Construct: ORF ccsbBroad304_15001
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014881.1_s304c1, BRDN0000401916
- Derived from:
- ccsbBroadEn_15001
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB3GAP1 (22930)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15001
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | NM_012233.3 | 99.8% | 99.6% | (many diffs) |
| 2 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | NM_001172435.2 | 99.1% | 98.9% | (many diffs) |
| 3 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | XM_011510825.3 | 99.1% | 98.3% | (many diffs) |
| 4 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | XM_011510823.3 | 95% | 94.6% | (many diffs) |
| 5 | human | 22930 | RAB3GAP1 | RAB3 GTPase activating prot... | XR_001738674.2 | 80.6% | (many diffs) | |
| 6 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | NM_178690.4 | 87% | 92.6% | (many diffs) |
| 7 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XM_006529405.2 | 86.3% | 92% | (many diffs) |
| 8 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XR_001784075.1 | 63.9% | (many diffs) | |
| 9 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XR_001784076.1 | 63.5% | (many diffs) | |
| 10 | mouse | 226407 | Rab3gap1 | RAB3 GTPase activating prot... | XM_006529407.3 | 52% | 55.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3012
- ORF length:
- 2943
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgccgac agtgagcccg aatccgaggt atttgagatc acggacttca 121 ccactgcctc ggaatgggaa aggtttattt ccaaagttga agaagtcttg aatgactgga 181 aactgattgg aaactctttg ggaaagccac tcgaaaaggg tatatttact tctggcacat 241 gggaagagaa atcagatgaa atttcctttg ctgacttcaa gttctcagtc actcatcatt 301 atcttgtaca agagtccact gataaagaag gaaaggatga gttattagag gatgttgttc 361 cacaatctat gcaagatttg ctgggtatga ataatgactt tcctccaaga gcacattgcc 421 tggtaagatg gtatgggcta cgtgagttcg tggtgattgc ccctgctgca cacagtgacg 481 ctgttctcag cgaatctaag tgcaaccttc ttctgagttc tgtttctatt gccttgggaa 541 acactggctg tcaggtgcca ctctttgtgc aaattcacca caaatggcga agaatgtatg 601 taggagaatg tcaaggtcct ggtgtacgaa ctgatttcga aatggttcat cttagaaaag 661 tgccaaatca gtacactcac ttatcaggtc tgctggatat cttcaaatca aagattggat 721 gtcctttaac tccattgcct ccagttagta ttgctattcg atttacctat gtacttcaag 781 attggcagca gtatttttgg cctcagcaac ctccagacat agatgccctt gtaggaggag 841 aagttggagg cttggagttt ggcaagttac catttggtgc ctgcgaagat cctattagtg 901 aactccattt agctactaca tggcctcatc tgaccgaagg gatcattgtg gataatgatg 961 tttattctga tttggatcct attcaagctc cacattggtc tgttagagtt cgaaaagctg 1021 agaatcctca gtgtttgcta ggtgattttg tcactgaatt ttttaaaatt tgccgtcgaa 1081 aggagtcaac tgatgagatt cttggacgat ctgcatttga ggaagaaggc aaagaaactg 1141 ctgatataac tcatgctttg tcaaaattga cagagccggc atcagttcca attcataaat 1201 tatcagtttc aaatatggta cacactgcaa agaagaaaat ccgaaaacac agaggtgtag 1261 aggagtcacc gctaaataat gatgttctta atactattct cctgttctta ttccctgatg 1321 ctgtttctga gaaaccatta gatggaacta cttcaacaga taataataat cctccatcag 1381 agagtgaaga ctataatctc tacaatcagt tcaagtctgc accatctgac agtttaacat 1441 acaaactggc tttgtgtctc tgtatgatca atttttacca tggagggttg aaaggagtgg 1501 cacacctctg gcaggaattt gttcttgaaa tgcgtttccg atgggaaaac aactttctga 1561 ttccaggatt agcaagtgga cccccagatc tgaggtgttg tttactgcat cagatacgac 1621 agatgttaaa ttgttgtatt gaaagaaaga aggcacgtga tgaggggaaa aagacaagtg 1681 cttcagatgt cactaatata tatccagggg atgctggaaa agcaggagac cagttggtgc 1741 cagataatct aaaagaaaca gataaggaaa agggagaggt aggaaaatct tgggattcct 1801 ggagtgacag cgaagaagaa ttttttgaat gcctaagtga tactgaagaa cttaaaggaa 1861 atggacaaga gagtggcaag aaaggaggac ctaaggagat ggcaaattta aggccggaag 1921 gacggctcta tcagcatggg aaacttacac tgctgcataa tggagaacct ctctacattc 1981 cagtaaccca ggaaccagca cctatgacag aagatctgct agaagagcag tctgaagttt 2041 tagctaaatt aggtacatcg gcagaggggg ctcaccttcg agcacgcatg cagagtgcct 2101 gtctgctctc agatatggag tcttttaagg cagctaatcc aggttgctcc ctggaagatt 2161 ttgtgaggtg gtattcaccc cgggattata ttgaagagga ggtgattgat gaaaagggca 2221 atgtggtgct gaaaggagaa ctgagtgccc ggatgaagat tccaagcaat atgtgggtag 2281 aagcctggga aacagctaag ccaattcctg ctagaaggca aaggagactc tttgatgata 2341 cacgggaagc agaaaaggtg ctgcactatc tggcaatcca gaaacctgca gaccttgctc 2401 ggcacctgtt accttgtgtg attcatgcag ctgtactcaa ggtaaaggaa gaagaaagtc 2461 tcgaaaacat ttcttcagtt aagaagatca taaagcagat aatatcccat tccagtaaag 2521 ttttgcactt ccccaatcca gaagacaaga aattggaaga aatcattcac cagattacta 2581 atgtggaagc tctcattgcc agagctcggt cactaaaagc caagtttgga actgagaaat 2641 gtgaacagga ggaggaaaag gaagatcttg aaaggtttgt gagttgcctg ctggagcagc 2701 ctgaagtgtt agtcaccggt gcaggaagag gacatgctgg caggatcatt cacaagctgt 2761 ttgtgaatgc ccagagggct gcagctatga ctccaccaga ggaggaattg aagagaatgg 2821 gctccccaga ggaaagaagg cagaactccg tgtcagactt cccaccccct gctggccggg 2881 aattcatttt gcgcaccact gtgccgcgcc ctgctcccta ctccaaagct ctgcctcagc 2941 ggatgtacag tgttctcacc aaagaggact ttagacttgc aggtgccttt gcatcagata 3001 cgtccttctt cttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 3061 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 3121 caatcaacct ctggatta