Construct: ORF ccsbBroad304_14912
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018972.1_s304c1, BRDN0000459456
- Derived from:
- ccsbBroadEn_14912
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RIOK3 (8780)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14912
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8780 | RIOK3 | RIO kinase 3 | NM_003831.5 | 98.8% | 52.1% | (many diffs) |
| 2 | human | 8780 | RIOK3 | RIO kinase 3 | NM_001348193.2 | 95.7% | 49% | (many diffs) |
| 3 | human | 8780 | RIOK3 | RIO kinase 3 | XM_011526242.1 | 51.2% | 3.7% | (many diffs) |
| 4 | human | 8780 | RIOK3 | RIO kinase 3 | XM_011526243.1 | 49.7% | 5.5% | (many diffs) |
| 5 | human | 8780 | RIOK3 | RIO kinase 3 | NR_145477.2 | 42.8% | (many diffs) | |
| 6 | human | 8780 | RIOK3 | RIO kinase 3 | XR_002958189.1 | 33.9% | (many diffs) | |
| 7 | mouse | 66878 | Riok3 | RIO kinase 3 | NM_024182.4 | 88.9% | 49.2% | (many diffs) |
| 8 | mouse | 66878 | Riok3 | RIO kinase 3 | XM_017317969.1 | 34.5% | 58.2% | (many diffs) |
| 9 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782390.1 | 24.2% | (many diffs) | |
| 10 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782391.1 | 23% | (many diffs) | |
| 11 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782389.1 | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tctggtagga gtggcatcgc ctgagcccgg gacggcagcg gcctggggac 121 ccagcaagtg tccatgggct attcctcaaa atacaatatc ttgttctttg gctgatgtaa 181 tgagtgaaca gctggccaaa gaattgcagt tagaagaaga agctgccgtt tttcctgaag 241 ttgctgttgc tgaaggacca tttattactg gagaaaacat tgatacttcc agtgacctta 301 tgctggctca gatgctacag atggaatatg acagagaata tgatgcacag cttaggcgtg 361 aagaaaaaaa attcaatgga gatagcaaag tttccatttc ctttgaaaat tatcgaaaag 421 tgcatcctta tgaagacagc gatagctctg aagatgaggt tgactggcag gatactcgtg 481 atgatcccta cagaccagca aaaccggttc ccactcctaa aaagggcttt attggaaaag 541 gaaaagatat caccaccaaa catgatgaag tagtatgtgg gagaaagaac acagcaagaa 601 tggaaaattt tgcacctgag tttcaggtag gagatggaat tggaatggat ttaaaactat 661 caaaccatgt tttcaatgct ttaaaacaac atgcctactc agaagaacgt cgaagtgccc 721 gcctacatga gaaaaaggag cattctacag cagaaaaagc agttgatcct aagacacgtt 781 tacttatgta taaaatggtc aactctggaa tgttgagaca ntcacnggnt gttattagtn 841 cagnaaagnn ntcttgttgt tctttcatgc atatggaggg agcatggagg atgaaaagga 901 ngattgtaaa ggttatactt acagaatgtg ccatcaaggt atttaaaaca acccttaaat 961 gatttaagaa tcgtgacaaa tatattaaag atgatttcag gtttaaagat cgcttcagta 1021 aactaaatcc acgtaagatc atccgcatgt gggcagaaaa agaaatgcac aatctcgcaa 1081 gaatgcagag agctggaatt ccttgtccaa cagttgtact actgaagaaa cacattttag 1141 ttatgtcttt tattggccat gatcaagttc cagcccctaa attaaaagaa gtaaagctca 1201 atagtgaaga aatgaaagaa gcctactatc aaactcttca tttgatgcgg cagttatatc 1261 atgaatgtac gcttgtccat gctgacctca gtgagtataa catgctgtgg catgctggaa 1321 aggtctggtt gatcgatgtc agtcagtcag tagaacctac ccaccctcac ggcctggagt 1381 tcttgttccg ggactgcagg aatgtctcgc agtttttcca gaaaggagga gtcaaggaag 1441 cccttagtga acgagaactc ttcaatgctg tttcaggctt aaacatcaca gcagataatg 1501 aagctgattt tttagctgag atagaagctt tggagaaaat gaatgaagat cacgttcaga 1561 agaatggaag gaaagctgct tcatttttga aagatgatgg agacccacca ctactatatg 1621 atgaattgcc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1681 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1741 acctctggat ta