Construct: ORF ccsbBroad304_14695

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018953.1_s304c1, BRDN0000459437
Derived from:
ccsbBroadEn_14695
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAP3K5 (4217)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14695
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4217 MAP3K5 mitogen-activated protein k... NM_005923.4 93.4% 3% (many diffs)
2 human 4217 MAP3K5 mitogen-activated protein k... XM_017010873.1 86.8% 2.8% (many diffs)
3 human 4217 MAP3K5 mitogen-activated protein k... XM_017010872.1 86.8% 2.8% (many diffs)
4 human 4217 MAP3K5 mitogen-activated protein k... XM_017010871.1 85.6% 2.7% (many diffs)
5 human 4217 MAP3K5 mitogen-activated protein k... XM_017010870.1 85.5% 2.7% (many diffs)
6 human 4217 MAP3K5 mitogen-activated protein k... XM_017010875.1 84.5% 2.8% (many diffs)
7 human 4217 MAP3K5 mitogen-activated protein k... XM_017010874.1 83.2% 2.8% (many diffs)
8 human 4217 MAP3K5 mitogen-activated protein k... XM_017010877.1 82.8% 1.1% (many diffs)
9 human 4217 MAP3K5 mitogen-activated protein k... XM_017010876.1 81.4% 1.1% (many diffs)
10 human 4217 MAP3K5 mitogen-activated protein k... XM_011535839.3 74% 3.2% (many diffs)
11 mouse 26408 Map3k5 mitogen-activated protein k... NM_008580.4 81.7% 2.5% (many diffs)
12 mouse 26408 Map3k5 mitogen-activated protein k... XM_006512739.3 61.2% 3.2% (many diffs)
13 mouse 26408 Map3k5 mitogen-activated protein k... XR_001779529.1 44.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
267
ORF length:
198
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gagcacggag gcggacgagg gcatcacttt ctctgtgcca cccttcgccc
121cctcgggctt ctgcaccatc cccgagggcg gcatctgcag gaggggagga gcggcggcgg
181tgtgtttttt tcactattca aacaggccat cgtcgcctcc ccggcaccgt tcactctcaa
241ccagtcggtc cgttcatact cttcagtaaa tgtgccataa gaatttaaat gaaatacatg
301caattcagga atcttcttgg gtagggaatt tagcttcacg tggataatat cttggtcttt
361ttcgtaaccc actagatgaa tgagtgctgg taattctttc tctgccccaa ctaattcttc
421agtactctcg agtcctctag tcacatcatt ctaaactgca cattcagtga catgtgagca
481ctccggcaat catgagatac actacaactg agcgtgctca tgcctactgg tggtggccga
541gagcgaggcc ctgcagagct tgcgggaggc gtgcgagaca gtgggcgcca ccctggaaac
601cctgcatttt gggaaactcg actttggaga aaccaccgtg ctggaccgct tttacaatgc
661agatattgcg gtggtggaga tgagcgatgc cttccggcag ccgtccttgt tttaccacct
721tggggtgaga gaaagtttca gcatggccaa caacatcatc ctctactgtg atactaactc
781ggactctctg cagtcactga aggaaatcat ttgccagaag aatactatgt gcactgggaa
841ctacaccttt gttccttaca tgataactcc acataacaaa gtctactgct gtgacagcag
901cttcatgaag gggttgacag agctcatgca accgaacttc gagctgcttc ttggacccat
961ctgcttacct cttgtggatc gttttattca acttttgaag gtggcacaag caagttctag
1021ccagtacttc cgggaatcta tactcaatga catcaggaaa gctcgtaatt tatacactgg
1081taaagaattg gcagctgagt tggcaagaat tcggcagcga gtagataata tcgaagtctt
1141gacagcagat attgtcataa atctgttact ttcctacaga gatatccagg actatgattc
1201tattgtgaag ctggtagaga ctttagaaaa actgccaacc tttgatttgg cctcccatca
1261ccatgtgaag tttcattatg catttgcact gaataggaga aatctccctg gtgacagagc
1321aaaagctctt gatattatga ttcccatggt gcaaagcgaa ggacaagttg cttcagatat
1381gtattgccta gttggtcgaa tctacaaaga tatgtttttg gactctaatt tcacggacac
1441tgaaagcaga gaccatggag cttcttggtt caaaaaggca tttgaatctg agccaacact
1501acagtcagga attaattatg cggtcctcct cctggcagct ggacaccagt ttgaatcttc
1561ctttgagctc cggaaagttg gggtgaagct aagtagtctt cttggtaaaa agggaaactt
1621ggaaaaactc cagagctact gggaagttgg attttttctg ggggccagcg tcctagccaa
1681tgaccacatg agagtcattc aagcatctga aaagcttttt aaactgaaga caccagcatg
1741gtacctcaag tctattgtag agacaatttt aatatataag cattttgtga aactgaccac
1801agaacagcct gtggccaagc aagaacttgt ggacttttgg atggatttcc tggtcgaggc
1861cacaaagaca gatgttactg tggttaggtt tccagtatta atattagaac caaccaaaat
1921ctatcaacct tcttatttgt ctatcaacaa tgaagttgag gaaaagacaa tctctatttg
1981gcacgtgctt cctgatgaca agaaaggtat acatgagtgg aattttagtg cctcttctgt
2041caggggagtg agtatttcta aatttgaaga aagatgctgc tttctttatg tgcttcacaa
2101ttctgatgat ttccaaatct atttctgtac agaacttcat tgtaaaaagt tttttgagat
2161ggtgaacacc attaccgaag agaaggggag aagcacagag gaaggagact gtgaaagtga
2221cttgctggag tatgactatg aatatgatga aaatggtgac agagtcgttt taggaaaagg
2281cacttatggg atagtctacg caggtcggga cttgagcaac caagtcagaa ttgctattaa
2341ggaaatccca gagagagaca gcagatactc tcagcccctg catgaagaaa tagcattgca
2401taaacacctg aagcacaaaa atattgtcca gtatctgggc tctttcagtg agaatggttt
2461cattaaaatc ttcatggagc aggtccctgg aggaagtctt tctgctctcc ttcgttccaa
2521atggggtcca ttaaaagaca atgagcaaac aattggcttt tatacaaagc aaatactgga
2581aggattaaaa tatctccatg acaatcagat agttcaccgg gacataaagg gtgacaatgt
2641gttgattaat acctacagtg gtgttctcaa gatctctgac ttcggaacat caaagaggct
2701tgctggcata aacccctgta ctgaaacttt tactggtacc ctccagtata tggcaccaga
2761aataatagat aaaggaccaa gaggctacgg aaaagcagca gacatctggt ctctgggctg
2821tacaatcatt gaaatggcca caggaaaacc cccattttat gaactgggag aaccacaagc
2881agctatgttc aaggtgggaa tgtttaaagt ccaccctgag atcccagagt ccatgtctgc
2941agaggccaag gcattcatac tgaaatgttt tgaaccagat cctgacaaga gagcctgtgc
3001taacgacttg cttgttgatg agtttttaaa agtttcaagc aaaaagaaaa agacacaacc
3061taagctttca gctctttcag ctggatcaaa tgaatatctc aggagtatat ccttgccggt
3121acctgtgctg gtggaggaca ccagcagcag cagtgagtac ggctcagttt cacccgacac
3181ggagttgaaa gtggacccct tctctttcaa aacaagagcc aagtcctgcg gagaaagaga
3241tgtcaaggga attcggacac tctttttggg cattccagat gagaattttg aagatcacag
3301tgctcctcct tcccctgaag aaaaagattc tggattcttc atgctgagga aggacagtga
3361gaggcgagct acccttcaca ggatcctgac ggaagaccaa gacaaaattg tgagaaacct
3421aatggaatct ttagctcagg gggctgaaga accgaaacta aaatgggaac acatcacaac
3481cctcattgca agcctcagag aatttgtgag atccactgac cgaaaaatca tagccaccac
3541actgtcaaag ctgaaactgg agctggactt cgacagccat ggcattagcc aagtccaggt
3601ggtactcttt ggttttcaag atgctgtcaa taaagttctt cggaatcata acatcaagcc
3661gcactggatg tttgccttag acagtatcat tcggaaggcg gtacagacag ccattaccat
3721cctggttcca gaactaaggc cacatttcag ccttgcatct gagagtgata ctgctgatca
3781agaagacttg gatgtagaag atgaccatga ggaacagcct tcaaatcaaa ctgtccgaag
3841acctcaggct gtcattgaag atgctgtggc tacctcaggc gtgagcacgc tcagttcTAC
3901TGTGTCTCAT GATTCCCAGA GTGCTCACCG GTCACTGAAT GTACAGCTTG GAAGGATGAA
3961AATAGAAACC AATAGATTAC TGGAAGAATT GGTTCGGAAA GAGAAAGAAT TACAAGCACT
4021CCTTCATCGA GCTATTGAAG AAAAAGACCA AGAAATTAAA CACCTGAAGC TTAAGTCCCA
4081ACCCATAGAA ATTCCTGAAT TGCCTGTATT TCATCTAAAT TCTTCTGGCA CAAATACTGA
4141AGATTCTGAA CTTACCGACT GGCTGAGAGT GAATGGAGCT GATGAAGACA CTATAAGCCG
4201GTTTTTGGCT GAAGATTATA CACTATTGGA TGTTCTCTAC TATGTTACAC GTGATGACTT
4261AAAATGCTTG AGACTAAGGG GAGGGATGCT GTGCACACTG TGGAAGGCTA TCATTGACTT
4321TCGAAACAAA CAGACTTTGC CAACTTTCTT GTACaaagtg gttggtaagc ctatccctaa
4381ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt ggcgcgttaa
4441gtcgacaatc aacctctgga tta

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