Construct: ORF ccsbBroad304_14666
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012068.1_s304c1, BRDN0000407193
- Derived from:
- ccsbBroadEn_14666
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HK2 (3099)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14666
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3099 | HK2 | hexokinase 2 | NM_000189.5 | 99.4% | 99.2% | (many diffs) |
| 2 | human | 3099 | HK2 | hexokinase 2 | XM_005264280.2 | 97.2% | 97% | (many diffs) |
| 3 | human | 3099 | HK2 | hexokinase 2 | NM_001371525.1 | 96.4% | 96.1% | (many diffs) |
| 4 | human | 3099 | HK2 | hexokinase 2 | XM_017003945.2 | 96% | 95.7% | (many diffs) |
| 5 | human | 3099 | HK2 | hexokinase 2 | XM_011532807.2 | 93.8% | 93.6% | (many diffs) |
| 6 | mouse | 15277 | Hk2 | hexokinase 2 | NM_013820.3 | 87% | 93.4% | (many diffs) |
| 7 | mouse | 15277 | Hk2 | hexokinase 2 | XM_017321401.1 | 76.8% | 82.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2817
- ORF length:
- 2751
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat tgcctcgcat ctgcttgcct acttcttcac ggagctcaac catgaccaag 121 tgcagaaggt tgaccagtat ctctaccaca tgcgcctctc tgatgagacc ctcttggaga 181 tctctaagcg gttccgcaag gagatggaga aagggcttgg agccaccact caccctactg 241 cagcagtgaa gatgctgccc acctttgtga ggtccactcc agatgggaca gaacacggag 301 agttcctggc tctggatctt ggagggacca acttccgtgt gctttgggtg aaagtaacgg 361 acaatgggct ccagaaggtg gagatggaga atcagatcta tgccatccct gaggacatca 421 tgcgaggcag tggcacccag ctgtttgacc acattgccga atgcctggct aacttcatgg 481 ataagctaca aatcaaagac aagaagctcc cactgggttt taccttctcg ttcccctgcc 541 accagactaa actagacgag agtttcctgg tctcatggac caagggattc aagtccagtg 601 gagtggaagg cagagacgtt gtggctctga tccggaaggc catccagagg agaggggact 661 ttgatatcga cattgtggct gtggtgaatg acacagttgg gaccatgatg acctgtggtt 721 atgatgacca caactgtgag attggtctca ttgtgggcac gggcagcaac gcctgctaca 781 tggaagagat gcgccacatc gacatggtgg aaggcgatga ggggcggatg tgtgtgaaca 841 tggaatgggg ggccttcggg gacaatggct cgctcaacga cattcgcact gagtttgacc 901 aggagattga catgggctca ctgaacccgg gaaagcaact gtttgagaag atgatcagtg 961 ggatgtacat gggggagctg gtgaggctta tcctggtgaa gatggccaag gaggagctgc 1021 tctttggggg gaagctcagc ccagagcttc tcaacaccgg tcgctttgag accaaagaca 1081 tctcagacat tgaaggggag aaggatggca tccggaaggc ccgtgaggtc ctgatgcggt 1141 tgggcctgga cccgactcag gaggactgcg tggccactca ccggatctgc cagatcgtgt 1201 ccacacgctc cgccagcctg tgcgcagcca ccctggccgc cgtgctgcag cgcatcaagg 1261 agaacaaagg cgaggagcgg ctgcgctcta ctattggggt cgacggttcc gtctacaaga 1321 aacaccccca ttttgccaag cgtctacata agaccgtgcg gcggctggtg cccggctgcg 1381 atgtccgctt cctccgctcc gaggatggca gtggcaaagg tgcagccatg gtgacagcag 1441 tggcttaccg gctggccgat caacaccgtg cccgccagaa gacattagag catctgcagc 1501 tgagccatga ccagctgctg gaggtcaaga ggaggatgaa ggtagaaatg gagcgaggtc 1561 tgagcaagga gactcatgcc agtgcccccg tcaagatgct gcccacctac gtgtgtgcta 1621 ccccggacgg cacagagaaa ggggacttct tggccttgga ccttggagga acaaatttcc 1681 gggtcctgct ggtccgtgtt cggaatggga agtggggtgg agtggagatg cacaacaaga 1741 tctacgccat cccgcaggag gtcatgcacg gcaccgggga cgagctcttt gaccacattg 1801 tccagtgcat cgcggacttc ctcgagtaca tgggcatgaa gggcgtgtcc ctgcctctgg 1861 gttttacctt ctccttcccc tgccagcaga acagcctgga cgagagcatc ctcctcaagt 1921 ggacaaaagg cttcaaggca tctggctgcg agggcgagga cgtggtgacc ctgctgaagg 1981 aagcgatcca ccggcgagag gagtttgacc tggatgtggt tgctgtggtg aacgacacag 2041 tcggaactat gatgacctgt ggctttgaag accctcactg tgaagttggc ctcattgttg 2101 gcacgggcag caatgcctgc tacatggaag agatgcgcca catcgacatg gtggaaggcg 2161 atgaggggcg gatgtgtgtg aacatggaat ggggggcctt cggggacaat ggatgcctag 2221 atgacttccg cacagaattt gatgtggctg tggatgagct ttcactcaac cccggcaagc 2281 agaggttcga gaaaatgatc agtggaatgt acctgggtga gattgtccgt aacattctca 2341 tcgatttcac caagcgtgga ctgctcttcc gaggccgcat ctcagagcgg ctcaagacaa 2401 ggggcatctt tgaaaccaag ttcttgtctc agattgagag tgactgcctg gccctgctgc 2461 aagtccgagc catcctgcaa cacttagggc ttgagagcac ctgtgacgac agcatcattg 2521 ttaaggaggt gtgcactgtg gtggcccggc gggcagccca gctctgtggc gcaggcatgg 2581 ccgctgtggt ggacaggata cgagaaaacc gtgggctgga cgctctcaaa gtgacagtgg 2641 gtgtggatgg gaccctctac aagctacatc ctcactttgc caaagtcatg catgagacag 2701 tgaaggacct ggctccgaaa tgtgatgtgt ctttcctgca gtcagaggat ggcagcggga 2761 agggggcggc gctcatcact gctgtggcct gccgcatccg tgaggctgga cagcgatacc 2821 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 2881 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 2941 tta