Construct: ORF ccsbBroad304_14795

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018860.1_s304c1, BRDN0000459344
Derived from:
ccsbBroadEn_14795
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRKCQ (5588)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14795
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5588 PRKCQ protein kinase C theta NM_001323265.1 99.8% 31.5% (many diffs)
2 human 5588 PRKCQ protein kinase C theta NM_006257.5 99.8% 31.5% (many diffs)
3 human 5588 PRKCQ protein kinase C theta XM_024448076.1 99.8% 31.5% (many diffs)
4 human 5588 PRKCQ protein kinase C theta XM_005252496.4 95.2% 30.1% (many diffs)
5 human 5588 PRKCQ protein kinase C theta NM_001282644.2 94.7% 25.9% (many diffs)
6 human 5588 PRKCQ protein kinase C theta NM_001242413.2 90.8% 34.6% (many diffs)
7 human 5588 PRKCQ protein kinase C theta XM_005252497.4 89% 31.9% (many diffs)
8 human 5588 PRKCQ protein kinase C theta NM_001323267.1 88.2% 27.6% (many diffs)
9 human 5588 PRKCQ protein kinase C theta NM_001282645.1 82.1% 13.7% (many diffs)
10 human 5588 PRKCQ protein kinase C theta NM_001323266.2 82.1% 13.7% (many diffs)
11 human 5588 PRKCQ protein kinase C theta XM_024448077.1 82.1% 13.7% (many diffs)
12 mouse 18761 Prkcq protein kinase C, theta NM_008859.2 87% 30.8% (many diffs)
13 mouse 18761 Prkcq protein kinase C, theta XM_006497398.3 81.9% 25% (many diffs)
14 mouse 18761 Prkcq protein kinase C, theta XM_011238965.2 72.6% 13.4% (many diffs)
15 mouse 18761 Prkcq protein kinase C, theta XM_006497399.2 55.9% 1.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
753
ORF length:
684
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcgccattt cttcggattg gcttgtccaa ctttgactgc gggtcctgcc
121agtcttgtca gggcgaggct gttaaccctt actgtgctgt gctcgtcaaa gagtatgtcg
181aatcagagaa cgggcagatg tatatccaga aaaagcctac catgtaccca ccctgggaca
241gcacttttga tgcccatatc aacaagggaa gagtcatgca gatcattgtg aaaggcaaaa
301acgtggacct catctctgaa accaccgtgg agctctactc gctggctgag aggtgcagga
361agaacaacgg gaagacagaa atatggttag agctgaaacc tcaaggccga atgctaatga
421atgcaagata ctttctggaa atgagtgaca caaaggacat gaatgaattt gagacggaag
481gcttctttgc tttgcatcag cgccggggtg ccatcaagca ggcaaaggtc caccacgtca
541agtgccacga gttcactgcc accttcttcc cacagcccac attttgctct gtctgccacg
601agtttgtctg gggcctgaac aaacagggct accagtgccg acaatgcaat gcagcaattc
661acaagaagtg tattgataaa gttatagcaa agtgcacagg atcagctatc aatagccgag
721aaaccatgtt ccacaaagga gagattcaaa attgacatgc cacacagatt taaagtctac
781aattacaaga gcccgacctt ctgtgaacac tgtgggaccc tgctgtgggg actggcacgg
841caaggactca agtgtgatgc atgtggcatg aatgtgcatc atagatgcca gacaaaggtg
901gccaaccttt gtggcataaa ccagaagcta atggctgaag cgctggccat gattgagagc
961actcaacagg ctcgctgctt aagagatact gaacagatct tcagagaagg tccggttgaa
1021attggtctcc catgctccat caaaaatgaa gcaaggccgc catgtttacc gacaccggga
1081aaaagagagc ctcagggcat ttcctgggag tctccgttgg atgaggtgga taaaatgtgc
1141catcttccag aacctgaact gaacaaagaa agaccatctc tgcagattaa actaaaaatt
1201gaggatttta tcttgcacaa aatgttgggg aaaggaagtt ttggcaaggt cttcctggca
1261gaattcaaga aaaccaatca atttttcgca ataaaggcct taaagaaaga tgtggtcttg
1321atggacgatg atgttgagtg cacgatggta gagaagagag ttctttcctt ggcctgggag
1381catccgtttc tgacgcacat gttttgtaca ttccagacca aggaaaacct ctttttttgt
1441gatggagtac ctcaacggag gggacttaat gtaccacatc caaagctgcc acaagttcga
1501cctttccaga gcgacgtttt atgctgctga aatcattctt ggtctgcagt tccttcattc
1561caaaggaata gtctacaggg acctgaagct agataacatc ctgttagaca aagatggaca
1621tatcaagatt gcggattttg gaatgtgcaa ggagaacatg ttaggagatg ccaagacgaa
1681taccttctgt gggacacctg actacatcgc cccagagatc ttgctgggtc agaaatacaa
1741ccactctgtg gactggtggt ccttcggggt tctcctttat gaaatgctga ttggtcagtc
1801gcctttccac gggcaggatg aggaggagct cttccactcc atccgcatgg acaatccctt
1861ttacccacgg tggctggaga aggaagcaaa ggaccttctg gtgaagctct tcgtgagaga
1921acctgagaag aggctgggcg tgaggggaga catccgccag caccctttgt ttcgggagat
1981caactgggag gaacttgaac ggaaggagat tgacccaccg ttccggccga aagtgaaatc
2041accatttgac tgcagcaatt tcgacaaaga attcttaaac gagaagcccc ggctgtcatt
2101tgccgacaga gcactgatca acagcatgga ccagaatatg ttcaggaact tttccttcat
2161gaaccccggg atggagcggc tgatatcctt gccaactttc ttgtacaaag tggttggtaa
2221gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg
2281gtggcgcgtt aagtcgacaa tcaacctctg gatta

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