Construct: ORF ccsbBroad304_14906

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018906.1_s304c1, BRDN0000459390
Derived from:
ccsbBroadEn_14906
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CASK (8573)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14906
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8573 CASK calcium/calmodulin dependen... NM_001126054.2 98.5% 26.1% (many diffs)
2 human 8573 CASK calcium/calmodulin dependen... NM_001126055.2 98.4% 26.1% (many diffs)
3 human 8573 CASK calcium/calmodulin dependen... XM_011543995.2 97.7% 25.9% (many diffs)
4 human 8573 CASK calcium/calmodulin dependen... XM_006724566.3 97% 26.5% (many diffs)
5 human 8573 CASK calcium/calmodulin dependen... NM_001367721.1 96.6% 25.3% (many diffs)
6 human 8573 CASK calcium/calmodulin dependen... XM_011543996.2 96.4% 26.2% (many diffs)
7 human 8573 CASK calcium/calmodulin dependen... XM_011543994.2 96.1% 25.6% (many diffs)
8 human 8573 CASK calcium/calmodulin dependen... NM_003688.3 96.1% 25.5% (many diffs)
9 human 8573 CASK calcium/calmodulin dependen... XM_005272686.4 95.9% 25.5% (many diffs)
10 human 8573 CASK calcium/calmodulin dependen... XM_011543993.2 95.3% 25.2% (many diffs)
11 human 8573 CASK calcium/calmodulin dependen... XM_024452473.1 76.6% 5.6% (many diffs)
12 human 8573 CASK calcium/calmodulin dependen... XM_011543997.3 76% 5.4% (many diffs)
13 mouse 12361 Cask calcium/calmodulin-dependen... XM_006527561.3 93.7% 26.1% (many diffs)
14 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318369.1 93.6% 25.7% (many diffs)
15 mouse 12361 Cask calcium/calmodulin-dependen... NM_009806.3 93.5% 26.1% (many diffs)
16 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318370.1 93.1% 25.8% (many diffs)
17 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247441.2 92.9% 25.9% (many diffs)
18 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318371.1 92.3% 26% (many diffs)
19 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247440.2 92.2% 25.8% (many diffs)
20 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247439.2 92.1% 25.4% (many diffs)
21 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284503.1 91.9% 25.3% (many diffs)
22 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284504.1 91.7% 26.7% (many diffs)
23 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318368.1 91.6% 25.5% (many diffs)
24 mouse 12361 Cask calcium/calmodulin-dependen... XM_006527560.3 91.4% 25.5% (many diffs)
25 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247438.2 91.3% 25.1% (many diffs)
26 mouse 12361 Cask calcium/calmodulin-dependen... NM_001284505.1 91.2% 25.5% (many diffs)
27 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318367.1 90.8% 25.2% (many diffs)
28 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247436.2 89.6% 24.6% (many diffs)
29 mouse 12361 Cask calcium/calmodulin-dependen... XM_017318366.1 89% 24.8% (many diffs)
30 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247437.1 88.4% 24.9% (many diffs)
31 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247435.2 88.2% 24.3% (many diffs)
32 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247433.1 88.1% 24.3% (many diffs)
33 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247431.1 87.5% 24% (many diffs)
34 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247432.1 87.1% 24.1% (many diffs)
35 mouse 12361 Cask calcium/calmodulin-dependen... XM_011247434.1 86.9% 24.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
777
ORF length:
708
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggccgacgac gacgtgctgt tcgaggatgt gtacgagctg tgcgaggtga
121tcggaaaggg tcccttcagt gttgtacgac gatgtatcaa cagagaaact gggcaacaat
181ttgctgtaaa aattgttgat gtagccaagt tcacatcaag tccagggtta agtacagaag
241atctaaagcg ggaagccagt atctgtcata tgctgaaaca tccacacatt gtagagttat
301tggagacata tagctcagat ggaatgcttt acatggtttt cgaatttatg gatggagcag
361atctgtgttt tgaaatcgta aagcgagctg acgctggttt tgtgtacagt gaagctgtag
421ccagccatta tatgagacag atactggaag ctctacgcta ctgccatgat aataacataa
481ttcacaggga tgtgaagccc cactgtgttc tccttgcctc aaaagaaaac tcggcacctg
541ttaaacttgg aggctttggg gtagctattc aattagggga gtctggactt gtagctggag
601gacgtgttgg aacacctcat tttatggcac cagaagtggt caaaagagag ccttacggaa
661agcctgtaga cgtctggggg tgcggtgtga tcctttttat cctgctcagt ggttgtttgc
721ctttttacgg aaccaaggaa agattgtttg aaggcattat taaaggaaaa tatagatgaa
781tccaaggcag tggagccata tctctgaaag tgccaagacc tagtacgtcg catgctgatg
841ctggatccag ctgaaaggat ccactgtttt atgaagccac tgaatcaccc atggcttaag
901gagcgggatc gttacgccta caagattcat cttccagaaa cagtagagca gctgaggaaa
961ttcaatgcaa ggaggaaact aaagggtgca gtactagccg ctgtgtcaag tcacaaattc
1021aactcattct atggggatcc ccctgaagag ttaccagatt tctccgaaga ccctacctcc
1081tcaggacttc tagcagcaga aagagcagtc tcacaggtgc tggacagcct ggaagagatt
1141catgcgctta cagactgcag tgaaaaggac ctagattttc tacacagtgt tttccaggat
1201cagcatcttc acacactact agatctgtat gacaaaatta acacaaagtc ttcaccacaa
1261atcaggaatc ctccaagcga tgcagtacag agagccaaag aggtattgga agaaatttca
1321tgttaccctg agaataacga cgcaaaggaa ctaaagcgta ttttaacaca acctcatttc
1381atggccttac ttcagactca cgacgtagtg gcacatgaag tttacagtga tgaagcattg
1441agggtcacac ctcctcccac ctctccctat ttaaacggcg attctccaga aagtgctaac
1501ggagacatgg atatggagaa tgtgaccaga gttcggctgg tacagtttca aaagaacaca
1561gatgaaccaa tgggaatcac tttaaaaatg aatgaactaa atcattgtat tgttgcaaga
1621attatgcatg ggggcatgat tcacaggcaa ggtacacttc atgttggtga tgaaattcga
1681gaaatcaatg gcatcagtgt ggctaaccaa acagtggaac aactgcaaaa aatgcttagg
1741gaaatgcggg ggagtattac cttcaagatt gtgccaagtt accgcactca gtcttcgtcc
1801tgtgaggact tgccatcaac tacccaacca aaaggacgac agatctatgt aagagcacaa
1861tttgaatatg atccagccaa ggatgacctc atcccctgta aagaagctgg cattcgattc
1921agagttggtg acatcatcca gattattagt aaggatgatc ataattggtg gcagggtaac
1981ctgaaaaact cccaaaaatt ggactgcagt ctcattcctt cttcctgact ttcaggaatt
2041gcgagtagct tgcatgncca tggagagacc caaacaggag cagcagccca gctgtacttg
2101tttgcaagaa aaaagaagca gtacaaagat aaatatttgg caaagcacaa tgcagtgttn
2161gatcaattag atcttgtcac atatgaagaa gtagtaaaac tgccagcatt caagaggaaa
2221acactagtct tattaggcgc acatggtgtt gggagaagac acataaaaaa cactctcatc
2281acaaagcacc cagaccggtt tgcgtaccct attccacata caaccagacc tccaaagaaa
2341gacgaagaaa atggaaagaa ttattacttt gtatctcatg accaaatgat gcaagacatc
2401tctaataacg agtacttgga gtacggcagc cacgaggatg cgatgtatgg gacaaaactg
2461gagaccatcc ggaagatcca cgagcagggg ctgattgcaa tactggacgt ggagcctcag
2521gcactgaagg tcctgagaac tgcagagttt gctccttttg ttgttttcat tgctgcacca
2581actattactc caggtttaaa tgaggatgaa tctcttcagc gtctgcagaa ggagtctgac
2641atcttacaga gaacatatgc acactacttc gatctcacaa ttatcaacaa tgaaattgat
2701gagacaatca gacatctgga ggaagctgtt gagctcgtgt gcacagcccc acagtgggtc
2761cctgtctcct gggtctattt gccaactttc ttgtacaaag tggttggtaa gcctatccct
2821aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt
2881aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)