Construct: ORF ccsbBroad304_15021
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011828.2_s304c1, BRDN0000459560
- Derived from:
- ccsbBroadEn_15021
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3BP4 (23677)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15021
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23677 | SH3BP4 | SH3 domain binding protein 4 | NM_001371302.1 | 100% | 100% | |
| 2 | human | 23677 | SH3BP4 | SH3 domain binding protein 4 | NM_001371303.1 | 100% | 100% | |
| 3 | human | 23677 | SH3BP4 | SH3 domain binding protein 4 | NM_001371304.1 | 100% | 100% | |
| 4 | human | 23677 | SH3BP4 | SH3 domain binding protein 4 | NM_001371305.1 | 100% | 100% | |
| 5 | human | 23677 | SH3BP4 | SH3 domain binding protein 4 | NM_001371306.1 | 100% | 100% | |
| 6 | human | 23677 | SH3BP4 | SH3 domain binding protein 4 | NM_014521.3 | 100% | 100% | |
| 7 | human | 23677 | SH3BP4 | SH3 domain binding protein 4 | XM_011510893.1 | 100% | 100% | |
| 8 | mouse | 98402 | Sh3bp4 | SH3-domain binding protein 4 | NM_133816.2 | 85.7% | 91.5% | (many diffs) |
| 9 | mouse | 98402 | Sh3bp4 | SH3-domain binding protein 4 | XM_006529975.3 | 85.7% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2955
- ORF length:
- 2889
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctcagcgg atccgagcgg ccaactccaa tggcctccct cgctgcaagt 121 cagaggggac cctgattgac ctgagcgaag ggttttcaga gacgagcttt aatgacatca 181 aagtgccttc tcccagtgcc ttgctcgtag acaaccccac acctttcgga aatgcaaagg 241 aagtgattgc gatcaaggac tattgcccca ccaacttcac cacactgaag ttctccaagg 301 gcgaccatct ctacgtcttg gacacatctg gcggtgagtg gtggtacgca cacaacacca 361 ccgaaatggg ctacatcccc tcctcctatg tgcagccctt gaactaccgg aactcaacac 421 tgagtgacag cggtatgatt gataatcttc cagacagccc agacgaggta gccaaggagc 481 tggagctgct cgggggatgg acagatgaca aaaaagtacc aggcagaatg tacagtaata 541 accctttctg gaatggggtc cagaccaatc catttctgaa tgggaacgtg cccgtcatgc 601 ccagcctgga tgagctgaat cccaaaagta ctgtggattt gctccttttt gacgcaggta 661 catcctcctt caccgaatcc agctcagcca ccacgaatag cactggcaac atcttcgatg 721 agcttccagt cacaaacgga ctccacgcag agccgccggt caggcgggac aaccccttct 781 tcagaagcaa gcgctcctac agtctctcgg aactctccgt cctccaagcc aagtccgatg 841 ctcccacatc gtcgagtttc ttcaccggct tgaaatcacc tgcccccgag caatttcaga 901 gccgggagga ttttcgaact gcctggctaa accacaggaa gctggcccgg tcttgccacg 961 acctggactt gcttggccaa agccctggtt ggggccagac ccaagccgtg gagacaaaca 1021 tcgtgtgcaa gctggatagc tccgggggtg ctgtccagct tcctgacacc agcatcagca 1081 tccacgtgcc cgagggccac gtcgcccctg gggagaccca gcagatctcc atgaaagccc 1141 tgctggaccc cccgctggag ctcaacagtg acaggtcctg cagcatcagc cctgtgctgg 1201 aggtcaagct gagcaacctg gaggtgaaaa cctctatcat cttggagatg aaagtgtcag 1261 ccgagataaa aaatgacctt tttagcaaaa gcacagtggg cctccagtgc ctgaggagcg 1321 actcgaagga agggccatat gtctccgtcc cgctcaactg cagctgtggg gacacggtcc 1381 aggcacagct gcacaacctg gagccctgta tgtacgtggc tgtcgtggcc catggcccaa 1441 gcatcctcta cccttccacc gtgtgggact tcatcaataa aaaagtcaca gtgggtctct 1501 acggccctaa acacatccac ccatccttca agacggtagt gaccattttt gggcatgact 1561 gtgccccaaa gacgctcctg gtcagcgagg tcacacgcca ggcacccaac cctgccccgg 1621 tggccctgca gctgtggggg aagcaccagt tcgttttgtc caggccccag gatctcaagg 1681 tctgtatgtt ttccaatatg acgaattacg aggtcaaagc cagcgagcag gccaaagtgg 1741 tgcgaggatt ccagctgaag ctgggcaagg tgagccgcct gatcttcccc atcacctccc 1801 agaaccccaa cgagctctct gacttcacgc tgcgggttca ggtgaaggac gaccaggagg 1861 ccatcctcac ccagttttgt gtccagactc ctcagccacc ccctaaaagt gccatcaagc 1921 cttccgggca aaggaggttt ctcaagaaga acgaagtcgg gaaaatcatc ctgtccccgt 1981 ttgccaccac tacaaagtac ccgactttcc aggaccgccc ggtgtccagc ctcaagtttg 2041 gtaagttgct caagactgtg gtgcggcaga acaagaacca ctacctgctg gagtacaaga 2101 agggcgacgg gatcgccctg ctcagcgagg agcgggtcag gctccggggc cagctgtgga 2161 ccaaggagtg gtacatcggc tactaccagg gcagggtggg cctcgtgcac accaagaacg 2221 tgctggtggt cggcagggcc cggcccagcc tgtgctcggg ccccgagctg agcacctcgg 2281 tgctgctgga gcagatcctg cggccctgca aattcctcac gtacatctat gcctccgtga 2341 ggaccctgct catggagaac atcagcagct ggcgctcctt cgctgacgcc ctgggctacg 2401 tgaacctgcc gctcaccttt ttctgccggg cagagctgga tagtgagccc gagcgggtgg 2461 cgtccgtcct agaaaagctg aaggaggact gtaacaacac tgagaacaaa gaacggaagt 2521 ccttccagaa ggagcttgtg atggccctac tgaagatgga ctgccagggc ctggtggtca 2581 gactcatcca ggactttgtg ctcctgacca cggctgtaga ggtggcccag cgctggcggg 2641 agctggctga gaagctggcc aaggtctcca agcagcagat ggacgcctac gagtctcccc 2701 accgggacag gaacggggtt gtggacagcg aggccatgtg gaagcctgcg tatgacttct 2761 tactcacctg gagccatcag atcggggaca gctaccggga tgtcatccag gagctgcacc 2821 tgggcctgga caagatgaaa aaccccatca ccaagcgctg gaagcacctc actgggactc 2881 tgatcttggt gaactccctg gacgttctga gagcagccgc cttcagccct gcggaccagg 2941 acgacttcgt gatttgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 3001 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 3061 cgacaatcaa cctctggatt a