Construct: ORF ccsbBroad304_15251

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018923.1_s304c1, BRDN0000459407
Derived from:
ccsbBroadEn_15251
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DGKK (139189)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_15251
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 139189 DGKK diacylglycerol kinase kappa NM_001013742.4 99.5% 18.5% (many diffs)
2 human 139189 DGKK diacylglycerol kinase kappa XM_017029268.2 97.2% 19% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
882
ORF length:
813
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaccgcgga gctgccgcag cccagggcac tgccccgcct caggatggag
121agcagcccgc tgagtctcca gagcctccgc cgccttggcc gccgccgcca ccaccaccgg
181ctccgccgcc ggctccgccg ctgctctccg aggcttcgcc agaacccata ccagagccct
241gtccagagct tgctccaggt ccctgtccag aggcgacctc agaatcagcc acagaactgt
301acacagaacc gaccccagaa ccagccacag agccggcctc agaaccggcc ccagaacctg
361ccacagagcc ggccccagaa cctgccacag agccggcccc agaaccggcc ccagaacctg
421ccacagagtc tgccccagag ccgactccag aacctgccct agagtcggtc ccagagccgg
481ccccagagct gactccagaa gttgccccag agctggcccc agagccgacc ccagaacctg
541tgacagagct ggccccagag ttctgccctg aggcggctcc agagttccgt ccaagtccag
601caccatgtct attgcaatgt ccggtggaca ctcgagagag aggtctaaag acctcgccat
661cgccatcgcc atcgccatcg cccagaacgc caatgtcgtg gtccagaata aagaaaatat
721tgaaggaagg acctatgctg aagaactgta actctttcag agatggaagc ttagatattt
781tctggttcaa ggacagaagc tctactttgc acaccatccc gcgtttgcac actttgaaac
841gattgatctg tctcaagcca ctggtggcag aaaggcagct gtagaaaccc tttggccaac
901aggattgttg ttattacacc acaacgaaaa atcactctgg ctgcacccaa ccggaaagac
961atggaagaat ggattaacat cataaaaacc atccaacagg gagaaattta taagatacct
1021gcagcagaaa acaacccttt tcttgttgga atgcattgtt ggtactccag ttacagccac
1081cggacccagc actgcaatgt ttgtcgagag agcattcctg ccttatctag agatgccatc
1141atctgtgaag tgtgcaaagt gaaatctcac agattgtgtg ctttgagagc aagcaaagac
1201tgcaagtgga atacattgtc tatcactgat gacctccttc tgcctgcaga tgaagtaaac
1261atgccccatc aatgggtaga aggaaacatg cctgtcagct ctcagtgtgc agtgtgtcat
1321gagagctgtg gcagttatca aagacttcaa gacttccgct gcctgtggtg taattctacg
1381gtgcatgatg actgtaggag acggttttcc aaggaatgtt gcttcagaag ccatcgctca
1441tcagtcattc ctcccactgc tctaagcgac cccaaaggcg atggccaatt agtagtatct
1501tcagacttct ggaatcttga ttggtcatca gcctgttcat gtcccttgct catcttcatc
1561aactccaaaa gtggcgatca tcaggggatc gtcttcctcc gaaaattcaa gcaatacctt
1621aacccatctc aagtgttcga cttattgaag ggtggacctg aagcagggct gtctatgttc
1681aagaactttg ctcgctttcg cattctggtt tgtggtggag atggcagcgt gagctgggtc
1741ttatctctga ttgatgcctt tggattacat gaaaagtgtc agttggcagt catcccactt
1801ggaaccggca atgatctggc tcgtgttctg ggctggggtg cattctggaa caaaagcaag
1861tcacctctgg acatcctcaa cagagtggag caggctagtg tgaggatcct agacagatgg
1921agtgtgatga ttcgtgagac tcccagacaa accccgctgc taaaaggaca ggttgaaatg
1981gatgtaccac gatttgaggc tgctgccatc caacacttag aatctgcagc caccgagttg
2041aacaaaatcc tgaaggccaa gtaccccaca gagatgatca tcgcaaccag attcttgtgt
2101tcagctgtgg aagattttgt ggttgatatt gtaaaggcct ggggtcagat aaaacagaac
2161aacactgcaa tagtgtctgt gattttgaaa agtgacttaa tgtatgatag gctcagtgtc
2221ctgatcgatg tcctggctga ggaggcagca gctacttctg ctgaaaaaag tgctacagaa
2281tatgcagaca gcagcaaggc agataggaag cccttcattc ctcaaataga ccacatagcc
2341aagtgcaagt tggagctggc tacaaaggcc cagagtctcc agaaatcctt gaaactcatc
2401atatttcaag ttgaacaagc tctggatgag gaaagcagac agacaatatc tgttaagaac
2461tttagttcaa ctttcttcct ggaagatgac ccagaagata ttaaccagac aagcccacga
2521cgccgttctc gtcgtggcac tttgtcttct atatcttctc tcaaaagtga ggacctggac
2581aaccttaact tggatcactt acattttaca cctgaatcta tacgcttcaa agaaaaatgt
2641gtcatgaaca actacttcgg aattggactg gatgctaaaa tttctctgga cttcaacacc
2701agaagagatg aacacccagg gcaatacaat agccgcctta agaacaagat gtggtatggc
2761cttctgggaa ccaaagaact tttgcagcgc tcttacagga aactggaaga acgagtgcat
2821ttggagtgtg atggagaaac catctccttg ccaaacctgc aaggcattgt agtgctcaac
2881attaccagct atgctggagg tatcaacttc tggggaagca acacagcaac cacggaatat
2941gaggctcctg caatcgatga tgggaaactg gaggtggtgg caatctttgg ttctgtgcag
3001atggcaatgt cccgtatcat caacctgcat catcatcgca ttgcccagtg ccatgaggtg
3061atgataacca ttgattgtga agaaggtatc ccagggcagg tgatggggga ggcttggatt
3121cagagaccag gctttatcaa aaatagatac aagaacgctg cccagatgct gacaagagat
3181cgggactttg agaactcaat gaaaatgtgg gaatacaagc atactgaaat tcaagctgcc
3241cctcaacccc agctggactt ccaggactct caagagagcc tctctgacga ggagtatgcc
3301cagatgcagc acttagctcg gcttgcagaa aacctcatca gcaaacttaa tgacctgagc
3361aagatccacc agcatgtgtc tgtcctcatg ggttctgtga atgccagcgc taacatcctg
3421aataatatat tttacggcca agacagtggc aatgagatgg gtgcagcttc ctgtattccc
3481attgaaactc taagcagaaa tgatgccgta gatgttacat ttagtcttaa aggtctctac
3541gatgacacca cagctttcct ggatgaaaag ctgctgagaa gtgctgagga tgagactgca
3601ctacaaagcg ccctggatgc catgaataag gagttcaaaa agctatctga gattgactgg
3661atgaatccaa tctttgttcc agaggaaaaa tcttcggaca ctgacagtag aagcctcagg
3721ctgaaaatta agttccccaa attgggaaag aaaaaggtag aagaggaacg caagcctaaa
3781tcaggccaga gtgtccagag ttttattggc aatttatggc accgcagaca tcgtgaagat
3841gaagcagagg gtgatgatcc tctaacacca tcgagatctc aactgttgcc aactttcttg
3901tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
3961tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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