Construct: ORF ccsbBroad304_15096

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006181.2_s304c1, BRDN0000459711
Derived from:
ccsbBroadEn_15096
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STK32B (55351)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15096
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55351 STK32B serine/threonine kinase 32B NM_018401.3 99.9% 99.7% 592A>G
2 human 55351 STK32B serine/threonine kinase 32B NM_001345969.2 92.6% 92.5% 471_472ins90;502A>G
3 human 55351 STK32B serine/threonine kinase 32B NM_001306082.2 88.5% 88.4% 0_1ins141;451A>G
4 human 55351 STK32B serine/threonine kinase 32B XM_024454131.1 88.5% 88.4% 0_1ins141;451A>G
5 human 55351 STK32B serine/threonine kinase 32B XM_017008374.1 54.2% 51.9% (many diffs)
6 human 55351 STK32B serine/threonine kinase 32B XM_005247982.3 52.8% 52.6% 0_1ins585;7A>G
7 human 55351 STK32B serine/threonine kinase 32B XM_005247983.4 52.8% 52.6% 0_1ins585;7A>G
8 human 55351 STK32B serine/threonine kinase 32B XM_011513504.2 52.8% 52.6% 0_1ins585;7A>G
9 human 55351 STK32B serine/threonine kinase 32B XM_017008377.1 52.8% 52.6% 0_1ins585;7A>G
10 human 55351 STK32B serine/threonine kinase 32B XM_017008375.1 49.8% 42.5% (many diffs)
11 human 55351 STK32B serine/threonine kinase 32B XM_017008376.1 49.8% 42.5% (many diffs)
12 mouse 64293 Stk32b serine/threonine kinase 32B NM_022416.2 86.7% 92.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1308
ORF length:
1242
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg cgggaaccac tcccacaagc cccccgtgtt tgacgagaat gaggaagtca
121actttgacca ttttcagatt ctgcgggcca ttggtaaagg gagttttgga aaggtatgca
181tcgtgcagaa gcgagacact aagaaaatgt atgcaatgaa gtacatgaac aagcagaagt
241gcatcgagag ggatgaggtt cggaatgttt tccgggagct gcagatcatg caagggctgg
301agcacccctt cctggtcaat ctgtggtact ccttccagga tgaggaggac atgttcatgg
361tggtggacct gctcctggga ggcgacctgc gctaccatct gcagcagaat gtgcatttca
421cagaggggac tgtgaaactc tacatctgtg agctggcact ggccctggag tatcttcaga
481ggtaccacat catccacaga gacatcaagc cagacaatat cctgctggat gaacacggac
541atgttcacat tacagacttc aacatagcga cggtagtgaa aggagcagaa agggcttcct
601ccatggctgg caccaagccc tacatggctc cagaagtatt ccaggtgtac atggacggag
661gccccggata ctcgtaccct gtcgactggt ggtccctggg catcacagcc tatgagctgc
721tgcggggctg gaggccgtac gaaatccact cggtcacgcc catcgatgaa atcctcaaca
781tgttcaaggt ggagcgtgtc cactactcct ccacgtggtg caaggggatg gtggccctgc
841tgaggaagct cctgaccaag gatcctgaga gccgcgtgtc cagccttcat gacatacaga
901gcgtgcccta cttggccgac atgaactggg acgcggtgtt caagaaggca ctgatgcccg
961gctttgtgcc caataaaggg aggttgaact gcgatcccac atttgagctt gaagagatga
1021ttctagaatc caagccactt cacaaaaaga agaagcgatt ggcaaagaac agatccaggg
1081atggcacaaa ggacagctgc ccgctgaatg gacacctgca gcactgtttg gagactgtcc
1141gggaggaatt catcatattc aacagagaga agctcaggag gcagcaggga cagggcagcc
1201agctcttgga caccgacagc cgagggggag gccaggccca aagcaagctc caggacgggt
1261gcaacaacaa cctcctcacc cacacctgca cccgtggctg cagcagctgc ccaactttct
1321tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1381aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)