Construct: ORF ccsbBroad304_15006

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007701.1_s304c1, BRDN0000409648
Derived from:
ccsbBroadEn_15006
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
NPIPB3 (23117)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15006
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 440353 NPIPB12 nuclear pore complex intera... NM_001355401.1 72.3% 28.7% (many diffs)
2 human 440345 NPIPB4 nuclear pore complex intera... XM_024450289.1 72.2% 28.9% (many diffs)
3 human 23117 NPIPB3 nuclear pore complex intera... NM_130464.3 59.3% 23.6% (many diffs)
4 human 100132247 NPIPB5 nuclear pore complex intera... NM_001135865.1 59.3% 23.6% (many diffs)
5 human 613037 NPIPB13 nuclear pore complex intera... NM_001321892.1 59% 23.4% (many diffs)
6 human 440345 NPIPB4 nuclear pore complex intera... XM_017023247.1 58.9% 23.5% (many diffs)
7 human 440345 NPIPB4 nuclear pore complex intera... XM_024450285.1 58.8% 23.5% (many diffs)
8 human 728888 NPIPB11 nuclear pore complex intera... NM_001310137.2 58.3% 23.5% (many diffs)
9 human 728888 NPIPB11 nuclear pore complex intera... XM_024450420.1 56.8% 22.9% (many diffs)
10 human 440345 NPIPB4 nuclear pore complex intera... NM_001310148.1 54.9% 22% (many diffs)
11 human 440345 NPIPB4 nuclear pore complex intera... XM_017023244.2 54.9% 22% (many diffs)
12 human 728734 NPIPB8 nuclear pore complex intera... NM_001310136.1 54% 54.6% (many diffs)
13 human 728734 NPIPB8 nuclear pore complex intera... XM_017023624.1 54% 54.6% (many diffs)
14 human 728734 NPIPB8 nuclear pore complex intera... XM_024450415.1 54% 54.6% (many diffs)
15 human 100507607 NPIPB9 nuclear pore complex intera... NM_001287250.1 53.8% 54.1% (many diffs)
16 human 100507607 NPIPB9 nuclear pore complex intera... XM_003118719.8 53.8% 54.1% (many diffs)
17 human 440345 NPIPB4 nuclear pore complex intera... XM_024450283.1 53.7% 21.5% (many diffs)
18 human 440345 NPIPB4 nuclear pore complex intera... XM_024450284.1 53% 18.9% (many diffs)
19 human 728741 NPIPB6 nuclear pore complex intera... NM_001282524.1 52.6% 55% (many diffs)
20 human 728741 NPIPB6 nuclear pore complex intera... XM_011545929.2 52.6% 55% (many diffs)
21 human 440348 NPIPB15 nuclear pore complex intera... XM_011523496.2 51.8% 42.6% (many diffs)
22 human 100507607 NPIPB9 nuclear pore complex intera... XM_011545693.3 51.7% 50.7% (many diffs)
23 human 440348 NPIPB15 nuclear pore complex intera... NM_001306094.1 51.6% 43.5% (many diffs)
24 human 440348 NPIPB15 nuclear pore complex intera... XM_011523498.3 51.6% 43.5% (many diffs)
25 human 440348 NPIPB15 nuclear pore complex intera... XM_017023248.1 51.6% 43.5% (many diffs)
26 human 100507607 NPIPB9 nuclear pore complex intera... NM_001287251.1 50.6% 45.8% (many diffs)
27 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022824.2 50.6% 45.8% (many diffs)
28 human 728734 NPIPB8 nuclear pore complex intera... XM_017023625.1 50.6% 45.8% (many diffs)
29 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022825.2 50.5% 46.6% (many diffs)
30 human 728734 NPIPB8 nuclear pore complex intera... XM_017023626.1 50.5% 46.6% (many diffs)
31 human 728734 NPIPB8 nuclear pore complex intera... XM_024450416.1 50.5% 46.6% (many diffs)
32 human 728734 NPIPB8 nuclear pore complex intera... XM_017023622.1 50.4% 49% (many diffs)
33 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022817.2 50.3% 48.6% (many diffs)
34 human 100507607 NPIPB9 nuclear pore complex intera... XM_011545690.3 50% 48.2% (many diffs)
35 human 728741 NPIPB6 nuclear pore complex intera... XM_017023628.1 49.3% 46.3% (many diffs)
36 human 728741 NPIPB6 nuclear pore complex intera... XM_005255741.4 49.3% 47.1% (many diffs)
37 human 728741 NPIPB6 nuclear pore complex intera... XM_017023630.1 49.3% 47.1% (many diffs)
38 human 728741 NPIPB6 nuclear pore complex intera... XM_024450419.1 49.3% 47.1% (many diffs)
39 human 728734 NPIPB8 nuclear pore complex intera... XM_024450417.1 49% 44.9% (many diffs)
40 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022814.2 48.4% 45.8% (many diffs)
41 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022815.2 48.4% 45.8% (many diffs)
42 human 440348 NPIPB15 nuclear pore complex intera... XM_024450290.1 48.1% 39.3% (many diffs)
43 human 728734 NPIPB8 nuclear pore complex intera... XM_017023623.1 47.9% 51.3% (many diffs)
44 human 728741 NPIPB6 nuclear pore complex intera... XM_011545931.2 47.8% 45.4% (many diffs)
45 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022818.2 47.4% 41% (many diffs)
46 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022819.2 47.3% 41.1% (many diffs)
47 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022820.2 47.2% 41.9% (many diffs)
48 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022821.2 47.2% 41.9% (many diffs)
49 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022822.2 47.2% 41.9% (many diffs)
50 human 728734 NPIPB8 nuclear pore complex intera... XM_024450418.1 46.9% 42.2% (many diffs)
51 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022816.2 46% 47.8% (many diffs)
52 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022823.2 45.8% 40.4% (many diffs)
53 human 728741 NPIPB6 nuclear pore complex intera... XM_017023631.2 45.6% 42.7% (many diffs)
54 human 642799 NPIPA2 nuclear pore complex intera... XM_024450385.1 43.6% 55.1% (many diffs)
55 human 642799 NPIPA2 nuclear pore complex intera... XM_024450386.1 43.6% 55.1% (many diffs)
56 human 642778 NPIPA3 nuclear pore complex intera... XM_017023554.1 43.6% 55.1% (many diffs)
57 human 642778 NPIPA3 nuclear pore complex intera... XM_024450379.1 43.6% 55.1% (many diffs)
58 human 100288332 NPIPA5 nuclear pore complex intera... XM_024450132.1 43.5% 54.6% (many diffs)
59 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022833.2 43% 42.2% (many diffs)
60 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030211.2 42.8% 41.7% (many diffs)
61 human 101059938 NPIPA7 nuclear pore complex intera... XM_011522339.3 42.8% 44% (many diffs)
62 human 101059938 NPIPA7 nuclear pore complex intera... XM_005255735.5 42.8% 41.7% (many diffs)
63 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452519.1 42.4% 42.6% (many diffs)
64 human 101059938 NPIPA7 nuclear pore complex intera... NM_001282507.1 42.3% 42.6% (many diffs)
65 human 101059953 NPIPA8 nuclear pore complex intera... NM_001282511.2 42.3% 42.6% (many diffs)
66 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022834.1 42.3% 42.6% (many diffs)
67 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022835.1 42.3% 42.6% (many diffs)
68 human 642799 NPIPA2 nuclear pore complex intera... XM_024450381.1 41.9% 51.3% (many diffs)
69 human 642799 NPIPA2 nuclear pore complex intera... XM_024450382.1 41.9% 51.3% (many diffs)
70 human 642799 NPIPA2 nuclear pore complex intera... XM_024450383.1 41.9% 51.3% (many diffs)
71 human 642778 NPIPA3 nuclear pore complex intera... XM_011522576.2 41.9% 51.3% (many diffs)
72 human 642778 NPIPA3 nuclear pore complex intera... XM_011522577.2 41.9% 51.3% (many diffs)
73 human 642778 NPIPA3 nuclear pore complex intera... XM_017023553.1 41.9% 51.3% (many diffs)
74 human 642799 NPIPA2 nuclear pore complex intera... XM_024450384.1 40.5% 43.8% (many diffs)
75 human 440345 NPIPB4 nuclear pore complex intera... XM_024450286.1 40.5% 7.2% (many diffs)
76 human 642799 NPIPA2 nuclear pore complex intera... XM_024450388.1 40.5% 46.4% (many diffs)
77 human 642778 NPIPA3 nuclear pore complex intera... XM_011522578.2 40.5% 43.8% (many diffs)
78 human 642778 NPIPA3 nuclear pore complex intera... XM_011522580.2 40.5% 46.4% (many diffs)
79 human 642799 NPIPA2 nuclear pore complex intera... NM_001277324.1 40.1% 44.9% (many diffs)
80 human 440345 NPIPB4 nuclear pore complex intera... XM_024450287.1 40% 7.1% (many diffs)
81 human 440345 NPIPB4 nuclear pore complex intera... XM_024450288.1 40% 7.1% (many diffs)
82 human 642778 NPIPA3 nuclear pore complex intera... NM_001277323.1 40% 44.9% (many diffs)
83 human 9284 NPIPA1 nuclear pore complex intera... NM_006985.4 40% 44.7% (many diffs)
84 human 100288332 NPIPA5 nuclear pore complex intera... NM_001277325.2 40% 44.4% (many diffs)
85 human 729978 NPIPB2 nuclear pore complex intera... XM_024450424.1 38.3% 38.1% (many diffs)
86 human 729978 NPIPB2 nuclear pore complex intera... XM_024450425.1 38.3% 38.1% (many diffs)
87 human 729978 NPIPB2 nuclear pore complex intera... XM_024450426.1 38.3% 38.1% (many diffs)
88 human 728888 NPIPB11 nuclear pore complex intera... XM_024450422.1 33.9% 63.6% (many diffs)
89 human 728888 NPIPB11 nuclear pore complex intera... XM_024450421.1 33.5% 69.5% (many diffs)
90 human 728741 NPIPB6 nuclear pore complex intera... XM_005255742.4 32.3% 62.9% (many diffs)
91 human 728741 NPIPB6 nuclear pore complex intera... XM_005255743.3 32% 21.8% (many diffs)
92 human 440348 NPIPB15 nuclear pore complex intera... XM_011523501.2 31.7% 18.5% (many diffs)
93 human 729978 NPIPB2 nuclear pore complex intera... NM_001355514.1 31.2% 28.5% (many diffs)
94 human 729978 NPIPB2 nuclear pore complex intera... XM_024450428.1 31.2% 28.5% (many diffs)
95 human 729978 NPIPB2 nuclear pore complex intera... XM_024450431.1 31.2% 28.5% (many diffs)
96 human 100507607 NPIPB9 nuclear pore complex intera... XM_017022827.2 31.1% 19.4% (many diffs)
97 human 729978 NPIPB2 nuclear pore complex intera... XM_024450427.1 31% 54.3% (many diffs)
98 human 729978 NPIPB2 nuclear pore complex intera... XM_024450429.1 27.8% 22.7% (many diffs)
99 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022836.2 27.7% 23.7% (many diffs)
100 human 101059938 NPIPA7 nuclear pore complex intera... XM_017022840.2 27.7% 23.7% (many diffs)
101 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030214.2 27.7% 23.7% (many diffs)
102 human 102724993 LOC102724993 nuclear pore complex-intera... XR_001756353.1 26.4% (many diffs)
103 human 642799 NPIPA2 nuclear pore complex intera... XM_006720917.4 26.2% 45.6% (many diffs)
104 human 100288332 NPIPA5 nuclear pore complex intera... XM_011522336.3 26.2% 45.6% (many diffs)
105 human 642778 NPIPA3 nuclear pore complex intera... XM_011522581.1 26.2% 45.6% (many diffs)
106 human 642778 NPIPA3 nuclear pore complex intera... XM_011522583.3 26.2% 45.6% (many diffs)
107 human 642799 NPIPA2 nuclear pore complex intera... XM_011522593.1 26.2% 45.6% (many diffs)
108 human 642799 NPIPA2 nuclear pore complex intera... XM_011522594.3 26.2% 45.6% (many diffs)
109 human 642778 NPIPA3 nuclear pore complex intera... XM_011522584.2 25.6% 46.6% (many diffs)
110 human 642799 NPIPA2 nuclear pore complex intera... XM_011522595.2 25.6% 46.6% (many diffs)
111 human 729978 NPIPB2 nuclear pore complex intera... XM_024450430.1 25.4% 51.5% (many diffs)
112 human 102724993 LOC102724993 nuclear pore complex-intera... XM_017030213.2 24.6% 37% (many diffs)
113 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452521.1 24% 38% (many diffs)
114 human 102724993 LOC102724993 nuclear pore complex-intera... XM_024452522.1 23.8% 38% (many diffs)
115 human 100288332 NPIPA5 nuclear pore complex intera... NM_001351200.1 23.8% 37.7% (many diffs)
116 human 642778 NPIPA3 nuclear pore complex intera... XM_005255485.4 23.8% 37.7% (many diffs)
117 human 642799 NPIPA2 nuclear pore complex intera... XM_005255492.4 23.8% 37.7% (many diffs)
118 human 101059938 NPIPA7 nuclear pore complex intera... XM_024450138.1 23.7% 38% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
903
ORF length:
837
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gaagctctct attgtcctga ccccacagtt cctgtcccat gaccagggcc
121agctctccaa ggagctgcag cagcatgtaa agtcagtgac atgcccatgc gagtacctga
181ggaaggttat caatactctg gctgaccatc atcatcgtgg gactgacttt ggtggaagtc
241cttggttaca tgtcattatt gcgtttccga caagttataa agttgtcatt accctctgga
301tagtttacct ttgggtgtct ctcctgaaga ctatcttctg gtctcgaaat ggacatgatg
361gatccacgga tgtacagcag agagcctgga ggtccaaccg ccgtagacag gaagggctga
421ggtccatttg tatgcacaca aagaaaagag tttcttcctt tcgaggaaat aaaattgtcc
481tgaaagacgt cattactcta cggagacatg tggaaacaaa agttagagct aaaatccgta
541agaggaaggt gacaacgaaa atcaaccatc atgacaaaat caatggaaag aggaagaccg
601ccagaaaaca gaaaatgttt caacgtgcgc aagagttgcg gcggcgagca gaggactacc
661acaaatgcaa aatcccccct tctgcaagaa aggctctttg caactgggtc agaatggcgg
721cagcggagca tcgtcattct tcaggattgc cctactggcc ctacctcaca gctgaaactt
781taaaaaacag gatgggccac cagccacctc ctccaactca acaacattct ataactgata
841actccctgag cctcaagaca cctcccttag tgtctgctca ctccccttcc accctcagcg
901gatgataatc tcaagacacc tcccgagtgt gtgctcactc cccttccacc ctcagcggat
961gataatctca agacacctcc cgagtgtgtg ctcactcccc ttccaccctc agcggatgat
1021aatctcaaga cacctcctga gtgtctgctc actccccttc caccctcagc ggatgataat
1081ctcaagacac ctcccgagtg tctactcact ccccttccac cctcagctct accctcagct
1141ccaccctcag cggatgataa tctcaagaca cgtgccgagt gtctgctcca tccccttcca
1201ccctcagcag atgataatct caagacactt tccgagcgtc agctcactcc ccttccaccc
1261tcagctccac cctcagcaga tgataatatc aagacacctg ccgagcgtct gcgggggccg
1321cttccaccct cagcggatga taatctcaag acaccttccg agcgtcagct cactcccctt
1381ccaccctcag ctccaccctc agcagatgat aatatcaaga cacctgccga gcgtctgcgg
1441gggccgcttc caccctcagc ggatgataat ctcaagacac cttccgagcg tcagctcact
1501ccccttccac cctcagctcc accctcagca gatgataata tcaagacacc tgccgagcgt
1561ctgcgggggc cgcttccacc ctcagcggat gataatctca agacaccttc cgagcgtcag
1621ctcactcccc ttccaccctc agctccaccc tcagcagatg ataatatcaa gacacctgcc
1681gagcgtctgc gggggccgct tccaccctca gccgatgaga gcctcaagac acctccctta
1741gctactcagg aggctgaggc agaaaaacca cgcaaaccca agaggcagag ggcggctgag
1801atggaaccac ctcccgaacc caagaggcgg aggttgagcg acgtggaacc gtcacgcaaa
1861cccaagaggc ggagggccgc tgacgtggaa ccatcatcac ccgaacccaa gaggcggagg
1921gtcggtgatg tggaaccgtc acgcaaaccc aagaggcgga gggccgctga cgtggaacca
1981tcatcacccg aacccaagag gcggagggtc ggtgacgtgg aaccgtcacg caaacccaag
2041aggcggaggg ccgctgacgt ggaaccatca cctcccgaac ccaagaggcg gaggttgagc
2101tgcccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc
2161gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc
2221tggatta

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