Construct: ORF ccsbBroad304_14688

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019004.1_s304c1, BRDN0000459805
Derived from:
ccsbBroadEn_14688
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LCK (3932)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14688
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3932 LCK LCK proto-oncogene, Src fam... NM_001042771.2 100% 100%
2 human 3932 LCK LCK proto-oncogene, Src fam... NM_005356.5 100% 100%
3 human 3932 LCK LCK proto-oncogene, Src fam... NM_001330468.2 89.9% 89.7% 631_632ins153
4 human 3932 LCK LCK proto-oncogene, Src fam... XM_024447046.1 89.7% 89.7% 186_359del
5 human 3932 LCK LCK proto-oncogene, Src fam... XM_024447047.1 89.7% 89.7% 186_359del
6 human 3932 LCK LCK proto-oncogene, Src fam... XM_011541453.3 80.7% 80.5% 186_359del;805_806ins153
7 mouse 16818 Lck lymphocyte protein tyrosine... NM_001162433.1 88.6% 96.8% (many diffs)
8 mouse 16818 Lck lymphocyte protein tyrosine... NM_010693.3 88.6% 96.8% (many diffs)
9 mouse 16818 Lck lymphocyte protein tyrosine... XM_006502818.3 88.6% 96.8% (many diffs)
10 mouse 16818 Lck lymphocyte protein tyrosine... XM_030253275.1 88.6% 96.8% (many diffs)
11 mouse 16818 Lck lymphocyte protein tyrosine... XM_030253276.1 88.6% 96.8% (many diffs)
12 mouse 16818 Lck lymphocyte protein tyrosine... NM_001162432.1 86.7% 94.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1596
ORF length:
1527
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gggctgtggc tgcagctcac acccggaaga tgactggatg gaaaacatcg
121atgtgtgtga gaactgccat tatcccatag tcccactgga tggcaagggc acgctgctca
181tccgaaatgg ctctgaggtg cgggacccac tggttaccta cgaaggctcc aatccgccgg
241cttccccact gcaagacaac ctggttatcg ctctgcacag ctatgagccc tctcacgacg
301gagatctggg ctttgagaag ggggaacagc tccgcatcct ggagcagagc ggcgagtggt
361ggaaggcgca gtccctgacc acgggccagg aaggcttcat ccccttcaat tttgtggcca
421aagcgaacag cctggagccc gaaccctggt tcttcaagaa cctgagccgc aaggacgcgg
481agcggcagct cctggcgccc gggaacactc acggctcctt cctcatccgg gagagcgaga
541gcaccgcggg atcgttttca ctgtcggtcc gggacttcga ccagaaccag ggagaggtgg
601tgaaacatta caagatccgt aatctggaca acggtggctt ctacatctcc cctcgaatca
661cttttcccgg cctgcatgaa ctggtccgcc attacaccaa tgcttcagat gggctgtgca
721cacggttgag ccgcccctgc cagacccaga agccccagaa gccgtggtgg gaggacgagt
781gggaggttcc cagggagacg ctgaagctgg tggagcggct gggggctgga cagttcgggg
841aggtgtggat ggggtactac aacgggcaca cgaaggtggc ggtgaagagc ctgaagcagg
901gcagcatgtc cccggacgcc ttcctggccg aggccaacct catgaagcag ctgcaacacc
961agcggctggt tcggctctac gctgtggtca cccaggagcc catctacatc atcactgaat
1021acatggagaa tgggagtcta gtggattttc tcaagacccc ttcaggcatc aagttgacca
1081tcaacaaact cctggacatg gcagcccaaa ttgcagaagg catggcattc attgaagagc
1141ggaattatat tcatcgtgac cttcgggctg ccaacattct ggtgtctgac acccTGAGCT
1201GCAAGATTGC AGACTTTGGC CTAGCACGCC TCATTGAGGA CAACGAGTAC ACAGCCAGGG
1261AGGGGGCCAA GTTTCCCATT AAGTGGACAG CGCCAGAAGC CATTAACTAC GGGACATTCA
1321CCATCAAGTC AGATGTGTGG TCTTTTGGGA TCCTGCTGAC GGAAATTGTC ACCCACGGCC
1381GCATCCCTTA CCCAGGGATG ACCAACCCGG AGGTGATTCA GAACCTGGAG CGAGGCTACC
1441GCATGGTGCG CCCTGACAAC TGTCCAGAGG AGCTGTACCA ACTCATGAGG CTGTGCTGGA
1501AGGAGCGCCC AGAGGACCGG CCCACCTTTG ACTACCTGCG CAGTGTGCTG GAGGACTTCT
1561TCACGGCCAC AGAGGGCCAG TACCAGCCTC AGCCTCCAAC TTTCTTGTAC aaagtggttg
1621gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1681accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)