Construct: ORF ccsbBroad304_14996

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014115.2_s304c1, BRDN0000459760
Derived from:
ccsbBroadEn_14996
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AAK1 (22848)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14996
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22848 AAK1 AP2 associated kinase 1 NM_001371577.1 68.7% 68.9% 1396_1397insATACAATAA;1400_1401insTAGTATGAAA;1404_2022delinsT
2 human 22848 AAK1 AP2 associated kinase 1 NM_001371575.1 50.3% 50.4% 1396_1397insATACAATAA;1400_1401insTAGTATGAAA;1404_2766delinsT
3 human 22848 AAK1 AP2 associated kinase 1 NM_014911.4 48.3% 48.4% 1396_1397insATACAATAA;1400_1401insTAGTATGAAA;1404_2883delinsT
4 mouse 269774 Aak1 AP2 associated kinase 1 XM_017321597.1 49% 50.8% (many diffs)
5 mouse 269774 Aak1 AP2 associated kinase 1 NM_177762.6 48.9% 50.7% (many diffs)
6 mouse 269774 Aak1 AP2 associated kinase 1 XM_006506192.1 44.8% 46.5% (many diffs)
7 mouse 269774 Aak1 AP2 associated kinase 1 NM_001040106.2 44.8% 46.5% (many diffs)
8 mouse 269774 Aak1 AP2 associated kinase 1 XM_006506191.1 44.5% 46.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1491
ORF length:
1425
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa gaagtttttc gactcccggc gagagcaggg cggctctggc ctgggctccg
121gctccagcgg aggagggggc agcacctcgg gcctgggcag tggctacatc ggaagagtct
181tcggcatcgg gcgacagcag gtcacagtgg acgaggtgtt ggcggaaggt ggatttgcta
241ttgtatttct ggtgaggaca agcaatggga tgaaatgtgc cttgaaacgc atgtttgtca
301acaatgagca tgatctccag gtgtgcaaga gagaaatcca gataatgagg gatctttcag
361ggcacaagaa tattgtgggt tacattgatt ctagtatcaa caacgtgagt agcggtgatg
421tatgggaagt gctcattctg atggactttt gtagaggtgg ccaggtggta aacctgatga
481accagcgcct gcaaacaggc tttacagaga atgaagtgct ccagatattt tgtgatacct
541gtgaagctgt tgcccgcctg catcagtgca aaactcctat tatccaccgg gacctgaagg
601ttgaaaacat cctcttgcat gaccgaggcc actatgtcct gtgtgacttt ggaagcgcca
661ccaacaaatt ccagaatcca caaactgagg gagtcaatgc agtagaagat gagattaaga
721aatacacaac gctgtcctat cgagcaccag aaatggtcaa cctgtacagt ggcaaaatca
781tcactacgaa ggcagacatt tgggctcttg gatgtttgtt gtataaatta tgctacttca
841ctttgccatt tggggaaagt caggtggcaa tttgtgatgg aaacttcaca attcctgata
901attctcgata ttctcaagac atgcactgcc taattaggta tatgttggaa ccagaccctg
961acaaaaggcc ggatatttac caggtgtcct acttctcatt taagctactc aagaaagagt
1021gcccaattcc aaatgtacag aactctccca ttcctgcaaa gcttcctgaa ccagtgaaag
1081ccagtgaggc agctgcaaaa aagacccagc caaaggccag actgacagat cccattccca
1141ccacagagac ttcaattgca ccccgccaga ggcctaaagc tgggcagact cagccgaacc
1201caggaatcct tcccatccag ccagcgctga caccccggaa gagggccact gttcagcccc
1261cacctcaggc tgcaggatcc agcaatcagc ctggcctttt agccagtgtt ccccaaccaa
1321aaccccaagc cccacccagc cagcctctgc cgcaaactca ggccaagcag ccacaggctc
1381ctcccactcc acagcagacg ccttctactc aggcccaggg tctgcccgct caggcccagg
1441ccacacccca gcaccagcag catacaataa aacttagtat gaaacttttn nccaactttc
1501ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
1561taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)