Construct: ORF ccsbBroad304_15267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016904.1_s304c1, BRDN0000460226
- Derived from:
- ccsbBroadEn_15267
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPP1R1C (151242)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15267
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NM_001080545.2 | 99.3% | 54% | 166_167insG;181_182insG |
| 2 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NM_001261425.2 | 99.3% | 54% | 166_167insG;181_182insG |
| 3 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NM_001261424.2 | 93.4% | 50.8% | 79_99del;187_188insG;202_203insG |
| 4 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | XM_017003451.1 | 70.1% | 17.3% | (many diffs) |
| 5 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NR_048567.1 | 24.1% | (many diffs) | |
| 6 | human | 151242 | PPP1R1C | protein phosphatase 1 regul... | NR_048566.2 | 10.2% | (many diffs) | |
| 7 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NM_172420.4 | 86.9% | 50.4% | (many diffs) |
| 8 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NM_001290743.1 | 74% | 59.5% | (many diffs) |
| 9 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NM_001290744.1 | 61.3% | 48.9% | (many diffs) |
| 10 | mouse | 75276 | Ppp1r1c | protein phosphatase 1, regu... | NR_110980.1 | 6.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 306
- ORF length:
- 237
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagcccaac agtcccaaaa agatacagtt tgccgtgcct gtattccaga 121 gtcagattgc acctgaagca gcagagcaga tcaggaaaag aagacctaca ccagcatcac 181 ttgtgattct caatgagcat aaccccccag aaatagatga caagaggggg cccagacaca 241 caaggggaat gtacagaatg catcccctaa gcaaaggaag cagagtgtgt acacaccacc 301 caccataaaa ggggttaagc atctgaaagg ccagaatgaa tcagcattcc ctgaagaaga 361 agaaggcacc aatgaaagag aggagcagcg ggaccatttg ccaactttct tgtacaaagt 421 ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag 481 cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta