Transcript: Human NM_001164586.2

Homo sapiens immunoglobulin like and fibronectin type III domain containing 1 (IGFN1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-19
Taxon:
Homo sapiens (human)
Gene:
IGFN1 (91156)
Length:
11811
CDS:
132..11258

Additional Resources:

NCBI RefSeq record:
NM_001164586.2
NBCI Gene record:
IGFN1 (91156)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147410 GTCACTCGCACCTCACAATG pXPR_003 GGG 3586 32% 12 0.3925 IGFN1 IGFN1 76823
2 BRDN0001147077 GGATGAGGCAGATTATAGGA pXPR_003 AGG 6751 61% 12 0.1642 IGFN1 IGFN1 76826
3 BRDN0001146137 GGCAGATGGTATATGCCGGG pXPR_003 GGG 2281 20% 12 -0.0095 IGFN1 IGFN1 76825
4 BRDN0001144979 ATGGAATCTAGGTACGAGGG pXPR_003 TGG 2867 26% 12 -0.0467 IGFN1 IGFN1 76824
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001164586.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000163188 GCTCCAAGCTAAGTCACTCAA pLKO.1 11463 3UTR 100% 4.950 6.930 N IGFN1 n/a
2 TRCN0000359555 AGTGGATAAGGAAGGTTATAA pLKO_005 3977 CDS 100% 15.000 10.500 N IGFN1 n/a
3 TRCN0000359626 AGTGAATAAGGCAGGTTATAG pLKO_005 4811 CDS 100% 13.200 9.240 N IGFN1 n/a
4 TRCN0000359557 AGTGAATGAAGCAGGTTATAG pLKO_005 4919 CDS 100% 13.200 9.240 N IGFN1 n/a
5 TRCN0000161407 GAGTTCCACATCCAGATACAA pLKO.1 8804 CDS 100% 5.625 3.938 N IGFN1 n/a
6 TRCN0000163405 GCCACCCTCATTGTCATAGAA pLKO.1 11226 CDS 100% 5.625 3.938 N IGFN1 n/a
7 TRCN0000162923 GAAGCCGGTGATAGTGAAGAT pLKO.1 9191 CDS 100% 4.950 3.465 N IGFN1 n/a
8 TRCN0000159798 GATGGAGTCATCTTTAAGCAA pLKO.1 8997 CDS 100% 3.000 2.100 N IGFN1 n/a
9 TRCN0000163160 GCAAGTCATAGACAAGCCTGA pLKO.1 9416 CDS 100% 2.160 1.512 N IGFN1 n/a
10 TRCN0000359627 CAGGGAGCAAGGCAGATTATA pLKO_005 4138 CDS 100% 15.000 9.000 N IGFN1 n/a
11 TRCN0000161433 GAGGAGATGCTGAATGAAGAT pLKO.1 8691 CDS 100% 4.950 2.970 N IGFN1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001164586.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12956 pDONR223 100% 23.3% 23.3% None (many diffs) n/a
2 ccsbBroad304_12956 pLX_304 0% 23.3% 23.3% V5 (many diffs) n/a
3 TRCN0000478870 TAAAAATTTCCGGTACATCCCCCA pLX_317 10.8% 23.3% 23.3% V5 (many diffs) n/a
4 ccsbBroadEn_15209 pDONR223 62.4% 21.4% 5.6% None (many diffs) n/a
5 ccsbBroad304_15209 pLX_304 0% 21.4% 5.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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