Transcript: Human NM_001371304.1

Homo sapiens SH3 domain binding protein 4 (SH3BP4), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-07-10
Taxon:
Homo sapiens (human)
Gene:
SH3BP4 (23677)
Length:
5110
CDS:
321..3212

Additional Resources:

NCBI RefSeq record:
NM_001371304.1
NBCI Gene record:
SH3BP4 (23677)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147097 CATCCACCCATCCTTCAAGA pXPR_003 CGG 1465 51% 4 0.6685 SH3BP4 SH3BP4 78090
2 BRDN0001149502 GTGAAGAAACTCGACGATGT pXPR_003 GGG 783 27% 4 0.4134 SH3BP4 SH3BP4 78088
3 BRDN0001149529 TGAAGTCAGAGAGCTCGTTG pXPR_003 GGG 1745 60% 4 0.3554 SH3BP4 SH3BP4 78087
4 BRDN0001487091 GCTGAATCCCAAAAGTACTG pXPR_003 TGG 565 20% 4 0.091 SH3BP4 SH3BP4 78089
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001371304.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127812 GAGTTTCTTCACCGGCTTGAA pLKO.1 1109 CDS 100% 4.950 6.930 N SH3BP4 n/a
2 TRCN0000292184 GAGTTTCTTCACCGGCTTGAA pLKO_005 1109 CDS 100% 4.950 6.930 N SH3BP4 n/a
3 TRCN0000130594 CCACACCTTTCGGAAATGCAA pLKO.1 472 CDS 100% 3.000 4.200 N SH3BP4 n/a
4 TRCN0000292185 CCACACCTTTCGGAAATGCAA pLKO_005 472 CDS 100% 3.000 4.200 N SH3BP4 n/a
5 TRCN0000381961 CTTTCACTCCCTGCGTGTAAG pLKO_005 3710 3UTR 100% 10.800 8.640 N SH3BP4 n/a
6 TRCN0000379832 ATGAGAAAGGTGGTATCTAAT pLKO_005 3493 3UTR 100% 13.200 9.240 N SH3BP4 n/a
7 TRCN0000146508 CGTGTGGGACTTCATCAATAA pLKO.1 1715 CDS 100% 13.200 9.240 N SH3BP4 n/a
8 TRCN0000146356 CACACCTTTCGGAAATGCAAA pLKO.1 473 CDS 100% 4.950 3.465 N SH3BP4 n/a
9 TRCN0000130786 GTCAGACTCATCCAGGACTTT pLKO.1 2832 CDS 100% 4.950 3.465 N SH3BP4 n/a
10 TRCN0000292183 GTCAGACTCATCCAGGACTTT pLKO_005 2832 CDS 100% 4.950 3.465 N SH3BP4 n/a
11 TRCN0000130838 GATGAAAGTGTCAGCCGAGAT pLKO.1 1502 CDS 100% 4.050 2.835 N SH3BP4 n/a
12 TRCN0000292186 GATGAAAGTGTCAGCCGAGAT pLKO_005 1502 CDS 100% 4.050 2.835 N SH3BP4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001371304.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02830 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02830 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_15021 pDONR223 0% 100% 100% None n/a
4 ccsbBroad304_15021 pLX_304 0% 100% 100% V5 n/a
5 TRCN0000468043 CATACAATATCCGGCGAACTCTTT pLX_317 13.3% 100% 100% V5 n/a
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