Construct: ORF ccsbBroad304_14888

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018846.1_s304c1, BRDN0000459330
Derived from:
ccsbBroadEn_14888
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
BRD3 (8019)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14888
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8019 BRD3 bromodomain containing 3 XM_017015165.2 75% 33.1% (many diffs)
2 human 8019 BRD3 bromodomain containing 3 NM_007371.4 74.9% 33% (many diffs)
3 human 8019 BRD3 bromodomain containing 3 XM_006717291.3 74.9% 33% (many diffs)
4 human 8019 BRD3 bromodomain containing 3 XM_011519052.2 74.9% 33% (many diffs)
5 human 8019 BRD3 bromodomain containing 3 XM_017015166.2 31.1% 13.8% (many diffs)
6 mouse 67382 Brd3 bromodomain containing 3 NM_001113573.1 66.5% 31.6% (many diffs)
7 mouse 67382 Brd3 bromodomain containing 3 NM_023336.4 66.5% 31.6% (many diffs)
8 mouse 67382 Brd3 bromodomain containing 3 NM_001113574.1 65% 30.9% (many diffs)
9 mouse 67382 Brd3 bromodomain containing 3 XM_006498249.3 65% 30.9% (many diffs)
10 mouse 67382 Brd3 bromodomain containing 3 XM_006498250.2 65% 30.9% (many diffs)
11 mouse 67382 Brd3 bromodomain containing 3 XM_011239155.2 65% 30.9% (many diffs)
12 mouse 67382 Brd3 bromodomain containing 3 XM_011239156.2 65% 30.9% (many diffs)
13 mouse 67382 Brd3 bromodomain containing 3 XM_011239157.2 65% 30.9% (many diffs)
14 mouse 67382 Brd3 bromodomain containing 3 XM_011239158.2 65% 30.9% (many diffs)
15 mouse 67382 Brd3 bromodomain containing 3 XM_017319221.1 28.1% 13.5% (many diffs)
16 mouse 67382 Brd3 bromodomain containing 3 XM_011239159.2 27.4% 13.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
792
ORF length:
723
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtccaccgcc acgacagtcg cccccgcggg gatcccggcg accccgggcc
121ctgtgaaccc accccccccg gaggtctcca accccagcaa gcccggccgc aagaccaacc
181agctgcagta catgcagaat gtggtggtga agacgctctg gaaacaccag ttcgcctggc
241ccttctacca gcccgtggac gcaatcaaat tgaacctgcc ggattatcat aaaataatta
301aaaacccaat ggatatgggg actattaaga agagactaga aaataattat tattggagtg
361caagcgaatg tatgcaggac ttcaacacca tgtttacaaa ttgttacatt tataacaagc
421ccacagatga catagtgcta atggcccaag ctttagagaa aatttttcta caaaaagtgg
481cccagatgcc ccaagaggaa gttgaattat taccccctgc tccaaagggc aaaggtcgga
541agccggctgc gggagcccag agcgcaggta cacagcaagt ggcggccgtg tcctctgtct
601ccccagcgac cccctttcag agcgtgcccc ccaccgtctc ccagacgccc gtcatcgctg
661ccacccctgt accaaccatc actgcaaacg tcacgtcggt cccagtcccc ccagctgccg
721ccccacctcc tcctgccaca cccatcgtcc ccgtggtccc tcctacgccg cctgtcgtca
781agaaaaaggc gtgaagcgga aagcagacac aaccactccc acgacgtcgg ccatcactgc
841cagccggagt gagtcgcccc cgccgttgtc agaccccaag caggccaaag tggtggcccg
901gcgggagagt ggtggccgcc ccatcaagcc tcccaagaag gacctggaga acggccgagg
961tgccccagca cgccaggcaa gaagggcaag ctgtcggagc acctgcgcta ctgcgacagc
1021atcctcaggg agatgctatc caagaagcac gcggctacgc ctggccttct acaagccagt
1081ggatgccgag ggcctggagc tgcacgacta ccacgacatc atcaagcacc cgatggacct
1141cagcaccgtg aaaaggaaga tggatggccg agagtaccca gacgcacagg gctttgctgc
1201tgatgtccgg ctgatgttct cgaattgcta caaatacaat cccccagacc acgaggttgt
1261ggccatggcc cggaagctcc aggacgtgtt tgagatgagg tttgccaaga tgccagatga
1321gcccgtggag gcaccggcgc tgcctgcccc tgcggccccc atggtgagca agggcgctga
1381gagcagccgt agcagtgagg agagctcttc ggactcaggc agctcggact cggaggagga
1441gcgggccacc aggctggcgg agctgcagga gcagctgaag gccgtgcacg agcagctggc
1501cgccctgtct caggccccag taaacaaacc aaagaagaag aaggagaaga aggagaagga
1561gaagaagaag aaggacaagg agaaggagaa ggagaagcac aaagtgaagg ccgaggaaga
1621gaagaaggcc aaggtggctc cgcctgccaa gcaggctcag cagaagaagg ctcctgccaa
1681gaaggccaac agcacgacca cggccggcag agatcatttc ttgacctgtg gagttttgcc
1741aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc
1801tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat
1861ta

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