Construct: ORF ccsbBroad304_14736
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019007.1_s304c1, BRDN0000459808
- Derived from:
- ccsbBroadEn_14736
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRB (5159)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14736
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_002609.4 | 100% | 100% | |
| 2 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355016.2 | 94.2% | 94.2% | 0_1ins192 |
| 3 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355017.2 | 83.5% | 81.8% | 0_1ins517;114_147del |
| 4 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_008809.2 | 83.1% | 85.4% | (many diffs) |
| 5 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_001146268.1 | 83% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3387
- ORF length:
- 3318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggcttccg ggtgcgatgc cagctctggc cctcaaaggc gagctgctgt 121 tgctgtctct cctgttactt ctggaaccac agatctctca gggcctggtc gtcacacccc 181 cggggccaga gcttgtcctc aatgtctcca gcaccttcgt tctgacctgc tcgggttcag 241 ctccggtggt gtgggaacgg atgtcccagg agcccccaca ggaaatggcc aaggcccagg 301 atggcacctt ctccagcgtg ctcacactga ccaacctcac tgggctagac acgggagaat 361 acttttgcac ccacaatgac tcccgtggac tggagaccga tgagcggaaa cggctctaca 421 tctttgtgcc agatcccacc gtgggcttcc tccctaatga tgccgaggaa ctattcatct 481 ttctcacgga aataactgag atcaccattc catgccgagt aacagaccca cagctggtgg 541 tgacactgca cgagaagaaa ggggacgttg cactgcctgt cccctatgat caccaacgtg 601 gcttttctgg tatctttgag gacagaagct acatctgcaa aaccaccatt ggggacaggg 661 aggtggattc tgatgcctac tatgtctaca gactccaggt gtcatccatc aacgtctctg 721 tgaacgcagt gcagactgtg gtccgccagg gtgagaacat caccctcatg tgcattgtga 781 tcgggaatga ggtggtcaac ttcgagtgga catacccccg caaagaaagt gggcggctgg 841 tggagccggt gactgacttc ctcttggata tgccttacca catccgctcc atcctgcaca 901 tccccagtgc cgagttagaa gactcgggga cctacacctg caatgtgacg gagagtgtga 961 atgaccatca ggatgaaaag gccatcaaca tcaccgtggt tgagagcggc tacgtgcggc 1021 tcctgggaga ggtgggcaca ctacaatttg ctgagctgca tcggagccgg acactgcagg 1081 tagtgttcga ggcctaccca ccgcccactg tcctgtggtt caaagacaac cgcaccctgg 1141 gcgactccag cgctggcgaa atcgccctgt ccacgcgcaa cgtgtcggag acccggtatg 1201 tgtcagagct gacactggtt cgcgtgaagg tggcagaggc tggccactac accatgcggg 1261 ccttccatga ggatgctgag gtccagctct ccttccagct acagatcaat gtccctgtcc 1321 gagtgctgga gctaagtgag agccaccctg acagtgggga acagacagtc cgctgtcgtg 1381 gccggggcat gccccagccg aacatcatct ggtctgcctg cagagacctc aaaaggtgtc 1441 cacgtgagct gccgcccacg ctgctgggga acagttccga agaggagagc cagctggaga 1501 ctaacgtgac gtactgggag gaggagcagg agtttgaggt ggtgagcaca ctgcgtctgc 1561 agcacgtgga tcggccactg tcggtgcgct gcacgctgcg caacgctgtg ggccaggaca 1621 cgcaggaggt catcgtggtg ccacactcct tgccctttaa ggtggtggtg atctcagcca 1681 tcctggccct ggtggtgctc accatcatct cccttatcat cctcatcatg ctttggcaga 1741 agaagccacg ttacgagatc cgatggaagg tgattgagtc tgtgagctct gacggccatg 1801 agtacatcta cgtggacccc atgcagctgc cctatgactc cacgtgggag ctgccgcggg 1861 accagcttgt gctgggacgc accctcggct ctggggcctt tgggcaggtg gtggaggcca 1921 cggctcatgg cctgagccat tctcaggcca cgatgaaagt ggccgtcaag atgcttaaat 1981 ccacagcccg cagcagtgag aagcaagccc ttatgtcgga gctgaagatc atgagtcacc 2041 ttgggcccca cctgaacgtg gtcaacctgt tgggggcctg caccaaagga ggacccatct 2101 atatcatcac tgagtactgc cgctacggag acctggtgga ctacctgcac cgcaacaaac 2161 acaccttcct gcagcaccac tccgacaagc gccgcccgcc cagcgcggag ctctacagca 2221 atgctctgcc cgttgggctc cccctgccca gccatgtgtc cttgaccggg gagagcgacg 2281 gtggctacat ggacatgagc aaggacgagt cggtggacta tgtgcccatg ctggacatga 2341 aaggagacgt caaatatgca gacatcgagt cctccaacta catggcccct tacgataact 2401 acgttccctc tgcccctgag aggacctgcc gagcaacttt gatcaacgag tctccagtgc 2461 taagctacat ggacctcgtg ggcttcagct accaggtggc caatggcatg gagtttctgg 2521 cctccaagaa ctgcgtccac agagacctgg cggctaggaa cgtgctcatc tgtgaaggca 2581 agctggtcaa gatctgtgac tttggcctgg ctcgagacat catgcgggac tcgaattaca 2641 tctccaaagg cagcaccttt ttgcctttaa agtggatggc tccggagagc atcttcaaca 2701 gcctctacac caccctgagc gacgtgtggt ccttcgggat cctgctctgg gagatcttca 2761 ccttgggtgg caccccttac ccagagctgc ccatgaacga gcagttctac aatgccatca 2821 aacggggtta ccgcatggcc cagcctgccc atgcctccga cgagatctat gagatcatgc 2881 agaagtgctg ggaagagaag tttgagattc ggcccccctt ctcccagctg gtgctgcttc 2941 tcgagagact gttgggcgaa ggttacaaaa agaagtacca gcaggtggat gaggagtttc 3001 tgaggagtga ccacccagcc atccttcggt cccaggcccg cttgcctggg ttccatggcc 3061 tccgatctcc cctggacacc agctccgtcc tctatactgc cgtgcagccc aatgagggtg 3121 acaacgacta tatcatcccc ctgcctgacc ccaaacccga ggttgctgac gagggcccac 3181 tggagggttc ccccagccta gccagctcca ccctgaatga agtcaacacc tcctcaacca 3241 tctcctgtga cagccccctg gagccccagg acgaaccaga gccagagccc cagcttgagc 3301 tccaggtgga gccggagcca gagctggaac agttgccgga ttcggggtgc cctgcgcctc 3361 gggcggaagc agaggatagc ttcctgttgc caactttctt gtacaaagtg gttggtaagc 3421 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 3481 ggcgcgttaa gtcgacaatc aacctctgga tta