Transcript: Mouse NM_001159645.1

Mus musculus Araf proto-oncogene, serine/threonine kinase (Araf), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Araf (11836)
Length:
1571
CDS:
138..698

Additional Resources:

NCBI RefSeq record:
NM_001159645.1
NBCI Gene record:
Araf (11836)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001159645.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000054914 CTGCGTTGACATGAGTACCAA pLKO.1 566 CDS 100% 3.000 4.200 N Araf n/a
2 TRCN0000287252 CTGCGTTGACATGAGTACCAA pLKO_005 566 CDS 100% 3.000 4.200 N Araf n/a
3 TRCN0000054916 CTGTCAAAGTGTACCTGCCTA pLKO.1 196 CDS 100% 2.640 2.112 N Araf n/a
4 TRCN0000022610 CAGGCTCATCAAAGGAAGAAA pLKO.1 323 CDS 100% 5.625 3.938 N Araf n/a
5 TRCN0000307404 CAGGCTCATCAAAGGAAGAAA pLKO_005 323 CDS 100% 5.625 3.938 N Araf n/a
6 TRCN0000022612 GCATGAGTGTCTATGACTCTT pLKO.1 250 CDS 100% 4.950 3.465 N Araf n/a
7 TRCN0000294818 GCATGAGTGTCTATGACTCTT pLKO_005 250 CDS 100% 4.950 3.465 N Araf n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001159645.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15358 pDONR223 0% 27.7% 29% None (many diffs) n/a
2 ccsbBroad304_15358 pLX_304 0% 27.7% 29% V5 (many diffs) n/a
3 TRCN0000466363 CCTGGGTTTACAGATGTGGCTTAC pLX_317 12.9% 27.7% 29% V5 (many diffs) n/a
4 ccsbBroadEn_14544 pDONR223 0% 27.7% 29% None (many diffs) n/a
5 ccsbBroad304_14544 pLX_304 34.2% 27.7% 29% V5 (many diffs) n/a
6 TRCN0000466438 TTAATCGCAATTCTTCAACCCGCT pLX_317 18.8% 27.7% 29% V5 (many diffs) n/a
7 TRCN0000487716 TACTCTTTCGACAGCCAACCCGGG pLX_317 13% 27.7% 29% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000481643 GATAACGTGACCGCTAGGGGTCCA pLX_317 17.9% 27.6% 28.8% V5 (not translated due to frame shift) (many diffs) n/a
9 ccsbBroadEn_13813 pDONR223 100% 27.6% 28.8% None (many diffs) n/a
10 ccsbBroad304_13813 pLX_304 0% 27.6% 28.8% V5 (many diffs) n/a
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