Construct: ORF ccsbBroad304_14544

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010977.2_s304c1, BRDN0000459641
Derived from:
ccsbBroadEn_14544
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ARAF (369)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14544
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 369 ARAF A-Raf proto-oncogene, serin... NM_001654.5 100% 100%
2 human 369 ARAF A-Raf proto-oncogene, serin... NM_001256196.1 99.5% 99.5% 459_467delGCCCTCAAG
3 human 369 ARAF A-Raf proto-oncogene, serin... NM_001256197.1 30.6% 30.5% 558_558delGins1261
4 mouse 11836 Araf Araf proto-oncogene, serine... NM_009703.2 89.9% 95.2% (many diffs)
5 mouse 11836 Araf Araf proto-oncogene, serine... XM_006527556.3 89.9% 95.2% (many diffs)
6 mouse 11836 Araf Araf proto-oncogene, serine... XM_006527557.3 89.9% 95.2% (many diffs)
7 mouse 11836 Araf Araf proto-oncogene, serine... XM_011247429.2 84.8% 89.7% (many diffs)
8 mouse 11836 Araf Araf proto-oncogene, serine... XM_011247430.2 67.1% 69.1% (many diffs)
9 mouse 11836 Araf Araf proto-oncogene, serine... NM_001159645.1 27.7% 29% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1884
ORF length:
1818
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gccaccacgg ggcccccctg ccaatggggc cgagccatcc cgggcagtgg
121gcaccgtcaa agtatacctg cccaacaagc aacgcacggt ggtgactgtc cgggatggca
181tgagtgtcta cgactctcta gacaaggccc tgaaggtgcg gggtctaaat caggactgct
241gtgtggtcta ccgactcatc aagggacgaa agacggtcac tgcctgggac acagccattg
301ctcccctgga tggcgaggag ctcattgtcg aggtccttga agatgtcccg ctgaccatgc
361acaattttgt acggaagacc ttcttcagcc tggcgttctg tgacttctgc cttaagtttc
421tgttccatgg cttccgttgc caaacctgtg gctacaagtt ccaccagcat tgttcctcca
481aggtccccac agtctgtgtt gacatgagta ccaaccgcca acagttctac cacagtgtcc
541aggatttgtc cggaggctcc agacagcatg aggctccctc gaaccgcccc ctgaatgagt
601tgctaacccc ccagggtccc agcccccgca cccagcactg tgacccggag cacttcccct
661tccctgcccc agccaatgcc cccctacagc gcatccgctc cacgtccact cccaacgtcc
721atatggtcag caccacggcc cccatggact ccaacctcat ccagctcact ggccagagtt
781tcagcactga tgctgccggt agtagaggag gtagtgatgg aaccccccgg gggagcccca
841gcccagccag cgtgtcctcg gggaggaagt ccccacattc caagtcacca gcagagcagc
901gcgagcggaa gtccttggcc gatgacaaga agaaagtgaa gaacctgggg taccgggact
961caggctatta ctgggaggta ccacccagtg aggtgcagct gctgaagagg atcgggacgg
1021gctcgtttgg caccgtgttt cgagggcggt ggcatggcga tgtggccgtg aaggtgctca
1081aggtgtccca gcccacagct gagcaggccc aggctttcaa gaatgagatg caggtgctca
1141ggaagacgcg acatgtcaac atcttgctgt ttatgggctt catgacccgg ccgggatttg
1201ccatcatcac acagtggtgt gagggctcca gcctctacca tcacctgcat gtggccgaca
1261cacgcTTCGA CATGGTCCAG CTCATCGACG TGGCCCGGCA GACTGCCCAG GGCATGGACT
1321ACCTCCATGC CAAGAACATC ATCCACCGAG ATCTCAAGTC TAACAACATC TTCCTACATG
1381AGGGGCTCAC GGTGAAGATC GGTGACTTTG GCTTGGCCAC AGTGAAGACT CGATGGAGCG
1441GGGCCCAGCC CTTGGAGCAG CCCTCAGGAT CTGTGCTGTG GATGGCAGCT GAGGTGATCC
1501GTATGCAGGA CCCGAACCCC TACAGCTTCC AGTCAGACGT CTATGCCTAC GGGGTTGTGC
1561TCTACGAGCT TATGACTGGC TCACTGCCTT ACAGCCACAT TGGCTGCCGT GACCAGATTA
1621TCTTTATGGT GGGCCGTGGC TATCTGTCCC CGGACCTCAG CAAAATCTCC AGCAACTGCC
1681CCAAGGCCAT GCGGCGCCTG CTGTCTGACT GCCTCAAGTT CCAGCGGGAG GAGCGGCCCC
1741TCTTCCCCCA GATCCTGGCC ACAATTGAGC TGCTGCAACG GTCACTCCCC AAGATTGAGC
1801GGAGTGCCTC GGAACCCTCC TTGCACCGCA CCCAGGCCGA TGAGTTGCCT GCCTGCCTAC
1861TCAGCGCAGC CCGCCTTGTG CCTTACCCAA CTTTCTTGTA Caaagtggtt ggtaagccta
1921tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
1981gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)