Construct: ORF ccsbBroad304_15266
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008921.1_s304c1, BRDN0000408745
- Derived from:
- ccsbBroadEn_15266
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PROM2 (150696)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15266
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150696 | PROM2 | prominin 2 | NM_001165977.3 | 99.4% | 37.8% | (many diffs) |
| 2 | human | 150696 | PROM2 | prominin 2 | NM_001165978.3 | 99.4% | 37.8% | (many diffs) |
| 3 | human | 150696 | PROM2 | prominin 2 | NM_144707.4 | 99.4% | 37.8% | (many diffs) |
| 4 | human | 150696 | PROM2 | prominin 2 | NM_001321070.2 | 58.3% | 16.2% | (many diffs) |
| 5 | human | 150696 | PROM2 | prominin 2 | XM_006712301.2 | 58.3% | 16.2% | (many diffs) |
| 6 | human | 150696 | PROM2 | prominin 2 | XM_011510672.2 | 57.7% | 64.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1035
- ORF length:
- 966
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagcacaca ctggctctgc tggctcccct gctgggcctg ggcctggggc 121 tggccctgag tcagctggct gcaggggcca cagactgcaa gttccttggc ccggcagagc 181 acctgacatt caccccagca gccagggccc ggtggctggc ccctcgagtt cgtgcgccag 241 gactcctgga ctccctctat ggcaccgtgc gccgcttcct ctcggtggtg cagctcaatc 301 ctttcccttc agagttggta aaggccctac tgaatgagct ggcctccgtg aaggtgaatg 361 aggtggtgcg gtacgaggcg ggctacgtgg tatgcgctgt gatcgcgggc ctctacctgc 421 tgctggtgcc cactgccggg ctttgcttct gctgctgccg ctgccaccgg cgctgcgggg 481 gacgagtgaa gacagagcac aaggcgctgg cctgtgagcg cgcggccctc atggtcttcc 541 tgctgctgac caccctcttg ctgctgattg gtgtggtctg tgcctttgtc accaaccagc 601 gcacgcatga acagatgggc cccagcatcg aggccatgcc tgagaccctg ctcagcctct 661 ggggcctggt ctctgatgtc ccccaagtgt tggaggccgt ggcacagcaa ttctccctgc 721 cccaggagca agtctcagag gagctggaaa gtgttggtgt gagcattggg agcgcgatcc 781 acactcagct caggagctcc gtgtacccct tgctggcggc cgtgggcagt ttgggccagg 841 tcctgcaggt ctccgtgcac cacctgcaaa ccttgaatgc tacagtggta gagctgcagg 901 ccgggcagca ggacctggag ccagccatcc gggaacaccg ggaccgcctc cttgagctgc 961 tgcaggaggc caggtgccct cgagattgtg caggggccct gagctgggcc cgcaccctgg 1021 agctgggtgc tgactgaggc caggtgccct ctgtggacca tgtcctgcac cagctaaaag 1081 gtgtccccga ggccaacttc tccagcatgg tccaggagga gaacagcacc ttcaacgccc 1141 ttccagccct ggctgccatg cagacatcca gcgtggtgca agagctgaag aaggcagtgg 1201 cccagcagcc ggaaggggtg aggacactgg ctgaagggtt cccgggcttg gaggcagctt 1261 cccgctgggc ccaggcactg caggaggtgg aggagagcag ccgcccctac ctgcaggagg 1321 tgcagagata cgagacctac aggtggatcg tgggctgcgt gctgtgctcc gtggtcctat 1381 tcgtggtgct ctgcaacctg ctgggcctca atctgggcat ctggggcctg tctgccaggg 1441 acgaccccag ccacccagaa gccaagggcg aggctggagc ccgcttcctc atggcaggtg 1501 tgggcctcag cttcctcttt gctgcacccc tcatcctcct ggtgttcgcc accttcctgg 1561 tgggtggcaa cgtgcagacg ctggtgtgcc ggagctggga gaacggcgag ctctttgagt 1621 ttgcagacac cccagggaac ctgcccccgt ccatgaacct gtcgcaactt cttggcctga 1681 ggaagaacat cagcatccac caagcctatc agcagtgcaa ggaaggggca gcgctctgga 1741 cagtcctgca gctcaacgac tcctacgacc tggaggagca cctggatatc aaccagaata 1801 ccaacaagct acggcaggag ttgcagagcc tgaaagtaga cacacagagc ctggacctgc 1861 tgagctcagc cgcccgccgg gacctggagg ccctgcagag cagtgggctt cagcgcatcc 1921 actaccccga cttcctcgac cagatccaga ggcccgtggt gaagaccagc atggagcagc 1981 tggcccagga gctgcaagga ctggcccagg cccaagacaa ttctgtgctg gggcagcggc 2041 tgcaggagga ggcccaagga ctcagaaacc ttcaccagga gaaggtcgtc ccccagcaga 2101 gccttgtggc aaagctcaac ctcagcgtca gggccctgga gtcctctgcc ccgaatctcc 2161 agctggagac ctcagatgtc ctagccaatg tcacctacct gaaaggagag ctgcctgcct 2221 gggcagccag gatcctgagg aatgtgagtg agtgtttcct ggcccgggag atgggctact 2281 tctcccagta cgtggcctgg gtgagagagg aggtgactca gcgcattgcc acctgccagc 2341 ccctctccgg agccctggac aacagccgtg tgatcctgtg tgacatgatg gctgacccct 2401 ggaatgcctt ctggttctgc ctggcatggt gcaccttctt cctgatcccc agcatcatct 2461 ttgccgtcaa gacctccaaa tacttccgtc ctatccggaa acgcctcagc tccaccagct 2521 ctgaggagac tcagctcttc cacatccccc gggttacctc cctgaagctg ttgccaactt 2581 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 2641 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta