Construct: ORF ccsbBroad304_14945
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006273.1_s304c1, BRDN0000462429
- Derived from:
- ccsbBroadEn_14945
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- KNTC1 (9735)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14945
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9735 | KNTC1 | kinetochore associated 1 | NM_014708.6 | 99.8% | 43.5% | (many diffs) |
| 2 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_006719706.2 | 99.8% | 43.5% | (many diffs) |
| 3 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_011539027.3 | 98.7% | 44% | (many diffs) |
| 4 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_017020254.2 | 98.5% | 44.1% | (many diffs) |
| 5 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_017020255.2 | 93% | 36.6% | (many diffs) |
| 6 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_017020256.2 | 73.5% | 17.1% | (many diffs) |
| 7 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_011539029.3 | 70.2% | 61.8% | (many diffs) |
| 8 | human | 9735 | KNTC1 | kinetochore associated 1 | XR_944845.3 | 67.1% | (many diffs) | |
| 9 | human | 9735 | KNTC1 | kinetochore associated 1 | XR_944844.3 | 67% | (many diffs) | |
| 10 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_011539030.1 | 66.1% | 65.4% | (many diffs) |
| 11 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_024449285.1 | 65.7% | 66.1% | (many diffs) |
| 12 | human | 9735 | KNTC1 | kinetochore associated 1 | XM_017020257.1 | 65.4% | 65.6% | (many diffs) |
| 13 | mouse | 208628 | Kntc1 | kinetochore associated 1 | XM_006530251.3 | 51.6% | 6.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2958
- ORF length:
- 2889
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtggaatgat attgagctgc taacaaatga tgataccgga agtgggtacc 121 tgagtgtcgg ttcaagaaaa gaacatggaa ctgctttata tcaagtagat ttgctagtga 181 agatctcttc tgaaaaggcc tcattaaatc caaagataca ggcatgcagc ttaagtgatg 241 ggtttattat tgtagccgac caatcagtga tattgcttga cagtatttgt agatcacttc 301 aattgcatct tgtctttgat actgaagtgg atgtagttgg cctttgtcaa gaaggaaagt 361 ttcttttggt tggcgagaga agtggcaacc tacatcttat tcatgtaaca tcaaaacaaa 421 cactactcac taatgcattt gttcagaaag ctaacgatga aaatcggcgg acttaccaga 481 atcttgtcat tgagaaggat ggttcaaatg aaggtaccta ttatatgcta cttcttacat 541 acagtggatt tttttgtatt acaaaccttc agcttttaaa aattcaacaa gcaattgaga 601 atgtagactt cagtacagca aaaaagttac aaggacaaat caagtccagt tttatttcta 661 ctgaaaatta tcatactctt ggttgtctca gtcttgtggc tggagattta gcaagtgaag 721 ttcctgtgat aattggggga accggtaatt gtgcattctc aaaatgggaa ccagattctt 781 ccaagaaagg aatgacagtt aagaacctta ttgatgcaga gattattaaa ggtgcaaaga 841 agttccagct gatagacaat ctactttttg ttcttgatac tgataacgtg ctgagtttat 901 gggatattta cactctaact cctgtatgga actggccctc tcttcacgta gaagagtttc 961 ttcttactac agaagcagac tctccttcat cagtcacgtg gcaaggaatt acaaatctca 1021 aattaatagc tctgacagct tcagctaata agaagatgaa aaacctcatg gtttattcat 1081 tacctacaat ggaaatacta tattctttgg aagtatctag tgtttcttct ctggtccaaa 1141 caggaattag cacagatacc atataccttt tagaaggagt ttgcaaaaat gatccaaaat 1201 tgtctgaaga ctcagtctct gtgttagtac tcagatgtct tacggaagct ttaccagaaa 1261 acagattgag tcggttactt cacaaacaca gatttgctga agctgagagt tttgccattc 1321 agtttggact agatgttgag cttgtttaca aggtcaagtc aaatcatata ttggagaaac 1381 tggcattgag ttctgtggat gccagtgaac agaccgaatg gcaacaactt gtagacgacg 1441 ctaaggaaaa tctacataag atccaggatg atgaatttgt ggtgaattac tgcctgaaag 1501 ctcagtggat aacctatgaa accactcaag agatgctgaa ttatgccaaa accaggcttt 1561 tgaagaaaga agataaaact gctctcattt attctgatgg cttgaaagag gtgctaagag 1621 ctcatgcaaa attgactact ttttatggag catttggacc agaaaaattc agtggcagtt 1681 cttggattga atttctaaat aatgaagatg atcttaaaga tattttttta cagctaaaag 1741 aaggaaacct tgtttgtgca cagtatcttt ggcttcgaca tcgggcaaac tttgaaagca 1801 gatttgatgt gaaaatgctg gagagcttgc tcaactcaat gtctgcatca gtctctttgc 1861 aaaagctgtg tccatggttt aaaaatgatg tgattccatt tgtaagaagg actgtgcctg 1921 aaggacagat aattcttgca aaatggttgg aacaagcagc caggaacctt gaattaactg 1981 ataaggcaaa ttggccagaa aatggacttc aattggcaga gatatttttt acagcagaaa 2041 aaacagacga gttgggattg gcatcttcct ggcattggat ttccttgaaa gattatcaga 2101 acacagagga agtatgtcag ctaaggactt tggtaaataa cttgcgagag ttgatcacgt 2161 tgcataggaa gtacaactgc aaattagccc tctctgattt tgagaaggaa aatacaacca 2221 ccatagtgtt ccgaatgttt gataaagtgc tggccccaga gcttattccc tccatcttag 2281 agaagtttat aagagtttac atgagagaac atgacttgca agaggaggaa cttctcttgc 2341 tgtacataga ggatttactg aatagatgca gctcaaagtc cacatcactc tttgaaacag 2401 catgggaagc aaaggccatg gcagtaatag cgtgtttatc tgacacggac ctcatatttg 2461 atgccgtgct caagatcatg tatgcggcag tggttccttg gagtgcagct gtggagcaac 2521 tggtgaaaca gcacctggaa atggaccatc ccaaagtcaa gttattacag gaaagttaca 2581 aactaatgga gatgaaaaaa cttttacgag gctatggaat aagagaggta aatctcttaa 2641 acaaggaaat aatgagagtg gttagataca ttctcaaaca agatgtccca tcttctttag 2701 aagatgcttt aaaggtagcc caagcgttta tgttatctga tgatgagatc tacagtctaa 2761 gaattattga cctgattgat agagaacagg gtgaagactg tctccttctg ttgaagtctt 2821 tgcctcctgc tgaagctgag aaaactgcag aaagagtcat catatgggca cgactggcat 2881 tacaagaaga gccagatcat tctaaagagg gcaaggcctg gagaatgtct gtagcgaaga 2941 catccgtgga tcattcttaa gatactatgt gacattcaga aagacaatct gcagaagaag 3001 gacgaaacgt gaaaaaatgt tgaaactatt taaagaggtt gctagcttac aggagaactt 3061 tgaggtcttt ctttcatttg aagattatag caatagttcc ctggtagcag atctccgtga 3121 gcagcacatt aaagctcacg aagttgcaca ggcgaaacac aaacctggga gcaccccaga 3181 gcccatagct gctgaggtga ggagcccaag catggaatca aagctgcaca gacaggcact 3241 ggccctgcag atgtccaaac aagagctgga ggcagagctg accttgagag ccttaaaaga 3301 tgggaacatc aaaacagcac tgaaaaaatg cagcgacttg tttaagtatc actgcaatgc 3361 tgacactggg aaattgctat ttctgacatg tcagaagctt tgtcagatgt tggctgataa 3421 tgtcccagtg acagtgcctg tgggactgaa tcttccttcc atgatacatg atctagcaag 3481 ccaagctgcc accatttgca gtccagattt tttactagat gctttagaac tatgtaaaca 3541 tactttaatg gctgtagagc tttccagaca atgccaaatg gatgactgtg gaatcctcat 3601 gaaagcttct tttgggacac ataaagatcc atatgaagag tggtcttaca gtgacttctt 3661 cagtgaagat ggaattgttc ttgagtcaca gatggtgctt ccagtgattt atgaactgat 3721 ttcatctctt gtgcctctag ctgaaagcaa gagatatccc ttggagtcta ccagtttgcc 3781 atactgctcc cttaatgaag gagatggcct tgttttacct gttataaatt ccatctctgc 3841 cctgcttcag aatcttcagg aatctagcca gtgggagcta gccctaagat ttgtggttgg 3901 ttcatttggt acctgtcttc agcactctgt gtcaaacttc atgaatgcca ctttgagtga 3961 aaagttattt ggagagacta cattagttaa atcaaggcat gttgttatgg aattgaaaga 4021 aaaagctgtt atatttatca gggaaaatgc tacaacacta ctgcacaaag tatttaattg 4081 tcgcttggta gatcttgacc tggcgttggg ttactgcact ctcttacctc aaaaagatgt 4141 gtttgaaaat ctctggaagc tcatagataa agcatggcag aattacgaca aaatcttggc 4201 aatatctctg gtgggctctg agctggcaag tctctatcag gaaatagaaa tggggcttaa 4261 gttccgtgaa ctcagtactg atgcccagtg gggcattcgt cttggtaaac ttggtatttc 4321 ttttcaacca gttttcaggc aacattttct caccaagaaa gacctcatta aagctcttgt 4381 ggagaatata gatatggaca caagcctcat tttggaatat tgcagcacat ttcagttgga 4441 ctgcgatgca gttcttcagc tcttcattga aacgctgctc cacaacacaa atgccggcca 4501 aggccaggga gatgcaagca tggactctgc aaagcggcgg catcccaaac tcctggccaa 4561 agcccttgag atggttcctt tactgacgag cacaaaagat ttggtcatca gtcttagtgg 4621 aatactacat aagctggatc cttatgacta tgaaatgatt gaagttgtct tgaaagttat 4681 agaacgagct gatgaaaaga taaccaatat taatattaat caggcattga gtattctgaa 4741 acatttgaag tcatacagaa gaatttctcc tcccgtggat ctagaatatc agtatatgtt 4801 ggaacatgtc ataactttgc catcagctgc ccaaactaga ctgccttttc acctgatatt 4861 ctttggcaca gcacagaact tctggaaaat tctctctaca gaactcagtg aagaatcttt 4921 cccaacattg ctcttaattt cgaaattaat gaagttctct ctggacactc tgtacgtgtc 4981 tacagcaaaa cacgttttcg aaaaaaaact gaagccaaag ctcctgaagt taacacaagc 5041 taaatcctca acactgatta acaaggaaat aactaagatc acgcagacca tcgaatcctg 5101 cttactctct atagtcaacc cagagtgggc tgtagctatt gccatcagcc ttgcccagga 5161 tatccctgaa ggttccttca agatatctgc tttgaaattc tgcctttatt tagctgagag 5221 atggctacag aatatcccat cgcaggacga aaaacgtgaa aaagccgagg ctttgttgaa 5281 gaagcttcnn atccagtacc ggcgatcggg cacagaagct gtgctcatag cccacaagct 5341 gaacactgag gaatatttaa gagtgatcgg aaagccagca catcttattg tcagtctcta 5401 cgaacatcct agcatcaatc aaagaattca gaattcatct ggcacagatt atcctgatat 5461 tcatgcagca gctaaagaaa tagccgaagt caatgaaatt aatttggaaa aagtctggga 5521 catgttgttg gaaaaatggc tatgcccttc aacaaaacct ggtgaaaaac catcagaatt 5581 atttgaactt caagaagatg aagccctacg aagagtgcag tatctcctcc tgtctcgtcc 5641 aattgattat agttcaagaa tgctgtttgt atttgcaaca tcaactacaa ccacattagg 5701 tatgcatcag ttaacttttg cccatagaac tcgagctctt cagtgtctct tctatttggc 5761 tgacaaggaa actatagaat ctctctttaa aaaacccatt gaagaagtga aatcttattt 5821 gagatgtata acttttctgg catcatttga gactttgaat atccccatca catatgaatt 5881 attttgcagc agtcctaaag aaggaatgat taagggtctg tggaaaaacc acagccacga 5941 gtccatggca gtaagattgg tgactgagct gtgtttagaa tacaaaatct atgacctgca 6001 gctttggaat ggactcttgc aaaagcttct gggcttcaat atgattcctt atctaaggaa 6061 agttttaaaa gccatctcca gtatccattc tttatggcag gttccctact tcagcaaagc 6121 gtggcagcgt gtgatacaga taccactgct ttcagcctct tgtcctttaa gtcctgatca 6181 gctgtcagat tgttctgaga gtctcatcgc tgtcctcgaa tgtccagtct caggtgatct 6241 tgacctgatc ggagtcgcca ggcagtatat ccagttagaa cttccggctt ttgcattagc 6301 ttgtctgatg ctcatgcccc actcagagaa aagacaccag caaattaaga attttctggg 6361 ttcctgtgac cctcaggtta ttttaaagca attggaagag catatgaaca cgggccagct 6421 agcaggattt tcacatcaaa ttagaagtct gattttgaat aatatcatca ataagaagga 6481 gtttgggatt ttggcaaaga ccaaatactt tcaaatgttg aagatgcatg cgatgaatac 6541 caacaatatc actgagctag tgaactattt ggcaaatgac ttaagtttag atgaagcttc 6601 agtcttgata actgaatatt caaagcactg cgggaaacct gtgcctccag acactgctcc 6661 ctgtgaaatt ctgaaatgtt tcttagtgga ttatcgttgc caactttctt gtacaaagtg 6721 gttggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc 6781 gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga tta