Construct: ORF ccsbBroad304_14534
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007909.1_s304c1, BRDN0000400467
- Derived from:
- ccsbBroadEn_14534
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- AP2A1 (160)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14534
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 160 | AP2A1 | adaptor related protein com... | NM_130787.3 | 93.7% | 58.6% | (many diffs) |
| 2 | human | 160 | AP2A1 | adaptor related protein com... | NM_014203.3 | 91.7% | 57.2% | (many diffs) |
| 3 | human | 160 | AP2A1 | adaptor related protein com... | XM_011526557.3 | 91.2% | 55.8% | (many diffs) |
| 4 | human | 160 | AP2A1 | adaptor related protein com... | XM_011526556.2 | 89.3% | 54.6% | (many diffs) |
| 5 | human | 161 | AP2A2 | adaptor related protein com... | XM_011519930.3 | 54.9% | 77.4% | (many diffs) |
| 6 | mouse | 11771 | Ap2a1 | adaptor-related protein com... | NM_001077264.1 | 84.7% | 58.4% | (many diffs) |
| 7 | mouse | 11771 | Ap2a1 | adaptor-related protein com... | NM_007458.2 | 82.8% | 57.1% | (many diffs) |
| 8 | mouse | 11771 | Ap2a1 | adaptor-related protein com... | XM_006540569.3 | 76.1% | 49.4% | (many diffs) |
| 9 | mouse | 11771 | Ap2a1 | adaptor-related protein com... | XM_006540570.1 | 51.7% | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1782
- ORF length:
- 1716
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggccgtgtcc aagggcgatg ggatgcgggg gctcgcggtg ttcatctccg 121 acatccggaa ctgtaagagc aaagaggcgg aaattaagag aatcaacaag gaactggcca 181 acatccgctc caagttcaaa ggagacaaag ccttggatgg ctacagtaag aaaaaatatg 241 tgtgtaaact gcttttcatc ttcctgcttg gccatgacat tgactttggg cacatggagg 301 ctgtgaatct gttgagttcc aataaataca cagagaagca aataggttac ctgttcattt 361 ctgtgctggt gaactcgaac tcggagctga tccgcctcat caacaacgcc atcaagaatg 421 acctggccag ccgcaacccc accttcatgt gcctggccct gcactgcatc gccaacgtgg 481 gcagccggga gatgggcgag gcctttgccg ctgacatccc ccgcatcctg gtggccgggg 541 acagcatgga cagtgtcaag cagagtgcgg ccctgtgcct ccttcgactg tacaaggcct 601 cgcctgacct ggtgcccatg ggcgagtgga cggcgcgtgt ggtacacctg ctcaatgacc 661 agcacatggg tgtggtcacg gccgccgtca gcctcatcac ctgtctctgc aagaagaacc 721 cagatgactt caagacgtgc gtctctctgg ctgtgtcgcg cctgagccgg atcgtctcct 781 ctgcctccac cgacctccag gactacacct actacttcgt cccagcaccc tggctctcgg 841 tgaagctcct gcggctgctg cagtgctacc cgcttccaga ggatgcggct gtgaaggggc 901 ggctggtgga atgtctggag actgtgctca acaaggccca ggagcccccc aaatccaaga 961 aggtgcagca ttccaacgcc aagaacgcca tcctcttcga gaccatcagc ctcatcatcc 1021 actatgacag tgagcccaac ctcctggttc gggcctgcaa ccagctgggc cagttcctgc 1081 agcaccggga gaccaacctg cgctacctgg ccctggagag catgtgcacg ctggccagct 1141 ccgagttctc ccatgaagcc gtcaagacgc acattgacac cgtcatcaat gccctcaaga 1201 cggagcggga cgtcagcgtg cggcagcggg cggctgacct cctctacgcc atgtgtgacc 1261 ggagcaatgc caagcagatc gtgtcggaga tgctgcggta cctggagacg gcagactacg 1321 ccatccgcga ggagatcgtc ctgaaggtgg ccatcctggc cgagaagtac gccgtggact 1381 acagctggta cgtggacacc atcctcaacc tcatccgcat tgcgggcgac tacgtgagtg 1441 aggaggtgtg gtaccgtgtg ctacagatcg tcaccaaccg tgatgacgtc cagggctatg 1501 ccgccaagac cgtctttgag gcgctccagg cccctgcctg tcacgagaac atggtgaagg 1561 ttggcggcta catccttggg gagtttggga acctgattgc tggggacccc cgctccagcc 1621 ccccagtgca gttctccctg ctccactcca agttccatct gtgcagcgtg gccacgcggg 1681 cgctgctgct gtccacctac atcaagttca tcaacctctt ccccgagacc aaggccacca 1741 tccagnggng ntctgcgggc cggctcccag ctgcgcaatg ctgacgtgga gctgcagcag 1801 cgagccgtgg agtacctcac cctcagctca gtggccagca ccgacgtcct ggccacggtg 1861 ctggaggaga tgccgccctt ccccgagcgc gagtcgtcca tcctggccaa gctgaaacgc 1921 aagaaggggc caggggccgg cagcgccctg gacgatggcc ggagggaccc cagcagcaac 1981 gacatcaacg ggggcatgga gcccaccccc agcactgtgt cgacgccctc gccctccgcc 2041 gacctcctgg ggctgcgggc agcccctccc ccggcagcac ccccggcttc tgcaggagca 2101 gggaaccttc tggtggacgt cttcgatggc ccggccgccc agcccagcct ggggcccacc 2161 cccgaggagg ccttcctcag cccaggtcct gaggacatcg gccctcccat tccggaagcc 2221 gatgagttgc tgaataagtt tgtgtgtaag aacaacgggg tcctgttcga gaaccagctg 2281 ctgcagatcg gagtcaagtc agagttccga cagaacctgg gccgcatgta tctcttctat 2341 ggcaacaaga cctcggtgca gttccagaat ttctcaccca ctgtggttca cccgggagac 2401 ctccagactc agctggctgt gcagaccaag cgcgtggcgg cgcaggtgga cggcggcgcg 2461 caggtgcagc aggtgctcaa tatcgagtgc ctgcgggact tcctgacgcc cccgctgctg 2521 tccgtgcgct tccggtacgg tggcgccccc caggccctca ccctgaagct cccagtgacc 2581 atcaacaagt tcttccagcc caccgagatg gcggcccagg atttcttcca gcgctggaag 2641 cagctgagcc tccctcaaca ggaggcgcag aaaatcttca aagccaacca ccccatggac 2701 gcagaagtta ctaaggccaa gcttctgggg tttggctctg ctctcctgga caatgtggac 2761 cccaaccctg gtgacagaga agacaccagg gtttggggga tgcctgggac tttcctccgg 2821 ccttttgtat ttttattttt gttcatctgc tgctgtttac attctggggg gttaggggga 2881 gtccccctcc ctccctttcc cccccaagca cagaggggag aggggccagg gaagtggatg 2941 tctcctcccc tcccacccca ccctgttgta gcccctccta ccccgcccnc atccaggggc 3001 tgtgtattat tgtgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct 3061 ctcctcggtc tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg 3121 acaatcaacc tctggatta