Construct: ORF ccsbBroadEn_14912
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018972.1_s300c1, BRDN0000458268
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RIOK3 (8780)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8780 | RIOK3 | RIO kinase 3 | NM_003831.5 | 98.8% | 52.1% | (many diffs) |
| 2 | human | 8780 | RIOK3 | RIO kinase 3 | NM_001348193.2 | 95.7% | 49% | (many diffs) |
| 3 | human | 8780 | RIOK3 | RIO kinase 3 | XM_011526242.1 | 51.2% | 3.7% | (many diffs) |
| 4 | human | 8780 | RIOK3 | RIO kinase 3 | XM_011526243.1 | 49.7% | 5.5% | (many diffs) |
| 5 | human | 8780 | RIOK3 | RIO kinase 3 | NR_145477.2 | 42.8% | (many diffs) | |
| 6 | human | 8780 | RIOK3 | RIO kinase 3 | XR_002958189.1 | 33.9% | (many diffs) | |
| 7 | mouse | 66878 | Riok3 | RIO kinase 3 | NM_024182.4 | 88.9% | 49.2% | (many diffs) |
| 8 | mouse | 66878 | Riok3 | RIO kinase 3 | XM_017317969.1 | 34.5% | 58.2% | (many diffs) |
| 9 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782390.1 | 24.2% | (many diffs) | |
| 10 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782391.1 | 23% | (many diffs) | |
| 11 | mouse | 66878 | Riok3 | RIO kinase 3 | XR_001782389.1 | 22.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 990
- ORF length:
- 894
- Sequence:
-
1 AACGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCATGGA TCTGGTAGGA GTGGCATCGC 121 CTGAGCCCGG GACGGCAGCG GCCTGGGGAC CCAGCAAGTG TCCATGGGCT ATTCCTCAAA 181 ATACAATATC TTGTTCTTTG GCTGATGTAA TGAGTGAACA GCTGGCCAAA GAATTGCAGT 241 TAGAAGAAGA AGCTGCCGTT TTTCCTGAAG TTGCTGTTGC TGAAGGACCA TTTATTACTG 301 GAGAAAACAT TGATACTTCC AGTGACCTTA TGCTGGCTCA GATGCTACAG ATGGAATATG 361 ACAGAGAATA TGATGCACAG CTTAGGCGTG AAGAAAAAAA ATTCAATGGA GATAGCAAAG 421 TTTCCATTTC CTTTGAAAAT TATCGAAAAG TGCATCCTTA TGAAGACAGC GATAGCTCTG 481 AAGATGAGGT TGACTGGCAG GATACTCGTG ATGATCCCTA CAGACCAGCA AAACCGGTTC 541 CCACTCCTAA AAAGGGCTTT ATTGGAAAAG GAAAAGATAT CACCACCAAA CATGATGAAG 601 TAGTATGTGG GAGAAAGAAC ACAGCAAGAA TGGAAAATTT TGCACCTGAG TTTCAGGTAG 661 GAGATGGAAT TGGAATGGAT TTAAAACTAT CAAACCATGT TTTCAATGCT TTAAAACAAC 721 ATGCCTACTC AGAAGAACGT CGAAGTGCCC GCCTACATGA GAAAAAGGAG CATTCTACAG 781 CAGAAAAAGC AGTTGATCCT AAGACACGTT TACTTATGTA TAAAATGGTC AACTCTGGAA 841 TGTTGAGACA NTCACNGGNT GTTATTAGTN CAGNAAAGNN NTCTTGTTGT TCTTTCATGC 901 ATATGGAGGG AGCATGGAGG ATGAAAAGGA NGATTGTAAA GGTTATACTT ACAGAATGTG 961 CCATCAAGGT ATTTAAAACA ACCCTTAAAT GATTTAAGAA TCGTGACAAA TATATTAAAG 1021 ATGATTTCAG GTTTAAAGAT CGCTTCAGTA AACTAAATCC ACGTAAGATC ATCCGCATGT 1081 GGGCAGAAAA AGAAATGCAC AATCTCGCAA GAATGCAGAG AGCTGGAATT CCTTGTCCAA 1141 CAGTTGTACT ACTGAAGAAA CACATTTTAG TTATGTCTTT TATTGGCCAT GATCAAGTTC 1201 CAGCCCCTAA ATTAAAAGAA GTAAAGCTCA ATAGTGAAGA AATGAAAGAA GCCTACTATC 1261 AAACTCTTCA TTTGATGCGG CAGTTATATC ATGAATGTAC GCTTGTCCAT GCTGACCTCA 1321 GTGAGTATAA CATGCTGTGG CATGCTGGAA AGGTCTGGTT GATCGATGTC AGTCAGTCAG 1381 TAGAACCTAC CCACCCTCAC GGCCTGGAGT TCTTGTTCCG GGACTGCAGG AATGTCTCGC 1441 AGTTTTTCCA GAAAGGAGGA GTCAAGGAAG CCCTTAGTGA ACGAGAACTC TTCAATGCTG 1501 TTTCAGGCTT AAACATCACA GCAGATAATG AAGCTGATTT TTTAGCTGAG ATAGAAGCTT 1561 TGGAGAAAAT GAATGAAGAT CACGTTCAGA AGAATGGAAG GAAAGCTGCT TCATTTTTGA 1621 AAGATGATGG AGACCCACCA CTACTATATG ATGAATTGCC AACTTTCTTG TACAAAGTTG 1681 GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA ACAGGTCACT ATCAGTCAAA 1741 ATAAAATCAT TATTGCCATC CCAGCCTGAT T