Construct: ORF ccsbBroad304_15231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018956.1_s304c1, BRDN0000459440
- Derived from:
- ccsbBroadEn_15231
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LRRK2 (120892)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15231
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | NM_198578.4 | 97.8% | 1.4% | (many diffs) |
| 2 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_005268629.4 | 97.6% | 1.5% | (many diffs) |
| 3 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537877.3 | 96.9% | 1.5% | (many diffs) |
| 4 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_024448833.1 | 83.9% | 1.4% | (many diffs) |
| 5 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XR_001748574.2 | 64.5% | (many diffs) | |
| 6 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537881.3 | 61.9% | 2.3% | (many diffs) |
| 7 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018787.1 | 59% | 1.4% | (many diffs) |
| 8 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018786.2 | 58.5% | 2.4% | (many diffs) |
| 9 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018788.2 | 50.2% | .8% | (many diffs) |
| 10 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537882.3 | 48.6% | 2.9% | (many diffs) |
| 11 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018789.2 | 48.4% | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 390
- ORF length:
- 321
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctagtggc agctgtcagg ggtgcgaaga ggacgagnaa acttctgaag 121 aagttgatag tcaggctgaa caatgtccag gaaggaaaac agatagaaac gctggtccaa 181 atcctggagg atctgctggt gttcacgtac tccgagcacg cctccaagtt atttcaaggc 241 aaaaatatcc atgtgcctct gttgatcgtc ttggactcct atatgagagt cgcgagtgtg 301 cagcaggtgg gttggtcact tctgtgcaaa ttaatagaag tctgtccagg tacaatgcaa 361 agcttaatgg gaccccagga tgttggaaat gattgggaag tccttggtgt tcaccaattg 421 attcttaaaa tgctaacagt tcataatgcc agtgtaaact tgtcagtgat tggactgaag 481 accttagatc tcctcctaac ttcaggtaaa atcaccttgc tgatattgga tgaagaaagt 541 gatattttca tgttaatttt tgatgccatg cactcatttc cagccaatga tgaagtccag 601 aaacttggat gcaaagcttt acatgtgctg tttgagagag tctcagagga gcaactgact 661 gaatttgttg agaacaaaga ttatatgata ttgttaagtg cgttaacaaa ttttaaagat 721 gaagaggaaa ttgtgcttca tgtgctgcat tgtttacatt ccctagcgat tccttagata 781 caatcttgct tgaccgtttg ggacatcaat ncttccacat gaanntgcaa aatttagaaa 841 aacacattga agtgagaaaa gaattagctg aaaaaatgag acgaacatct gttgagttgc 901 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg 961 aacaggtcac tatcagtcaa aataaaatca ttattgccat ccagctagct cagtgctcag 1021 ctgtttggcc ctcctcactg agactatttt cttaaatcaa gatttagagg aaaagaatga 1081 gaatcaagag aatgatgatg agggggaaga agataaattg ttttggctgg aagcctgtta 1141 caaagcatta acgtggcata gaaagaacaa gcacgtgcag gaggccgcat gctgggcact 1201 aaataatctc cttatgtacc aaaacagttt acatgagaag attggagatg aagatggcca 1261 tttcccagct catagggaag tgatgctctc catgctgatg cattcttcat caaaggaagt 1321 tttccaggca tctgcgaatg cattgtcaac tctcttagaa caaaatgtta atttcagaaa 1381 aatactgtta tcaaaaggaa tacacctgaa tgttttggag ttaatgcaga agcatataca 1441 ttctcctgaa gtggctgaaa gtggctgtaa aatgctaaat catctttttg aaggaagcaa 1501 cacttccctg gatataatgg cagcagtggt ccccaaaata ctaacagtta tgaaacgtca 1561 tgagacatca ttaccagtgc agctggaggc gcttcgagct attttacatt ttatagtgcc 1621 tggcatgcca gaagaatcca gggaggatac agaatttcat cataagctaa atatggttaa 1681 aaaacagtgt ttcaagaatg atattcacaa actggtccta gcagctttga acaggttcat 1741 tggaaatcct gggattcaga aatgtggatt aaaagtaatt tcttctattg tacattttcc 1801 tgatgcatta gagatgttat ccctggaagg tgctatggat tcagtgcttc acacactgca 1861 gatgtatcca gatgaccaag aaattcagtg tctgggttta agtcttatag gatacttgat 1921 tacaaagaag aatgtgttca taggaactgg acatctgctg gcaaaaattc tggtttccag 1981 cttataccga tttaaggatg ttgctgaaat acagactaaa ggatttcaga caatcttagc 2041 aatcctcaaa ttgtcagcat ctttttctaa gctgctggtg catcattcat ttgacttagt 2101 aatattccat caaatgtctt ccaatatcat ggaacaaaag gatcaacagt ttctaaacct 2161 ctgttgcaag tgttttgcaa aagtagctat ggatgattac ttaaaaaatg tgatgctaga 2221 gagagcgtgt gatcagaata acagcatcat ggttgaatgc ttgcttctat tgggagcaga 2281 tgccaatcaa gcaaaggagg gatcttcttt aatttgtcag gtatgtgaga aagagagcag 2341 tcccaaattg gtggaactct tactgaatag tggatctcgt gaacaagatg tacgaaaagc 2401 gttgacgata agcattggga aaggtgacag ccagatcatc agcttgctct taaggaggct 2461 ggccctggat gtggccaaca atagcatttg ccttggagga ttttgtatag gaaaagttga 2521 accttcttgg cttggtcctt tatttccaga taagacttct aatttaagga aacaaacaaa 2581 tatagcatct acactagcaa gaatggtgat cagatatcag atgaaaagtg ctgtggaaga 2641 aggaacagcc tcaggcagcg atggaaattt ttctgaagat gtgctgtcta aatttgatga 2701 atggaccttt attcctgact cttctatgga cagtgtgttt gctcaaagtg atgacctgga 2761 tagtgaagga agtgaaggct catttcttgt gaaaaagaaa tctaattcaa ttagtgtagg 2821 agaattttac cgagatgccg tattacagcg ttgctcacca aatttgcaaa gacattccaa 2881 ttccttgggg cccatttttg atcatgaaga tttactgaag cgaaaaagaa aaatattatc 2941 ttcagatgat tcactcaggt catcaaaact tcaatcccat atgaggcatt cagacagcat 3001 ttcttctctg gcttctgaga gagaatatat tacatcacta gacctttcag caaatgaact 3061 aagagatatt gatgccctaa gccagaaatg ctgtataagt gttcatttgg agcatcttga 3121 aaagctggag cttcaccaga atgcactcac gagctttcca caacagctat gtgaaactct 3181 gaagagtttg acacatttgg acttgcacag taataaattt acatcatttc cttcttattt 3241 gttgaaaatg agttgtattg ctaatcttga tgtctctcga aatgacattg gaccctcagt 3301 ggttttagat cctacagtga aatgtccaac tctgaaacag tttaacctgt catataacca 3361 gctgtctttt gtacctgaga acctcactga tgtggtagag aaactggagc agctcatttt 3421 agaaggaaat aaaatatcag ggatatgctc ccccttgaga ctgaaggaac tgaagatttt 3481 aaaccttagt aagaaccaca tttcatccct atcagagaac tttcttgagg cttgtcctaa 3541 agtggagagt ttcagtgcca gaatgaattt tcttgctgct atgcctttct tgcctccttc 3601 tatgacaatc ctaaaattat ctcagaacaa attttcctgt attccagaag caattttaaa 3661 tcttccacac ttgcggtctt tagatatgag cagcaatgat attcagtacc taccaggtcc 3721 cgcacactgg aaatctttga acttaaggga actcttattt agccataatc agatcagcat 3781 cttggacttg agtgaaaaag catatttatg gtctagagta gagaaactgc atctttctca 3841 caataaactg aaagagattc ctcctgagat tggctgtctt gaaaatctga catctctgga 3901 tgtcagttac aacttggaac taagatcctt tcccaatgaa atggggaaat taagcaaaat 3961 atgggatctt cctttggatg aactgcatct taactttgat tttaaacata taggatgtaa 4021 agccaaagac atcataaggt ttcttcaaca gcgattaaaa aaggctgtgc cttataaccg 4081 aatgaaactt atgattgtgg gaaatactgg gagtggtaaa accaccttat tgcagcaatt 4141 aatgaaaacc aagaaatcag atcttggaat gcaaagtgcc acagttggca tagatgtgaa 4201 agactggcct atccaaataa gagacaaaag aaagagagat ctcgtcctaa atgtgtggga 4261 ttttgcaggt cgtgaggaat tctatagtac tcatccccat tttatgacgc agcgagcatt 4321 gtaccttgct gtctatgacc tcagcaaggg acaggctgaa gttgatgcca tgaagccttg 4381 gctcttcaat ataaaggctc gcgcttcttc ttcccctgtg attctcgttg gcacacattt 4441 ggatgtttct gatgagaagc aacgcaaagc ctgcatgagt aaaatcacca aggaactcct 4501 gaataagcga gggttccctg ccatacgaga ttaccacttt gtgaatgcca ccgaggaatc 4561 tgatgctttg gcaaaacttc ggaaaaccat cataaacgag agccttaatt tcaagatccg 4621 agatcagctt gttgttggac agctgattcc agactgctat gtagaacttg aaaaaatcat 4681 tttatcggag cgtaaaaatg tgccaattga atttcccgta attgaccgga aacgattatt 4741 acaactagtg agagaaaatc agctgcagtt agatgaaaat gagcttcctc acgcagttca 4801 ctttctaaat gaatcaggag tccttcttca ttttcaagac ccagcactgc agttaagtga 4861 cttgtacttt gtggaaccca agtggctttg taaaatcatg gcacagattt tgacagtgaa 4921 agtggaaggt tgtccaaaac accctaaggg aattatttcg cgtagagatg tggaaaaatt 4981 tctttcaaag aaaaggaaat ttccaaagaa ctacatgaca cagtatttta agctcctaga 5041 aaaattccag attgctttgc caataggaga agaatatttg ctggttccaa gcagtttgtc 5101 tgaccacagg cctgtgatag agcttcccca ttgtgagaac tctgaaatta tcatccgact 5161 atatgaaatg ccttattttc caatgggatt ttggtcaaga ttaatcaatc gattacttga 5221 gatttcacct tacatgcttt cagggagaga acgagcactt cgcccaaaca gaatgtattg 5281 gcgacaaggc atttacttaa attggtctcc tgaagcttat tgtctggtag gatctgaagt 5341 cttagacaat catccagaga gtttcttaaa aattacagtt ccttcttgta gaaaaggctg 5401 tattcttttg ggccaagttg tggaccacat tgattctctc atggaagaat ggtttcctgg 5461 gttgctggag attgatattt gtggtgaagg agaaactctg ttgaagaaat gggcattata 5521 tagttttaat gatggcgaag aacatcaaaa aatcttactt gatgacttga tgaagaaagc 5581 agaggaagga gatctcttag taaatccaga tcaaccaagg ctcaccattc caatatctca 5641 gattgcccct gacttgattt tggctgacct gcctagaaat attatgttga ataatgatga 5701 gttggaattt gaacaagctc cagagtttct cctaggtgat ggcagttttg gatcagttta 5761 ccgagcagcc tatgaaggag aagaagtggc tgtgaagatt tttaataaac atacatcact 5821 caggctgtta agacaagagc ttgtggtgct ttgccacctc caccacccca gtttgatatc 5881 tttgctggca gctgggattc gtccccggat gttggtgatg gagttagcct ccaagggttc 5941 cttggatcgc ctgcttcagc aggacaaagc cagcctcact agaaccctac agcacaggat 6001 tgcactccac gtagctgatg gtttgagata cctccactca gccatgatta tataccgaga 6061 cctgaaaccc cacaatgtgc tgcttttcac actgtatccc aatgctgcca tcattgcaaa 6121 gattgctgac tacggcattg ctcagtactg ctgtagaatg gggataaaaa catcagaggg 6181 cacaccaggg tttcgtgcac ctgaagttgc cagaggaaat gtcatttata accaacaggc 6241 tgatgtttat tcatttggtt tactactcta tgacattttg acaactggag gtagaatagt 6301 agagggtttg aagtttccaa atgagtttga tgaattagaa atacaaggaa aattacctga 6361 tccagttaaa gaatatggtt gtgccccatg gcctatggtt gaaaaattaa ttaaacagtg 6421 tttgaaagaa aatcctcaag aaaggcctac ttctgcccag gtctttgaca ttttgaattc 6481 agctgaatta gtctgtctga cgagacgcat tttattacct aaaaacgtaa ttgttgaatg 6541 catggttgct acacatcaca acagcaggaa tgcaagcatt tggctgggct gtgggcacac 6601 cgacagagga cagctctcat ttcttgactt aaatactgaa ggatacactt ctgaggaagt 6661 tgctgatagt agaatattgt gcttagcctt ggtgcatctt cctgttgaaa aggaaagctg 6721 gattgtgtct gggacacagt ctggtactct cctggtcatc aataccgaag atgggaaaaa 6781 gagacatacc ctagaaaaga tgactgattc tgtcacttgt ttgtattgca attccttttc 6841 caagcaaagc aaacaaaaaa attttctttt ggttggaacc gctgatggca agttagcaat 6901 ttttgaagat aagactgtta agcttaaagg agctgctcct ttgaagatac taaatatagg 6961 aaatgtcagt actccattga tgtgtttgag tgaatccaca aattcaacgg aaagaaatgt 7021 aatgtgggga ggatgtggca caaagatttt ctccttttct aatgatttca ccattcagaa 7081 actcattgag acaagaacaa gccaactgtt ttcttatgca gctttcagtg attccaacat 7141 cataacagtg gtggtagaca ctgctctcta tattgctaag caaaatagcc ctgttgtgga 7201 agtgtgggat aagaaaactg aaaaactctg tggactaata gactgcgtgc actttttaag 7261 ggaggtaacg gtaaaagaaa acaaggaatc aaaacacaaa atgtcttatt ctgggagagt 7321 gaaaaccctc tgccttcaga agaacactgc tctttggata ggaactggag gaggccatat 7381 tttactcctg gatctttcaa ctcgtcgact tatacgtgta atttacaact tttgtaattc 7441 ggtcagagtc atgatgacag cacagctagg aagccttaaa aatgtcatgc tggtattggg 7501 ctacaaccgg aaaaatactg aaggtacaca aaagcagaaa gagatacaat cttgcttgac 7561 cgtttgggac atcaatcttc cacatgaagt gcaaaattta gaaaaacaca ttgaagtgag 7621 aaaagaatta gctgaaaaaa tgagacgaac atctgttgag ttgccaactt tcttgtacaa 7681 agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc 7741 tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta