccgag tgtcccgagc aaaaacaagt gtttgcaaac ccaagtccaa
1621cccagaaatc ccccgtgcag cctgtagagg acctagctgg gaacttatgg gagaagttac
1681gtgaaaaaat caggtctttt gtggcatatt ctatcgcaat cgatgagatc acggatataa
1741ataataccac ccagttggcc atattcatcc gtggtgtcga tgagaatttc gatgtgtccg
1801aagaacttct ggacacggtg cccatgacgg gtacaaaatc tggcaacgag atctttttgc
1861gtgttgagaa gagcctgaaa aagttctgta tcaactggtc gagattagta agcgtggcct
1921ccactggcac cccagcgatg gtggatgcca ataacgggct tgtcacaaaa ctgaagtcca
1981gggtggcgac gttctgcaag ggtgcggaac tgaagtccat ctgttgtata attcatccgg
2041aatcactctg tgctcagaag ttgaagatgg accacgtcat ggacgtggta gtgaagtccg
2101tgaactggat atgctcccgg ggactgaacc acagcgagtt cacaaccttg ctctatgagc
2161tggacagcca gtatggtagc ctcctgtact acacggagat taagtggctc agtcgcgggc
2221tcgtgctaaa gagatttttc gaatccttgg aagaaatcga ctccttcatg tcatccagag
2281ggaaacccct gcctcaactg agctccatag attggatccg agacctggcc ttcttggttg
2341acatgacgat gcatctgaac gctttgaaca tctctctcca aggacactcc caaatcgtca
2401cgcagatgta tgacctgatc cgggcgttcc tagcaaaact gtgcctctgg gagactcatt
2461tgacgaggaa taatctggcc cactttccca ccctgaaatt ggtttccaga aatgaaagcg
2521atggcctgaa ctacattccc aaaatcgcgg aactcaagac cgaattccag aaaaggctgt
2581ctgatttcaa actctacgaa agcgaactga ctctgttcag ctccccgttt tccacgaaga
2641tcgacagtgt gcacgaggag ctccagatgg aggttatcga cctgcaatgc aacacggtcc
2701tgaagacgaa atacgacaag gtgggaatac cagaattcta caagtacctc tggggtagct
2761acccgaaata caagcaccat tgcgcaaaga ttctttccat gttcgggagc acctacatct
2821gcgaacagct gttctccatt atgaaactga gcaaaacaaa atactgctcc cagttaaagg
2881attcccagtg ggattctgta ctccaccatc gcaacgttgc caactttctt gtacaaagtg
2941gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc
3001gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta

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