Construct: ORF ccsbBroad304_14708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006149.1_s304c1, BRDN0000400360
- Derived from:
- ccsbBroadEn_14708
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCBP1 (4686)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14708
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4686 | NCBP1 | nuclear cap binding protein... | NM_002486.5 | 99.9% | 100% | 1431T>C |
| 2 | human | 4686 | NCBP1 | nuclear cap binding protein... | NM_001351504.1 | 91.1% | 90.5% | (many diffs) |
| 3 | human | 4686 | NCBP1 | nuclear cap binding protein... | NM_001351505.1 | 75.9% | 65.6% | 0_1ins478;201_202ins92;861T>C |
| 4 | human | 4686 | NCBP1 | nuclear cap binding protein... | NM_001351506.1 | 75.9% | 65.6% | 0_1ins478;201_202ins92;861T>C |
| 5 | human | 4686 | NCBP1 | nuclear cap binding protein... | NM_001351507.1 | 53.1% | 53.1% | 0_1ins1110;321T>C |
| 6 | mouse | 433702 | Ncbp1 | nuclear cap binding protein... | NM_001033201.3 | 89.4% | 97.9% | (many diffs) |
| 7 | mouse | 433702 | Ncbp1 | nuclear cap binding protein... | XM_017320296.1 | 76.9% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2436
- ORF length:
- 2370
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gcggcggcgg cacagcgacg agaacgacgg tgggcagcct cacaaaagga 121 gaaagacctc tgatgcaaat gaaactgaag atcatttgga atctttaata tgtaaagtag 181 gagaaaagag tgcctgctct ttggagagca acctagaagg cttggctggt gttttggaag 241 ctgatcttcc taactacaag agcaagatct taaggcttct ttgtacagtt gcacgcctat 301 tacctgagaa gctgacaatt tatacaacat tagttggact actgaatgcc aggaattaca 361 attttggtgg agaatttgta gaagccatga ttcgtcaact taaagaatca ttgaaagcaa 421 acaattataa tgaagccgtg tatttggtcc gttttttatc tgatcttgtg aattgtcatg 481 tgattgccgc cccatcaatg gttgctatgt ttgaaaattt tgtaagcgta actcaggaag 541 aagatgtacc tcaggtgcga cgagattggt atgtgtatgc atttctgtca tctttgccct 601 gggttggaaa ggagttgtac gaaaagaaag atgcagagat ggaccgcatc tttgccaaca 661 ctgaaagcta tcttaaaaga cgccaaaaga ctcatgtacc catgttacag gtatggactg 721 ctgataaacc acatccacaa gaagagtatt tagattgcct gtgggcccag attcagaaat 781 tgaaaaagga tcgctggcag gaacggcaca tcctaagacc ttatcttgcc tttgacagca 841 tcctgtgtga agcactgcag cacaatctgc ctccttttac accacctcct cacactgaag 901 attcagtgta cccaatgcca agggtcatct tcagaatgtt tgattacaca gatgatcccg 961 agggtcctgt catgccaggg agtcattcag tggaaagatt tgtaatagaa gagaatcttc 1021 actgcatcat taagtcccac tggaaggaaa ggaagacttg tgctgcacag ttagtgagct 1081 atccagggaa gaacaagatc cccttgaact accacatagt tgaggtgatc tttgcagagc 1141 tgtttcaact tccagcaccc cctcacattg atgtgatgta cacaacactc ctcattgaac 1201 tgtgcaaact tcaacctggc tctctacccc aagttcttgc acaggcaact gaaatgctat 1261 acatgcgttt ggacacaatg aacactacct gtgtagacag gtttattaat tggttttctc 1321 atcatctaag taacttccag ttccgttgga gctgggaaga ttggtcagat tgtcttagtc 1381 aagatcctga aagtcccaaa ccgaagtttg taagagaagt tctagaaaaa tgtatgaggt 1441 tgtcttacca tcagcgtata ttagatattg ttcctcctac cttctcagct ctgtgccctg 1501 caaacccaac ctgcatttac aagtatggag atgaaagtag caattctctt cctggacatt 1561 ctgttgccct ctgtttagct gttgccttta aaagtaaggc aaccaatgat gaaatcttca 1621 gcattctgaa agatgtacca aatcctaacc aggatgatga cgacgatgaa ggattcagtt 1681 ttaacccatt gaaaatagaa gtctttgtac agactctgct acacttggca gccaaatcat 1741 tcagccactc cttcagtgct cttgcaaagt ttcatgaagt cttcaaaacc ctagctgaaa 1801 gtgatgaagg aaagttacat gtgctaagag ttatgtttga ggtctggagg aaccatccac 1861 agatgattgc tgtactagtg gataagatga ttcgtacaca aatagttgat tgtgctgccg 1921 tagcaaattg gatcttctct tcagaactat ctcgtgactt taccagattg tttgtttggg 1981 aaattttgca ctctacaatt cgtaagatga acaaacatgt cctgaagatc cagaaagagc 2041 tggaagaagc taaagagaaa cttgctaggc aacacaaacg gcgaagtgat gatgacgaca 2101 gaagcagtga caggaaagac ggggttcttg aggaacaaat agaacgactt caggaaaaag 2161 tggaatctgc tcagagtgaa caaaagaatc ttttcctcgt tatatttcag cggtttatca 2221 tgatcttgac cgagcaccta gtacgatgcg aaactgatgg gaccagtgta ttaacaccat 2281 ggtataagaa ctgtatagag aggctgcagc agatcttcct acagcatcac caaataatcc 2341 agcagtacat ggtgaccctg gagaaccttc tcttcactgc tgaattagac cctcatatct 2401 tggccgtgtt ccagcagttc tgtgccctgc aggcctaccc aactttcttg tacaaagtgg 2461 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2521 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta