Construct: ORF ccsbBroad304_15081

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018912.1_s304c1, BRDN0000459396
Derived from:
ccsbBroadEn_15081
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
TRPM7 (54822)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15081
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54822 TRPM7 transient receptor potentia... NM_001301212.2 96.6% 12.1% (many diffs)
2 human 54822 TRPM7 transient receptor potentia... NM_017672.6 96.6% 12.1% (many diffs)
3 human 54822 TRPM7 transient receptor potentia... XM_017022353.2 96.1% 12% (many diffs)
4 human 54822 TRPM7 transient receptor potentia... XM_017022352.1 96.1% 12% (many diffs)
5 human 54822 TRPM7 transient receptor potentia... XM_005254486.4 95.5% 12% (many diffs)
6 human 54822 TRPM7 transient receptor potentia... XM_017022351.1 95.1% 11.9% (many diffs)
7 human 54822 TRPM7 transient receptor potentia... XM_017022350.1 95.1% 11.9% (many diffs)
8 human 54822 TRPM7 transient receptor potentia... XM_017022354.1 92.5% 8.9% (many diffs)
9 human 54822 TRPM7 transient receptor potentia... XM_017022355.1 92.5% 8.9% (many diffs)
10 human 54822 TRPM7 transient receptor potentia... XR_001751327.1 81.4% (many diffs)
11 human 54822 TRPM7 transient receptor potentia... XR_002957654.1 76.7% (many diffs)
12 human 54822 TRPM7 transient receptor potentia... XR_001751325.1 75.6% (many diffs)
13 human 54822 TRPM7 transient receptor potentia... XR_001751328.2 75.3% (many diffs)
14 human 54822 TRPM7 transient receptor potentia... XR_001751326.2 75.3% (many diffs)
15 human 54822 TRPM7 transient receptor potentia... XR_002957653.1 61.8% (many diffs)
16 human 54822 TRPM7 transient receptor potentia... NR_149152.2 51.9% (many diffs)
17 human 54822 TRPM7 transient receptor potentia... NR_149153.2 51.2% (many diffs)
18 human 54822 TRPM7 transient receptor potentia... NR_149154.2 50.5% (many diffs)
19 mouse 58800 Trpm7 transient receptor potentia... NM_001164325.1 87.9% 12% (many diffs)
20 mouse 58800 Trpm7 transient receptor potentia... NM_021450.2 87.9% 12% (many diffs)
21 mouse 58800 Trpm7 transient receptor potentia... XM_017319154.1 73% 14.4% (many diffs)
22 mouse 58800 Trpm7 transient receptor potentia... XR_001783160.1 68.8% (many diffs)
23 mouse 58800 Trpm7 transient receptor potentia... XR_001783159.1 68.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
816
ORF length:
747
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcccagaaa tcctggatag aaagcacttt gaccaagagg gaatgtgtat
121atattatacc aagttccaag gaccctcaca gatgccttcc aggatgtcaa atttgtcagc
181aactcgtcag gtgtttttgt ggtcgcttgg tcaagcaaca tgcttgtttt actgcaagtc
241ttgccatgaa atactcagat gtgaaattgg gtgaccattt taatcaggca atagaagaat
301ggtctgtgga aaagcataca gaacagagcc caacggatgc ttatggagtc ataaattttc
361aagggggttc tcattcctac agagctaagt atgtgaggct atcatatgac accaaacctg
421aagtcattct gcaacttctg cttaaagaat ggcaaatgga gttacccaaa cttgttatct
481ctgtacatgg gggcatgcag aaatttgagc ttcacccacg aatcaagcag ttgcttggaa
541aaggtcttat taaagctgca gttacaactg gagcctggat tttaactgga ggagtaaaca
601caggtgtggc aaaacatgtt ggagatgccn nnaaagaaca tgcttcnaga tcatctcgaa
661nnntttgcac tatcggaata gctccatggg gagtgattga aaacagaaat gatcttgttg
721ggagagatgt ggttgctcct tatcaancnc ttatnnaacc ccctgagcaa attgaatgtt
781ttgaatnatc tgcattccca tttcatattg gtggatgatg gcactgttgg aaagtatggg
841gcggaagtca gactgagaag aagaacttga aaaaacttat taatcagcaa agaattcatg
901ctaggattgg ccagggtgtc cctgtggtgg cacttatatt tgagggtggg ccaaatgtta
961tcctcacagt tcttgaatac cttcaggaaa gcccccctgt tccagtagtt gtgtgtgaag
1021gaacaggcag agctgcagat ctgctagcgt atattcataa acaaacagaa gaaggaggga
1081atcttcctga tgcagcagag cccgatatta tttccactat caaaaaaaca tttaactttg
1141gccagaatga agcacttcat ttatttcaaa cactgatgga gtgcatgaaa agaaaggagc
1201ttatcactgt tttccatatt gggtcagatg aacatcaaga tatagatgta gcaatactta
1261ctgcactgct aaaagatctg aagaggaatg attatacgcc tgataaaatt atatttcctc
1321aggatgagcc ttcagatttg aatcttcagc ctggaaattc caccaaagaa tcagaatcaa
1381ctaattctgt tcgtctgatg ttattgccaa ctttcttgta caaagttggc attataagaa
1441agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
1501ttgccatccc agcctaaaac tggaagaact ttacaacact aaacaaggtc caactaatcc
1561aatgctgttt catcttgttc gagacgtcaa acagggaaat cttcctccag gatataagat
1621cactctgatt gatataggac ttgttattga atatctcatg ggaggaacct acagatgcac
1681ctatactagg aaacgttttc gattaatata taatagtctt ggtggaaata atcggaggtc
1741tggccgaaat acctccagca gcactcctca gttgcgaaag agtcatgaat cttttggcaa
1801tagggcagat aaaaaggaaa aaatgaggca taaccatttc attaagacag cacagcccta
1861ccgaccaaag attgatacag ttatggaaga aggaaagaag aaaagaacca aagatgaaat
1921tgtagacatt gatgatccag aaaccaagcg ctttccttat ccacttaatg aacttttaat
1981ttgggcttgc cttatgaaga ggcaggtcat ggcccgtttt ttatggcaac atggtgaaga
2041atcaatggct aaagcattag ttgcctgtaa gatctatcgt tcaatggcat atgaagcaaa
2101gcagagtgac ctggtagatg atacttcaga agaactaaaa cagtattcca atgattttgg
2161tcagttggcc gttgaattat tagaacagtc cttcagacaa gatgaaacca tggctatgaa
2221attgctcact tatgaactga agaactggag taattcaacc tgccttaagt tagcagtttc
2281ttcaagactt agaccttttg tagctcacac ctgtacacaa atgttgttat ctgatatgtg
2341gatgggaagg ctgaatatga ggaaaaattc ctggtacaag gtcatactaa gcattttagt
2401tccacctgcc atattgctgt tagagtataa aactaaggct gaaatgtccc atatcccaca
2461atctcaagat gctcatcaga tgacaatgga tgacagcgaa aacaactttc agaacataac
2521agaagagatc cccatggaag tgtttaaaga agtacggatt ttggatagta atgaaggaaa
2581gaatgagatg gagatacaaa tgaaatcaaa aaagcttcca attacgcgaa agttttatgc
2641cttttatcat gcaccaattg taaaattctg gtttaacacg ttggcatatt taggatttct
2701gatgctttat acatttgtgg ttcttgtaca aatggaacag ttaccttcag ttcaagaatg
2761gattgttatt gcttatattt ttacttatgc cattgagaaa gtccgtgaga tctttatgtc
2821tgaagctggg aaagtaaacc agaagattaa agtatggttt agtgattact tcaacatcag
2881tgatacaatt gccataattt ctttcttcat tggatttgga ctaagatttg gagcaaaatg
2941gaactttgca aatgcatatg ataatcatgt ttttgtggct ggaagattaa tttactgtct
3001taacataata ttttggtatg tgcgtttgct agattttcta gctgtaaatc aacaggcagg
3061accttatgta atgatgattg gaaaaatggt ggccaatatg ttctacattg tagtgattat
3121ggctcttgta ttacttagtt ttggtgttcc cagaaaggca atactttatc ctcatgaagc
3181accatcttgg actcttgcta aagatatagt ttttcaccca tactggatga tttttggtga
3241agtttatgca tacgaaattg atgtgtgtgc aaatgattct gttatccctc aaatctgtgg
3301tcctgggacg tggttgactc catttcttca agcagtctac ctctttgtac agtatatcat
3361tatggttaat cttcttattg catttttcaa caatgtgtat ttacaagtga aggcaatttc
3421caatattgta tggaagtacc agcgttatca ttttattatg gcttatcatg agaaaccagt
3481tctgcctcct ccacttatca ttcttagcca tatagtttct ctgttttgct gcatatgtaa
3541gagaagaaag aaagataaga cttccgatgg accaaaactt ttcttaacag aagaagatca
3601aaagaaactt catgattttg aagagcagtg tgttgaaatg tatttcaatg aaaaagatga
3661caaatttcat tctgggagtg aagagagaat tcgtgtcact tttgaaagag tggaacagat
3721gtgcattcag attaaagaag ttggagatcg tgtcaactac ataaaaagat cattacaatc
3781attagattct caaattggcc atttgcaaga tctttcagcc ctgacggtag atacattaaa
3841aacactcact gcccagaaag cgtcggaagc tagcaaagtt cataatgaaa tcacacgaga
3901actgagcatt tccaaacact tggctcaaaa ccttattgat gatggtcctg taagaccttc
3961tgtatggaaa aagcatggtg ttgtaaatac acttagctcc tctcttcctc aaggtgatct
4021tgaaagtaat aatccttttc attgtaatat tttaatgaaa gatgacaaag atccccagtg
4081taatatattt ggtcaagact tacctgcagt accccagaga aaagaattta attttccaga
4141ggctggttcc tcttctggtg ccttattccc aagtgctgtt tcccctccag aactgcgaca
4201gagactacat ggggtagaac tcttaaaaat atttaataaa aatcaaaaat taggcagttc
4261atctactagc ataccacatc tgtcatcccc accaaccaaa ttttttgtta gtacaccatc
4321tcagccaagt tgcaaaagcc acttggaaac tggaaccaaa gatcaagaaa ctgtttgctc
4381taaagctaca gaaggagata atacagaatt tggagcattt gtaggacaca gagatagcat
4441ggatttacag aggtttaaag aaacatcaaa caagataaaa atactatcca ataacaatac
4501ttctgaaaac actttgaaac gagtgagttc tcttgctgga tttactgact gtcacagaac
4561ttccattcct gttcattcaa aacaagaaaa aatcagtaga aggccatcta ccgaagacac
4621tcatgaagta gattccaaag cagctttaat accggtttgg ttacaagata gaccatcaaa
4681cagagaaatg ccatctgaag aaggaacatt aaatggtctc acttctccat ttaagccagc
4741tatggataca aattactatt attcagctgt ggaaagaaat aacttgatga ggttatcaca
4801gagcattcca tttacacctg tgcctccaag aggggagcct gtcacagtgt atcgtttgga
4861agagagttca cccaacatac taaataacag catgtcttct tggtcacaac taggcctctg
4921tgccaaaata gagtttttaa gcaaagagga gatgggagga ggtttacgaa gagctgtcaa
4981agtacagtgt acctggtcag aacatgatat cctcaaatca gggcatcttt atattatcaa
5041atcttttctt ccagaggtgg ttaatacatg gtcaagtatt tacaaagaag atacagttct
5101gcatctctgt ctgagagaaa ttcaacaaca gagagcagca caaaagctta cgtttgcctt
5161taatcaaatg aaacccaaat ccataccata ttctccaagg ttccttgaag ttttcctgct
5221gtattgccat tcagcaggac agtggtttgc tgtggaagaa tgtatgactg gagaatttag
5281aaaatacaac aataataatg gagatgagat tattccaact aatactctgg aagagatcat
5341gctagccttt agccactgga cttacgaata tacaagaggg gagttactgg tacttgattt
5401gcaaggtgtt ggtgaaaatt tgactgaccc atctgtgata aaagcagaag aaaagagatc
5461ctgtgatatg gtttttggcc cagcaaatct aggagaagat gcaattaaaa acttcagagc
5521aaaacatcac tgtaattctt gctgtagaaa gcttaaactt ccagatctga agaggaatga
5581ttatacgcct gataaaatta tatttcctca ggatgagcct tcagatttga atcttcagcc
5641tggaaattcc accaaagaat cagaatcaac taattctgtt cgtctgatgt tattgccaac
5701tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
5761gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta

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