Construct: ORF ccsbBroad304_15039
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001932.2_s304c1, BRDN0000459576
- Derived from:
- ccsbBroadEn_15039
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPK1 (27010)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15039
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350879.1 | 99.7% | 99.5% | 578_579delTGinsGT |
| 2 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_022445.4 | 99.7% | 99.5% | 578_579delTGinsGT |
| 3 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_005249970.1 | 99.7% | 99.5% | 578_579delTGinsGT |
| 4 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516031.1 | 90% | 89.9% | 256_333del;656_657delTGinsGT |
| 5 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516032.2 | 90% | 89.9% | 256_333del;656_657delTGinsGT |
| 6 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516033.2 | 90% | 89.9% | 256_333del;656_657delTGinsGT |
| 7 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516034.2 | 90% | 89.9% | 256_333del;656_657delTGinsGT |
| 8 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350881.1 | 89.7% | 86% | (many diffs) |
| 9 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516035.3 | 81.3% | 77.6% | (many diffs) |
| 10 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011969.1 | 81% | 73.1% | (many diffs) |
| 11 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011972.1 | 80% | 76.5% | (many diffs) |
| 12 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001042482.1 | 79.5% | 79.4% | 354_355ins147;431_432delTGinsGT |
| 13 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350880.1 | 79.5% | 79.4% | 354_355ins147;431_432delTGinsGT |
| 14 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516040.2 | 79.3% | 76.2% | (many diffs) |
| 15 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516043.1 | 71.8% | 71.7% | 256_333del;432_433ins147;509_510delTGinsGT |
| 16 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350882.1 | 69.5% | 76.4% | 0_1ins136;48_168del;563_564delTGinsGT |
| 17 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350883.1 | 69.5% | 76.4% | 0_1ins136;48_168del;563_564delTGinsGT |
| 18 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350884.2 | 69.5% | 76.4% | 0_1ins136;48_168del;563_564delTGinsGT |
| 19 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516039.2 | 68.3% | 64% | (many diffs) |
| 20 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011974.1 | 68.3% | 64% | (many diffs) |
| 21 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011975.1 | 68.3% | 64% | (many diffs) |
| 22 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011980.2 | 66.1% | 64.6% | (many diffs) |
| 23 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011981.2 | 66.1% | 64.6% | (many diffs) |
| 24 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516037.2 | 63.6% | 69.3% | (many diffs) |
| 25 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011970.1 | 63.6% | 69.3% | (many diffs) |
| 26 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011971.1 | 63.6% | 69.3% | (many diffs) |
| 27 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350885.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 28 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350886.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 29 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350887.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 30 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350889.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 31 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350893.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 32 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350894.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 33 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516047.2 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 34 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516048.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 35 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_017011982.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 36 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_024446717.1 | 56.1% | 55.9% | 0_1ins318;260_261delTGinsGT |
| 37 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_024446715.1 | 52.2% | 57.2% | (many diffs) |
| 38 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NM_001350895.1 | 51.4% | 51% | 0_1ins351;2T>A;227_228delTGinsGT |
| 39 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_024446716.1 | 51.4% | 51% | 0_1ins351;2T>A;227_228delTGinsGT |
| 40 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XM_011516046.1 | 44.4% | 43.8% | (many diffs) |
| 41 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | XR_001744630.1 | 35.5% | (many diffs) | |
| 42 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NR_146936.2 | 24.1% | (many diffs) | |
| 43 | human | 27010 | TPK1 | thiamin pyrophosphokinase 1 | NR_146934.1 | 23.6% | (many diffs) | |
| 44 | mouse | 29807 | Tpk1 | thiamine pyrophosphokinase | NM_013861.4 | 86.8% | 89.3% | (many diffs) |
| 45 | mouse | 29807 | Tpk1 | thiamine pyrophosphokinase | XM_006506219.3 | 78.9% | 81% | (many diffs) |
| 46 | mouse | 29807 | Tpk1 | thiamine pyrophosphokinase | NM_001311111.1 | 70% | 70.7% | (many diffs) |
| 47 | mouse | 29807 | Tpk1 | thiamine pyrophosphokinase | XM_006506218.3 | 67.2% | 66.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 795
- ORF length:
- 729
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gcatgccttt accccgttgg agcccctgct ttccactggg aatttgaagt 121 actgccttgt aattcttaat cagcctttgg acaactattt tcgtcatctt tggaacaaag 181 ctcttttaag agcctgtgcc gatggaggtg ccaaccgctt atatgatatc accgaaggag 241 agagagaaag ctttttgcct gaattcatca atggagactt tgattctatt aggcctgaag 301 tcagagaata ctatgctact aagggatgtg agctcatttc aactcctgat caagaccaca 361 ctgactttac taagtgcctt aaaatgctcc aaaagaagat agaagaaaaa gacttaaagg 421 ttgatgtgat cgtgacactg ggaggccttg ctgggcgttt tgaccagatt atggcatctg 481 tgaatacctt gttccaagcg actcacatca ctccttttcc aattataata atccaagagg 541 aatcgctgat ctacctgctc caaccaggaa agcacaggtt gcatgtagac actggaatgg 601 agggtgattg gtgtggcctt attcctgttg gacagccttg tagtcaggtt acaaccacag 661 gcctcaagtg gaacctcaca aatgatgtgc ttgcttttgg aacattggtc agtacttcca 721 atacctacga cgggtctggt gttgtgactg tggaaactga ccacccactc ctctggacca 781 tggccatcaa aagctaccca actttcttgt acaaagtggt tggtaagcct atccctaacc 841 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 901 cgacaatcaa cctctggatt a