Construct: ORF ccsbBroad304_14820
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019057.1_s304c1, BRDN0000459856
- Derived from:
- ccsbBroadEn_14820
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTK7 (5754)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14820
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_002821.5 | 100% | 100% | |
| 2 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_001270398.1 | 96.9% | 96.5% | (many diffs) |
| 3 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_152880.4 | 96.2% | 96.2% | 1497_1498ins120 |
| 4 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_152882.4 | 94.7% | 94.7% | 1878_1879ins168 |
| 5 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | XM_011514765.2 | 93.2% | 92.9% | (many diffs) |
| 6 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NM_152881.4 | 87.8% | 87.7% | 1228_1229ins390 |
| 7 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | XM_011514766.2 | 85% | 84.5% | (many diffs) |
| 8 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NR_072997.2 | 71.3% | (many diffs) | |
| 9 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | NR_072998.2 | 70.5% | 1_175del;2581_2582ins233;3153_3989del | |
| 10 | human | 5754 | PTK7 | protein tyrosine kinase 7 (... | XR_926286.2 | 70.3% | (many diffs) | |
| 11 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | NM_175168.4 | 87.5% | 91.8% | (many diffs) |
| 12 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_006524922.2 | 86.6% | 90.2% | (many diffs) |
| 13 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_006524924.2 | 86.2% | 90.7% | (many diffs) |
| 14 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_006524923.1 | 85.7% | 89.3% | (many diffs) |
| 15 | mouse | 71461 | Ptk7 | PTK7 protein tyrosine kinase 7 | XM_017317638.1 | 68.2% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3279
- ORF length:
- 3210
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gggagctgcg cggggatccc cggccagacc ccgccggttg cctctgctca 121 gcgtcctgct gctgccgctg ctgggcggta cccagacagc cattgtcttc atcaagcagc 181 cgtcctccca ggatgcactg caggggcgcc gggcgctgct tcgctgtgag gttgaggctc 241 cgggcccggt acatgtgtac tggctgctcg atggggcccc tgtccaggac acggagcggc 301 gtttcgccca gggcagcagc ctgagctttg cagctgtgga ccggctgcag gactctggca 361 ccttccagtg tgtggctcgg gatgatgtca ctggagaaga agcccgcagt gccaacgcct 421 ccttcaacat caaatggatt gaggcaggtc ctgtggtcct gaagcatcca gcctcggaag 481 ctgagatcca gccacagacc caggtcacac ttcgttgcca cattgatggg caccctcggc 541 ccacctacca atggttccga gatgggaccc ccctttctga tggtcagagc aaccacacag 601 tcagcagcaa ggagcggaac ctgacgctcc ggccagctgg tcctgagcat agtgggctgt 661 attcctgctg cgcccacagt gcttttggcc aggcttgcag cagccagaac ttcaccttga 721 gcattgctga tgaaagcttt gccagggtgg tgctggcacc ccaggacgtg gtagtagcga 781 ggtatgagga ggccatgttc cattgccagt tctcagccca gccacccccg agcctgcagt 841 ggctctttga ggatgagact cccatcacta accgcagtcg ccccccacac ctccgcagag 901 ccacagtgtt tgccaacggg tctctgctgc tgacccaggt ccggccacgc aatgcaggga 961 tctaccgctg cattggccag gggcagaggg gcccacccat catcctggaa gccacacttc 1021 acctagcaga gattgaagac atgccgctat ttgagccacg ggtgtttaca gctggcagcg 1081 aggagcgtgt gacctgcctt ccccccaagg gtctgccaga gcccagcgtg tggtgggagc 1141 acgcgggagt ccggctgccc acccatggca gggtctacca gaagggccac gagctggtgt 1201 tggccaatat tgctgaaagt gatgctggtg tctacacctg ccacgcggcc aacctggctg 1261 gtcagcggag acaggatgtc aacatcactg tggccactgt gccctcctgg ctgaagaagc 1321 cccaagacag ccagctggag gagggcaaac ccggctactt ggattgcctg acccaggcca 1381 caccaaaacc tacagttgtc tggtacagaa accagatgct catctcagag gactcacggt 1441 tcgaggtctt caagaatggg accttgcgca tcaacagcgt ggaggtgtat gatgggacat 1501 ggtaccgttg tatgagcagc accccagccg gcagcatcga ggcgcaagcc cgtgtccaag 1561 tgctggaaaa gctcaagttc acaccaccac cccagccaca gcagtgcatg gagtttgaca 1621 aggaggccac ggtgccctgt tcagccacag gccgagagaa gcccactatt aagtgggaac 1681 gggcagatgg gagcagcctc ccagagtggg tgacagacaa cgctgggacc ctgcattttg 1741 cccgggtgac tcgagatgac gctggcaact acacttgcat tgcctccaac gggccgcagg 1801 gccagattcg tgcccatgtc cagctcactg tggcagtttt tatcaccttc aaagtggaac 1861 cagagcgtac gactgtgtac cagggccaca cagccctact gcagtgcgag gcccaggggg 1921 accccaagcc gctgattcag tggaaaggca aggaccgcat cctggacccc accaagctgg 1981 gacccaggat gcacatcttc cagaatggct ccctggtgat ccatgacgtg gcccctgagg 2041 actcaggccg ctacacctgc attgcaggca acagctgcaa catcaagcac acggaggccc 2101 ccctctatgt cgtggacaag cctgtgccgg aggagtcgga gggccctggc agccctcccc 2161 cctacaagat gatccagacc attgggttgt cggtgggtgc cgctgtggcc tacatcattg 2221 ccgtgctggg cctcatgttc tactgcaaga agcgctgcaa agccaagcgg ctgcagaagc 2281 agcccgaggg cgaggagcca gagatggaat gcctcaacgg tgggcctttg cagaacgggc 2341 agccctcagc agagatccaa gaagaagtgg ccttgaccag cttgggctcc ggccccgcgg 2401 ccaccaacaa acgccacagc acaagtgata agatgcactt cccacggtct agcctgcagc 2461 ccatcaccac gctggggaag agtgagtttg gggaggtgtt cctggcaaag gctcagggct 2521 tggaggaggg agtggcagag accctggtac ttgtgaagag cctgcagagc aaggatgagc 2581 agcagcagct ggacttccgg agggagttgg agatgtttgg gaagctgaac cacgccaacg 2641 tggtgcggct cctggggctg tgccgggagg ctgagcccca ctacatggtg ctggaatatg 2701 tggatctggg agacctcaag cagttcctga ggatttccaa gagcaaggat gaaaaattga 2761 agtcacagcc cctcagcacc aagcagaagg tggccctatg cacccaggta gccctgggca 2821 tggagcacct gtccaacaac cgctttgtgc ataaggactt ggctgcgcgt aactgcctgg 2881 tcagtgccca gagacaagtg aaggtgtctg ccctgggcct cagcaaggat gtgtacaaca 2941 gtgagtacta ccacttccgc caggcctggg tgccgctgcg ctggatgtcc cccgaggcca 3001 tcctggaggg tgacttctct accaagtctg atgtctgggc cttcggtgtg ctgatgtggg 3061 aagtgtttac acatggagag atgccccatg gtgggcaggc agatgatgaa gtactggcag 3121 atttgcaggc tgggaaggct agacttcctc agcccgaggg ctgcccttcc aaactctatc 3181 ggctgatgca gcgctgctgg gccctcagcc ccaaggaccg gccctccttc agtgagattg 3241 ccagcgccct gggagacagc accgtggaca gcaagccgtt gccaactttc ttgtacaaag 3301 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 3361 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta