Construct: ORF ccsbBroad304_15209
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018944.1_s304c1, BRDN0000459428
- Derived from:
- ccsbBroadEn_15209
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- IGFN1 (91156)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15209
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91156 | IGFN1 | immunoglobulin like and fib... | NM_001367841.1 | 62.5% | 14.9% | (many diffs) |
| 2 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_017002787.1 | 22% | 5.7% | (many diffs) |
| 3 | human | 91156 | IGFN1 | immunoglobulin like and fib... | NM_001164586.2 | 21.4% | 5.6% | (many diffs) |
| 4 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_011510120.3 | 21.4% | 5.6% | (many diffs) |
| 5 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_011510121.3 | 21.4% | 5.6% | (many diffs) |
| 6 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_005245580.3 | 20.8% | 5.6% | (many diffs) |
| 7 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XR_921994.2 | 19.9% | (many diffs) | |
| 8 | human | 91156 | IGFN1 | immunoglobulin like and fib... | XM_006711618.3 | 16.5% | 6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 840
- ORF length:
- 771
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggtggcag cctatgggag agaactgggg gtgcctggag gagatgctga 121 atgaagatca gagccgggag ccccctggtc accttggtag caggagaagt ggcaaagacg 181 gcaggttgga catctatgga gagaggagag atgctacccg gagttccaca tccagataca 241 agcctggcac tggcagtttc tccaaggatg cccaaggccc catgggccac ttctcccagg 301 gcctggctga catggaagtg cagccggggg aggctgccac actctcctgt accctcacca 361 gtgacctggg acccggcacc tggtttaagg atggcgtcaa gctcaccacc caggatggag 421 tcatctttaa gcaagacggt ctcgtgcaca gcctcttcat cacgcatgtg caggggaccc 481 aagctgggag gtacaccttt gtagctggtg accagcagag cgaggccacc ctgactgtcc 541 aggattcccc taccattgct ccagatgtga cagagaaact gagagagcca ctggtggtca 601 aggctgggaa gccggtgata gtgaagatcc ccttccagag ccacctcccc attcagctgc 661 ctggaggaaa ggacggggct gagtggtggg cagcagtgac agggaggccc agtgggacct 721 gggggatggc tacacgcggc tgtgcctccc cagcgcagca ggaaggactg tggccagtac 781 agcgtgacac tgaggagtgg aggggagggc tgtgcaggcc gagctcactc tgcaagtcat 841 agacaagcct gatcccccac aaggccccat ggaggttcag gattgccata gggctggcgt 901 ctgcctccgc tggcggcccc caagggacaa tgggggccgg actgtagagt gctacgtggt 961 ggagagacgg caggctggca ggagcacttg gctgaaggtg ggcgaggccc ccgctgacag 1021 caccaccttc acggatgccc atgtggagcc aggcaggaag tataccttcc gagtgcgggc 1081 tgtgacctca gagggggctg gcgaggccct ggagtctgag gagatattgg tggctcctga 1141 ggctctcccc aaggcccctt ccgcgccagc catcctgtcg gcctccagcc agggcatcac 1201 actgacatgg acagcacctc ggggccccgg cagcgcccac atcctgggct acctgatcga 1261 gaggcgtaag aaggggagca acacctggac ggcagtgaac gaccagccgg tgcctgagag 1321 gaggtggacg gtggcggacg tgcggcaggg ctgtcagtat gagttccggg tcacagctgt 1381 ggctccctca ggtcccggag agcctggacc tccatcggat gccgtctttg ctcgggaccc 1441 catgagaccc cctgggctgg tgaggaatct ccaagtcaca gacagatcga acaccagcat 1501 cactctgagc tgggctgggc cagacaccca ggaaggggat gaagcccagg ggtatgtggt 1561 ggagctgtgc agctcagaca gtctccagtg gctcccgtgc catgtgggca ccgtgccagt 1621 caccacctac acggccaagg ggcttcggcc tggagagggc tacttcgtgc gggtgacagc 1681 agttaatgaa ggaggccaga gccagcccag tgccctggac acattagtgc aagccatgcc 1741 tgttactgtc tttcccaagt tcctcgtgga ctccagcacc aaggacttgc tgacagtcaa 1801 ggtcggggac acagttcgtg tgcccgtctc ctttgaacat gcccgcaggc cctgggccca 1861 tccactgcag gagaacgtgc gtgggacggt gacggccgag tgggaaccct ctcctgacga 1921 ggcccaggat gtcccgctgc actacgcggt gttcacacgc tcctcagcgc acggtccctg 1981 gcacgaggca gccgaccgca tctacaccaa ccgcttcacc ctcctgggca tcctccccgg 2041 ccacgaatac cacttcaggg tggtggccaa gaatgagctg ggggccagca aaccctcgga 2101 caccagccag ccctggtgca tcccccggca gcgcgacagg ttcacagtga aggctccgtg 2161 ctaccgggag cccgacctga gccagaagcc ccggttcctg gtgggcctgc ggtcccacct 2221 gctgccccag ggctgcgagt gctgcatgag ctgtgccgtg cagggctcgc cccggcccca 2281 cgtcacctgg ttcaagaatg accgcagcct ggaaggaaac cccgcggtgt acagcactga 2341 cctgctgggc gtgtgctccc tcaccatccc cagcgtatcc ccgaaggaca gcggggagta 2401 caaggctgtg gctgagaaca cgctgggcca ggcagtcagc actgccaccc tcattgtcat 2461 agaacccagc accttgccaa ctttcttgta caaagtggtt ggtaagccta tccctaaccc 2521 tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc 2581 gacaatcaac ctctggatta