Construct: ORF ccsbBroad304_14625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019043.1_s304c1, BRDN0000459842
- Derived from:
- ccsbBroadEn_14625
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA1 (2041)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14625
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2041 | EPHA1 | EPH receptor A1 | NM_005232.5 | 99.9% | 99.8% | 479T>C |
| 2 | human | 2041 | EPHA1 | EPH receptor A1 | XR_428169.4 | 93.3% | 1_60del;539T>C;2989_3134del | |
| 3 | human | 2041 | EPHA1 | EPH receptor A1 | XM_006715880.3 | 88.1% | 88.1% | 479T>C;988_989ins345 |
| 4 | mouse | 13835 | Epha1 | Eph receptor A1 | NM_023580.4 | 85.7% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2997
- ORF length:
- 2928
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcggcgc tggcccctgg ggctagggct ggtgctgctg ctctgcgccc 121 cgctgccccc gggggcgcgc gccaaggaag ttactctgat ggacacaagc aaggcacagg 181 gagagctggg ctggctgctg gatcccccaa aagatgggtg gagtgaacag caacagatac 241 tgaatgggac acccctgtac atgtaccagg actgcccaat gcaaggacgc agagacactg 301 accactggct tcgctccaat tggatctacc gcggggagga ggcttcccgc gtccacgtgg 361 agctgcagtt caccgtgcgg gactgcaaga gtttccctgg gggagccggg cctctgggct 421 gcaaggagac cttcaacctt ctgtacatgg agagtgacca ggatgtgggc attcagctcc 481 gacggccctt gttccagaag gtaaccacgg tggctgcaga ccagagcttc accattcgag 541 accttgcgtc tggctccgtg aagctgaatg tggagcgctg ctctctgggc cgcctgaccc 601 gccgtggcct ctacctcgct ttccacaacc cgggtgcctg tgtggccctg gtgtctgtcc 661 gggtcttcta ccagcgctgt cctgagaccc tgaatggctt ggcccaattc ccagacactc 721 tgcctggccc cgctgggttg gtggaagtgg cggggacctg cttgccccac gcgcgggcca 781 gccccaggcc ctcaggtgca ccccgcatgc actgcagccc tgatggcgag tggctggtgc 841 ctgtaggacg gtgccactgt gagcctggct atgaggaagg tggcagtggc gaagcatgtg 901 ttgcctgccc tagcggctcc taccggatgg acatggacac accccattgt ctcacgtgcc 961 cccagcagag cactgctgag tctgaggggg ccaccatctg tacctgtgag agcggccatt 1021 acagagctcc cggggagggc ccccaggtgg catgcacagg tcccccctcg gccccccgaa 1081 acctgagctt ctctgcctca gggactcagc tctccctgcg ttgggaaccc ccagcagata 1141 cggggggacg ccaggatgtc agatacagtg tgaggtgttc ccagtgtcag ggcacagcac 1201 aggacggggg gccctgccag ccctgtgggg tgggcgtgca cttctcgccg ggggcccggg 1261 ggctcaccac acctgcagtg catgtcaatg gccttgaacc ttatgccaac tacaccttta 1321 atgtggaagc ccaaaatgga gtgtcagggc tgggcagctc tggccatgcc agcacctcag 1381 tcagcatcag catggggcat gcagagtcac tgtcaggcct gtctctgaga ctggtgaaga 1441 aagaaccgag gcaactagag ctgacctggg cggggtcccg gccccgaagc cctggggcga 1501 acctgaccta tgagctgcac gtgctgaacc aggatgaaga acggtaccag atggttctag 1561 aacccagggt cttgctgaca gagctgcagc ctgacaccac atacatcgtc agagtccgaa 1621 tgctgacccc actgggtcct ggccctttct cccctgatca tgagtttcgg accagcccac 1681 cagtgtccag gggcctgact ggaggagaga ttgtagccgt catctttggg ctgctgcttg 1741 gtgcagcctt gctgcttggg attctcgttt tccggtccag gagagcccag cggcagaggc 1801 agcagaggca gcgtgaccgc gccaccgatg tggatcgaga ggacaagctg tggctgaagc 1861 cttatgtgga cctccaggca tacgaggacc ctgcacaggg agccctggac tttacccggg 1921 agcttgatcc agcgtggctg atggtggaca ctgtcatagg agaaggagag tttggggaag 1981 tgtatcgagg gaccctgagg ctccccagcc aggactgcaa gactgtggcc attaagacct 2041 taaaagacac atccccaggt ggccagtggt ggaacttcct tcgagaggca actatcatgg 2101 gccagtttag ccacccgcat attctgcatc tggaaggcgt cgtcacaaag cgaaagccga 2161 tcatgatcat cacagaattt atggagaatg gagccctgga tgccttcctg agggagcggg 2221 aggaccagct ggtccctggg cagctagtgg ccatgctgca gggcatagca tctggcatga 2281 actacctcag taatcacaat tatgtccacc gggacctggc tgccagaaac atcttggtga 2341 atcaaaacct gtgctgcaag gtgtctgact ttggcctgac tcgcctcctg gatgactttg 2401 atggcacata cgaaacccag ggaggaaaga tccctatccg ttggacagcc cctgaagcca 2461 ttgcccatcg gatcttcacc acagccagcg atgtgtggag ctttgggatt gtgatgtggg 2521 aggtgctgag ctttggggac aagccttatg gggagatgag caatcaggag gttatgaaga 2581 gcattgagga tgggtaccgg ttgccccctc ctgtggactg ccctgcccct ctgtatgagc 2641 tcatgaagaa ctgctgggca tatgaccgtg cccgccggcc acacttccag aagcttcagg 2701 cacatctgga gcaactgctt gccaaccccc actccctgcg gaccattgcc aactttgacc 2761 ccaggatgac tcttcgcctg cccagcctga gtggctcaga tgggatccca tatcgaaccg 2821 tctctgagtg gctcgagtcc atacgcatga aacgctacat cctgcacttc cactcggctg 2881 ggctggacac catggagtgt gtgctggagc tgaccgctga ggacctgacg cagatgggaa 2941 tcacactgcc cgggcaccag aagcgcattc tttgcagtat tcagggattc aaggacttgc 3001 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 3061 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 3121 tta