Transcript: Human NM_001165978.3

Homo sapiens prominin 2 (PROM2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
PROM2 (150696)
Length:
4731
CDS:
138..2642

Additional Resources:

NCBI RefSeq record:
NM_001165978.3
NBCI Gene record:
PROM2 (150696)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001165978.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000420256 ACAGACCTCAGATCCATTAAA pLKO_005 3709 3UTR 100% 15.000 10.500 N PROM2 n/a
2 TRCN0000164968 GATGGTGTTGGTGTGAGCATT pLKO.1 816 CDS 100% 4.950 3.465 N PROM2 n/a
3 TRCN0000165855 GCACCTGGATATCAACCAGTA pLKO.1 1847 CDS 100% 4.050 2.835 N PROM2 n/a
4 TRCN0000165765 CCTTTCCCTTCAGAGTTGGTA pLKO.1 369 CDS 100% 3.000 2.100 N PROM2 n/a
5 TRCN0000166447 CCTCCAAATACTTCCGTCCTA pLKO.1 2542 CDS 100% 2.640 1.848 N PROM2 n/a
6 TRCN0000165090 GCCTGAAAGTAGACACACAGA pLKO.1 1897 CDS 100% 2.640 1.848 N PROM2 n/a
7 TRCN0000160170 CCTCTATTCAAAGGCAGAAAT pLKO.1 4202 3UTR 100% 13.200 7.920 N PROM2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001165978.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15266 pDONR223 90% 99.4% 37.8% None (many diffs) n/a
2 ccsbBroad304_15266 pLX_304 0% 99.4% 37.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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