Construct: ORF ccsbBroad304_14785
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018812.1_s304c1, BRDN0000459296
- Derived from:
- ccsbBroadEn_14785
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PRKAR1B (5575)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14785
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164758.1 | 99.3% | 33.1% | (many diffs) |
| 2 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164759.1 | 99.3% | 33.1% | (many diffs) |
| 3 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164760.2 | 99.3% | 33.1% | (many diffs) |
| 4 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164761.1 | 99.3% | 33.1% | (many diffs) |
| 5 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_001164762.1 | 99.3% | 33.1% | (many diffs) |
| 6 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | NM_002735.2 | 99.3% | 33.1% | (many diffs) |
| 7 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | XM_011515446.2 | 84.9% | 18% | (many diffs) |
| 8 | human | 5575 | PRKAR1B | protein kinase cAMP-depende... | XM_017012433.1 | 61.8% | 4.2% | (many diffs) |
| 9 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | NM_001253890.1 | 85.3% | 28.7% | (many diffs) |
| 10 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | NM_008923.3 | 85.3% | 28.7% | (many diffs) |
| 11 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504660.3 | 85.3% | 28.7% | (many diffs) |
| 12 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504661.3 | 85.3% | 28.7% | (many diffs) |
| 13 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_006504663.2 | 85.3% | 28.7% | (many diffs) |
| 14 | mouse | 19085 | Prkar1b | protein kinase, cAMP depend... | XM_017320719.1 | 85.3% | 28.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 498
- ORF length:
- 429
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcctccccg cccgcctgcc cctcggagga ggacgagagc ctgaagggct 121 gtgagctgta cgtgcagctg cacgggatcc agcaggtcct caaagactgt atcgtccacc 181 tctgcatctc caagcccgaa cgccccatga agttcctccg ggagcacttc gagaagctgg 241 agaaggaaga aaacaggcag attttggcgc ggcaaaagtc aaactcacag tcggactccc 301 atgatgagga ggtgtcgccc acccccccga accctgtggt gaaggcccgc cgccggcgag 361 gaggcgtgag tgccgaggtg tacaccgagg aggacgccgn gtcctacgtc aggaaaggtg 421 attccccaag gactacaaaa accatgactg cgctggccca aggccatctc caagaacgtg 481 ctcttcgctc acctggatga caacgagagg agtgacatat tcgatgccat gttccctgtc 541 actcacatcg ctggggagac tgttatacag caagggaatg aaggagacaa cttctatgtc 601 gttgatcaag gggaagtgga tgtgtacgtg aacggagagt gggtgaccaa catcagcgag 661 ggaggcagct tcggggagct ggcgctcatc tacggcaccc ccagggctgc gaccgtgaaa 721 gccaagacgg acctcaagct ctgggggatc gaccgggaca gctaccggcg catccttatg 781 ggcagcacgc tgaggaaacg caagatgtac gaggagttcc tcagcaaggt ctccatccta 841 gagtccctgg gagaagtggg agcgtctgac cgtggcggat gcgctgggag cccgtccagt 901 ttgaagatgg agagaaaatt gtggtccagg gagagcctgg ggacgacttt tacatcatca 961 cggagggcac cgcgtccgtg ctgcagcgcc ggtcccccaa tgaggagtac gtggaggtgg 1021 ggcgcctggg accctctgac tacttcgggg agattgcact gctgctgaac cggccccggg 1081 cggccactgt cgtggcccgg gggcccctca agtgtgtgaa gctggaccgg ccccgcttcg 1141 agcgtgtgct ggggccctgc tctgagatcc tcaagaggaa cattcagcgt tacaacagct 1201 tcatctccct caccgtcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 1261 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1321 agtcgacaat caacctctgg atta